biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:541-550, 2005 | DOI: 10.1007/s10535-005-0048-z

Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes

S. Agarwal1, R. K. Sairam1,*, G. C. Srivastava1, R. C. Meena1
1 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India

Abscisic acid (ABA) and salicylic acid (SA) were sprayed on leaves of wheat genotypes C 306 and Hira at 25 and 40 d after sowing under moderate water stress (-0.8 MPa) imposed by adding PEG-6000 in nutrient solution. ABA and SA increased the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in comparison to unsprayed control plants. Both ABA and SA treatments decreased the contents of hydrogen peroxide and thiobarbituric acid reactive substances, a measure of lipid peroxidation, compared to unsprayed plants. The beneficial effect of increase in antioxidant enzymes activity and decrease in oxidative stress was reflected in increase in chlorophyll and carotenoid contents, relative water content, membrane stability index, leaf area and total biomass over control plants. The lower concentrations of ABA (0.5 mM) and SA (1.0 mM) were generally more effective than higher concentrations.

Keywords: ascorbate peroxidase; catalase; glutathione reductase; hydrogen peroxide; lipid peroxidation; membrane stability index; superoxide dismutase; Triticum aestivum
Subjects: abscisic acid (ABA); antioxidants, enzymes; antioxidants, salicylic acid; ascorbate peroxidase; carotenoids, antioxidant enzymes, salt stress; carotenoids, salt stress; catalase; cultivar and genotype differences, antioxidants; glutathione reductase; hydrogen peroxide; lipid peroxidation; membrane stability index (MSI); oxidative stress, antioxidants; reactive oxygen species (ROS); relative water content; salicylic acid; salt stress, antioxidant enzymes; superoxide dismutase; Triticum aestivum; wheat, antioxidants

Received: June 11, 2004; Accepted: March 2, 2005; Published: December 1, 2005  Show citation

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Agarwal, S., Sairam, R.K., Srivastava, G.C., & Meena, R.C. (2005). Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biologia plantarum, 49(4), 541-550. doi: 10.1007/s10535-005-0048-z
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