biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 36:351, 1994 | DOI: 10.1007/BF02920930

Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata andM. balbisiana)

J. Doležel1, M. Doleželová1, F. J. Novák2
1 Department of Plant Biotechnology, Institute of Experimental Botany, Olomouc, Czech Republic
2 Plant Breeding Unit, Joint FAO/IAEA Programme, IAEA Laboratories, Seibersdorf, Austria

Cell nuclei were isolated from leaf tissues of wild banana (Musa balbisiana, M. acuminata ssp.banksii andM. acuminata ssp.errans) and of the two vegetative clones of diploid cultivar "Pisang Mas". Relative fluorescence intensity was measured on propidium iodide-stained nuclei by flow cytometry. Nuclei isolated fromGlycine max with known nuclear genome size were used as internal standard to determine nuclear DNA content ofMusa in absolute units. The results of the study showed that the size of nuclear genome ofMusa is smaller than previously estimated. In general, it is smaller in comparison with many other angiosperms. Furthermore, it was found that nuclear DNA content ofM. balbisiana (genome BB) is significantly lower than that ofM. acuminata subspecies and cultivars (genome AA). This finding should permit estimation of genome composition in triploidMusa clones with expected hybrid composition. Flow cytometry is proposed as a useful technique with potential applications in taxonomy, breeding and biotechnology ofMusa.

Received: November 25, 1993; Accepted: January 12, 1994; Published: September 1, 1994  Show citation

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Doležel, J., Doleželová, M., & Novák, F.J. (1994). Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata andM. balbisiana). Biologia plantarum, 36(3), 351. doi: 10.1007/BF02920930
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