biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:785-788, 2006 | DOI: 10.1007/s10535-006-0132-z

Comparison of induction frequency, maturation capacity and germination of Abies numidica during secondary somatic embryogenesis

B. Vooková1,*, A. Kormu»ák1
1 Institute of Plant Genetics and Biotechnology, Slovak Academy of Science, Nitra, Slovak Republic

Efficiency of the method for improving repetitive somatic embryogenesis and plant recovery of Algerian fir (Abies numidica De Lann.) was investigated by evaluating of induction frequency, maturation capacity and germination. Individual zygotic embryos differed only slightly in induction frequencies (6.8 %) from somatic embryos of the first (5.7 %) and second cycle (5.5-9.0 %). The yield of mature embryos differed significantly among the cell lines of the same cycle and among cell lines of the different cycles. Percentage of abnormalities was lowest in the first cycle of somatic embryos, whereas the second and the third cycles of somatic embryos were branded by a higher frequency of abnormalities. The differences in germination of well developed somatic embryos depended on cell lines rather than on cycle of somatic embryos.

Keywords: abscisic acid; Algerian fir; benzylaminopurine; polyethylene glycol
Subjects: Abies numidica; abscisic acid (ABA); Algerian fir; cytokinins; gibberellic acid, gibberellins; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Murashige and Skoog; nutrient medium, Schenk and Hildebrandt (SH)

Received: April 14, 2005; Accepted: August 20, 2005; Published: December 1, 2006  Show citation

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Vooková, B., & Kormu»ák, A. (2006). Comparison of induction frequency, maturation capacity and germination of Abies numidica during secondary somatic embryogenesis. Biologia plantarum50(4), 785-788. doi: 10.1007/s10535-006-0132-z
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