biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:180-186, 2006 | DOI: 10.1007/s10535-006-0004-6

Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision

G. Q. Zhang1, D. Q. Zhang2, G. X. Tang1, Y. He1, W. J. Zhou1,*
1 Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
2 Institute of Crop Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

The present study evaluated the effects of chilling, partial desiccation, cotyledon excision and successive subculture of microspore-derived embryos on plant development in oilseed rape (Brassica napus L.). The results showed that out of the five media, all the genotypes showed the best response when the embryos were cultured on the half-strength Murashige and Skoog medium with 2.0 mg dm-3 benzylaminopurine. A cold treatment for 3 or 5 d further increased frequencies of embryo germination (90.0 %) and plantlet development (58.46 %). Desiccation for one day also increased the embryo germination and plantlet development in all genotypes tested. Cutting the cotyledons of the embryos at late cotyledonary stage significantly increased the frequency of plantlet development. The highest rate of plantlet development was obtained from cultures of embryos sampled with size of less than 4.0 mm. The successive subculture further improved the germination and development of plantlets from embryos. In the genotype ZJU452, the rate of plantlet development reached 99.78 % after the second subculture of embryos.

Keywords: auxin; Brassica napus L.; cold treatment; cytokinin; embryogenesis; gibberellin; successive subculture
Subjects: auxins; Brassica napus; cold pretreatment, treatment; cotyledon; cultivar and genotype differences; cytokinins; desiccation; germination; gibberellic acid, gibberellins; growth analysis, plant development, biomass and yield enhancement; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Murashige and Skoog; oilseed rape; temperature, low, stress

Received: July 13, 2004; Accepted: June 4, 2005; Published: June 1, 2006  Show citation

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Zhang, G.Q., Zhang, D.Q., Tang, G.X., He, Y., & Zhou, W.J. (2006). Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision. Biologia plantarum, 50(2), 180-186. doi: 10.1007/s10535-006-0004-6
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    References

    1. Albercht, S.C., Mollers, C., Robbelen, G.: Selection in vitro for erucic-acid content in segregating populations of microspore-derived embryoids of Brassica napus.-Plant Breed. 114: 210-214, 1995. Go to original source...
    2. Baillie, A.M.K., Epp, D.J., Hutheson, D., Keller, W.A.: In vitro culture of isolated microspores and regeneration of plants in Brassica campestris.-Plant Cell Rep. 11: 234-237, 1992. Go to original source...
    3. Burnett, L., Yarrow, S., Huang, B.: Embryogenesis and plant regeneration from isolated microspore of Brassica rapa L. ssp. oleifera.-Plant Cell Rep. 11: 215-218, 1992. Go to original source...
    4. Calic, D., Zdravkovic-Korac, S., Jevremovic, S., Guc-Scekic, M., Radojevic, Lj.: Efficient haploid induction in microspore suspension culture of Aesculus hippocastanum and karyotype analysis.-Biol. Plant. 47: 289-292, 2003/4. Go to original source...
    5. Chuong, P.V., Beversdorf, W.D.: High frequency embryogenesis through isolated microspore culture in Brassica napus L. and B. carinata Braun.-Plant Sci. 39: 219-226, 1985. Go to original source...
    6. Dirk, B.H., Roger, M.M., Richard, P.P.: Hormones in zygotic and microspore embryos of Brassica napus.-Plant Growth Regul. 35: 47-58, 2001.
    7. Ferrie, A.M.R., Epp, D.J., Keller, W.A.: Evaluation of Brassica rapa L. genotypes for microspore culture and identification of highly embryogenic line.-Plant Cell Rep. 14: 580-584, 1995. Go to original source...
    8. Ferrie, A.M.R., Keller, W.A.: Development of methodology and applications of doubled haploids in Brassica rapa.-Proceedings of the 9th International Rapeseed Congress. Pp. 805-809. GCIRC, Cambridge 1995.
    9. Gamborg, O.L., Miller, R.A., Ojima, K: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968. Go to original source...
    10. Gland-Zwerger, A.: Culture conditions affecting induction and regeneration in isolated microspore cultures of different Brassica species.-Proceedings of the 9th International Rapeseed Congress. Pp. 799-801. GCIRC, Cambridge 1995.
    11. Gland-Zwerger, A., Lichter, R., Schweiger, H.G.: Genetic and exogenous factors affecting embryogenesis in isolated microspore culture of Brassica napus L.-J. Plant Physiol. 132: 613-617, 1988. Go to original source...
    12. Gu, H.H., Hagberg, P., Zhou, W.J.: Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.).-Plant Growth Regul. 42: 137-143, 2004a. Go to original source...
    13. Gu, H.H., Tang, G.X., Zhang, G.Q., Zhou, W.J.: Embryogenesis and plant regeneration from isolated microspores of winter cauliflower (Brassica oleracea var. botrytis).-J. Zhejiang Univ. (Agr. Life Sci.) 30: 34-38, 2004b.
    14. Gu, H.H., Zhang, D.Q., Zhou W.J.: Effects of medium renovation and colchicine treatment on embryogenesis of isolated microspores in Brassica rapa ssp. chinensis.-Acta agron. sin. 30: 78-81, 2004c.
    15. Gu, H.H., Zhou, W.J., Hagberg, P.: High frequency spontaneous production of doubled haploid plants from microspore culture in Brassica rapa ssp. chinensis.-Euphytica 134: 239-245, 2003a. Go to original source...
    16. Gu, H.H., Zhu, J.Q., Zhang, G.Q., Zhou, W.J.: Microspore culture and ploidy identification of regenerated plants in Chinese flowering cabbage (Brassica rapa ssp. parachinensis).-J. agr. Biotechnol. 11: 681-685, 2003b.
    17. Hansen, M.: ABA treatment and desiccation of microspore-derived embryos of cabbage (Brassica oleracea ssp. capitata L.) improves plant development.-J. Plant Physiol. 156: 164-167, 2000. Go to original source...
    18. Hansen, M., Svinnset, K.: Microspore culture of swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned media.-Plant Cell Rep. 12: 496-500, 1993. Go to original source...
    19. Kott, L.S., Beversdorf, W.D.: Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection.-Plant Cell Tissue Organ Cult. 23: 187-192, 1990. Go to original source...
    20. Li, H.Z., Zhou, W.J., Zhang, Z.J., Gu, H.H., Takeuchi, Y., Yoneyama, K.: Effect of γ-radiation on development, yield and quality of microtubers in vitro in Solanum tuberosum L.-Biol. Plant. 49: 625-628, 2005. Go to original source...
    21. Lichter, R.: Induction of haploid plants from isolated pollen of Brassica napus.-Z. Pflanzenphysiol. 105: 427-434, 1982. Go to original source...
    22. Lichter, R.: Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species.-Plant Breed. 103: 119-123, 1989. Go to original source...
    23. Mathias, R.: An improved in vitro culture procedure for embryoids derived from isolated microspore of rape (Brassica napus L.).-Plant Breed. 100: 320-332, 1998. Go to original source...
    24. Momoh, E.J.J., Zhou, W.J., Kristiansson, B.: Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress.-Weed Res. 42: 446-455, 2002. Go to original source...
    25. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    26. Niu, Y.Z., Liu, Y.Z., Wang L.Z., Yuan, Y.X., Li, S.C., Fang, Q.J.: A preliminary study on isolated microspore culture and plant regeneration of resynthesized Brassica napus.-J. Sichuan agr. Univ. 17: 167-171, 1999.
    27. Polsoni, L., Kott, L.S., Beversdorf, W.D.: Large-scale microspore culture technique for mutation-selection studies in Brassica napus.-Can. J. Bot. 66: 1681-1685, 1988. Go to original source...
    28. Rajcan, I., Kasha, K.J., Kott, L.S., Beversdorf, W.D.: Evaluation of cytoplasmic effects on agronomic and seed quality traits in two doubled haploid populations of Brassica napus L.-Euphytica 123: 401-409, 2002. Go to original source...
    29. Sato, S., Katoh, N., Iwai, S., Hagimori, M.: Effect of low temperature pretreatment of buds or inflorescence on isolated microspore culture in Brassica rapa (syn. B. campestris).-Breed. Sci. 52: 23-26, 2002. Go to original source...
    30. SPSS: SPSS for Windows, version 10.0. SPSS Inc, Chicago, Chicago 1999.
    31. Swanson, E.B., Coumans, M.P., Wu, S.C., Barshy, T.L., Beversdorf, W.D.: Efficient isolation of microspore and production of microspore-derived embryos from Brassica napus.-Plant Cell Rep. 6: 94-97, 1987. Go to original source...
    32. Tang, G.X., Zhou, W.J., Li, H.Z., Mao, B.Z., He, Z.H., Yoneyama, K.: Medium, explant and genotype factors influencing shoot regeneration in oilseed Brassica spp.-J. Agron. Crop Sci. 189: 351-358, 2003. Go to original source...
    33. Yu, F.Q., Liu, H.L.: Effects of donor materials and media on microspore embryoids yield of Brassica napus.-J. Huazhong Agric. Univ. 14: 327-332, 1995.
    34. Zhang, G.Q., Tang, G.X., Song, W.J., Zhou, W.J.: Resynthesizing Brassica napus from interspecific hybridization between Brassica rapa and B. oleracea through ovary culture.-Euphytica 140: 181-187, 2004. Go to original source...
    35. Zhang, G.Q., Zhou, W.J., Gu, H.H., Song, W.J., Momoh, E.J.J.: Plant regeneration from the hybridization of Brassica napus and B. juncea through embryo culture.-J. Agron. Crop Sci. 189: 347-350, 2003. Go to original source...
    36. Zhang, Z.J., Zhou, W.J., Li, H.Z.: The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato.-Acta Physiol. Plant. 27: 363-369, 2005. Go to original source...
    37. Zhou, W.J., Hagberg, P., Tang, G.X.: Increasing embryogenesis and doubling efficiency by immediate colchicine treatment of isolated microspore in spring Brassica napus.-Euphytica 128: 27-34, 2002a. Go to original source...
    38. Zhou, W.J., Tang, G.X., Hagberg, P.: Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus.-Plant Growth Regul. 37: 185-192, 2002b. Go to original source...

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