biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:229-234, 2007 | DOI: 10.1007/s10535-007-0046-4

Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana

J. M. Wu1, Z. G. Zhao1, H. Xing3, H. P. Guo1, W. X. Li1, L. Z. An1,2,*, S. J. Xu1, T. Chen2
1 Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, Lanzhou, P.R. China
2 Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science, Lanzhou, P.R. China
3 College of Agronomy, Gansu Agriculture University, Lanzhou, P.R. China

The influence of freezing treatment on plasma membrane (PM) H+-ATPase was investigated using plasma membrane vesicles isolated from calluses from Chorispora bungeana Fisch. & C.A. Mey. by the discontinuous sucrose gradient centrifugation. Freezing treatment (-4 °C) for 5 d resulted in significant increases in the ATPase activity and the activity of p-nitrophenyl phosphate (PNPP) hydrolysis, decreases in the Km for ATP hydrolysis and PNPP hydrolysis, and the shift of optimal pH from 6.5 to 7.0. Also, the activity PNPP hydrolysis was less sensitive to vanadate after freezing treatment compared to control, while the inhibition of ATP hydrolysis by hydroxylamine was more sensitive. In addition, freezing treatment also decreased the activation effects of trypsin on PNPP hydrolysis, but increased the activation effects of lysophosphatidylcholine on ATP hydrolysis. Taken together, these results suggested that PM H+-ATPase might play an important role during adaptation to freezing and enhancing the frost hardness in C. bungeana.

Keywords: alpine plant; kinase domain; p-nitrophenyl phosphate; phosphatase domain
Subjects: alpine plants; ATPase; Chorispora bungeana; in vitro culture, callus growth and duration; plasma membrane; temperature, low

Received: March 14, 2005; Accepted: February 25, 2006; Published: June 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Wu, J.M., Zhao, Z.G., Xing, H., Guo, H.P., Li, W.X., An, L.Z., Xu, S.J., & Chen, T. (2007). Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana. Biologia plantarum, 51(2), 229-234. doi: 10.1007/s10535-007-0046-4
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. A, Y.R., Tan, D.Y., Li, Z.J., Yao, F.: The relationship between the structures of vegetative organs in Chorispora bungeana and its environment.-J. Xinjiang Agr. Univ. 21: 273-277, 1998.
    2. Ahn, S.J, Im, Y.J., Chung, G.C., Cho, B.H.: Inducible expression of plasma membrane H+-ATPase in the roots of figleaf gourd plants under chilling root temperature.-Physiol. Plant. 106: 35-40, 1999. Go to original source...
    3. An, L.Z., Liu, Y.H., Feng, G.N., Feng, H.Y., Chen, T., Cheng, G.D.: Studies on ecological properties of altifrigetic subnival vegetation at the source area of Urumqi river.-Acta bot. Boreal-Occident. sin. 20: 98-105, 2000.
    4. Arora, R., Palta, J.P.: A loss in plasma membrane ATPase activity and its recovery coincides with incipient freeze-thaw injury and is recovery in onion bulb scale tissue.-Plant Physiol. 95: 845-852, 1991. Go to original source...
    5. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    6. Briskin, D.P.: The plasma membrane H+-ATPase of higher plant cells: biochemistry and transport function.-Biochim. biophys. Acta 1019: 95-109, 1990. Go to original source...
    7. Chen, K.-M., Gong, H.-J., Wang, S.-M., Zheng, W.-J., Zhang, L.-C.: Regulation of the structure and catalytic properties of plasma membrane H+-ATPase involved in adaptation of two reed ecotypes to their different habitats.-Biol. Plant. 49: 513-519, 2005. Go to original source...
    8. Cooke, D.T., Burden, R.S.: Lipid modulation of plasma membrane-bound ATPase.-Physiol. Plant. 78: 153-159, 1990. Go to original source...
    9. Dell'Orto, M., Santi, S., Nisi, P.D., Cesco, S., Varanini, Z., Zocchi, G., Pinton, R.: Development of Fe-deficiency responses in cucumber (Cucumis sativus L.) roots: involvement of plasma membrane H+-ATPase activity.-J. exp. Bot. 51: 695-701, 2000. Go to original source...
    10. Dong, C.Z., Montillet, J.L., Triantaphylides, C.: Effect of gamma irradiation on the plasma membrane of suspension-cultured apple cells: Rapid irreversible inhibition of H+-ATPase activity.-Physiol. Plant. 90: 307-312, 1994. Go to original source...
    11. Fu, X.Y., Chang, J.F., An, L.Z., Zhang, M.X., Xu, S.J., Chen, T., Liu, Y.H., Xing, H., Wang, J.H.: Association of the cold-hardness of Chorispora bungeana with the distribution and accumulation of calcium in the cells and tissues.-Environ. exp. Bot. 55: 282-293, 2006. Go to original source...
    12. Gong, H.J., Chen, K.M., Chen, G.C., Wang, S.M., Zhang, C.L.: Drought stress stimulates p-nitrophenyl phosphate hydrolysis rate of the plasma membrane H+-ATPase from wheat leaves.-Plant Growth Regul. 40: 139-145, 2003. Go to original source...
    13. Hellergren, J., Widell, S., Lundborg, T., Kylin, A.: Frost hardiness development in Pinus sylvestris: the involvement of a K+-stimulated Mg2+-dependent ATPase from purified plasma membranes of pine.-Physiol. Plant. 58: 7-12, 1983. Go to original source...
    14. Iswari, S., Palta, J.P.: Plasma membrane ATPase activity following reversible and irreversible freezing injury.-Plant Physiol. 90: 1088-1095, 1989. Go to original source...
    15. Michelet, B., Boutry, M.: The plasma membrane H+-ATPase: a highly regulated enzyme with multiple physiological functions.-Plant Physiol. 108: 1-6, 1995. Go to original source...
    16. Morandini, P., Valera, M., Albumi, C., Bonza, M.C., Giacometti, J.M.S., Ravera, G., Murgia, I., Soave, C., Michelis, M.: A novel interaction partner for the C-terminus of Arabidopsis thaliana plasma membrane H+-ATPase (AHA1 isoform): site and mechanism of action on H+-ATPase activity differ from those of 14-3-3 proteins.-Plant J. 31: 487-497, 2002. Go to original source...
    17. Morsomme, P., Boutry, M.: The plant plasma membrane H+-ATPase: structure, function and regulation.-Biochim. biophys. Acta 1465: 1-16, 2000. Go to original source...
    18. Palmgren, M.G.: Regulation of plant plasma membrane H+-ATPase activity.-Physiol. Plant. 83: 314-323, 1991. Go to original source...
    19. Palmgren, M.G., Larsson, C., Sommarin, M.: Proteolytic activation of the plant plasma membrane H+-ATPase by removal of a terminal segment.-J. biol. Chem. 265: 13423-13426, 1990. Go to original source...
    20. Palmgren, M.G., Sommarin, M., Ulvskov, P., Jorgensen, P.L.: Modulation of plasma membrane H+-ATPase from oat roots by lysophosphatidylcholine, free fatty acids and phospholipase A2.-Physiol. Plant. 74: 11-19, 1988. Go to original source...
    21. Qiu, Q.S.: Characterization of PNPP hydrolysis by plasma membrane H+-ATPases from soybean hypocotyls.-J. Plant Physiol. 154: 628-633, 1999. Go to original source...
    22. Qiu, Q.S., Su, X.F.: The influence of extracellular-side Ca2+ on the activity of the plasma membrane H+-ATPase from wheat roots.-Aust. J. Plant Physiol. 25: 923-928, 1998. Go to original source...
    23. Qiu, Q.S., Zhang, N.: Water stress inhibits p-nitrophenyl phosphate hydrolysis activity of the plasma membrane H+-ATPase from soybean hypocotyls.-Aust. J. Plant Physiol. 27: 717-721, 2000. Go to original source...
    24. Qiu, Q.S., Zhang, N.: Effect of lysophosphatidylcholine on ATP and p-nitrophenyl phosphate hydrolysis by the plasma membrane H+-ATPase from soybean hypocotyls.-Acta bot. sin. 43: 1140-1145, 2001.
    25. Rodriguez-Rosales, M.P., Kerkeb, L., Bueno, P., Donaire, J.P.: Changes induced by NaCl in lipid content and composition, lipoxygenase, plasma membrane H+-ATPase and antioxidant enzyme activities of tomato (Lycopersicon esculentum Mill.) calli.-Plant Sci. 143: 143-150, 1999. Go to original source...
    26. Serrano, R.: Structure and function of plasma membrane ATPase.-Annu. Rev. Plant Physiol. Plant mol. Biol. 40: 61-94, 1989. Go to original source...
    27. Steponkus, P.L.: Role of the plasma membrane in freezing injury and cold acclimation.-Annu. Rev. Plant Physiol. 35: 543-586, 1984. Go to original source...
    28. Suwastika, I.N., Gehring, C.A.: The plasma membrane H+-ATPase from Tradescantia stem and leaf tissue is modulated in vitro by cGMP.-Arch. Biochem. Biophys. 367: 137-139, 1999. Go to original source...
    29. Vara, F., Serrano, R.: Phosphorylated intermediate of the ATPase of plant plasma membranes.-J. biol. Chem. 258: 5334-5336, 1983. Go to original source...
    30. Widell, S., Larsson, C.: A critical evaluation of markers used in plasma membrane purification.-In: Larsson, C., Moller, I.M.: The Plant Plasma Membrane. Pp. 16-43. Springer-Verlag, Berlin 1990. Go to original source...
    31. Yan, F., Zhu, Y.Y., Muller, C., Zorb, C., Schubert, S.: Adaptation of H+-pumping and plasma membrane H+-ATPase activity in proteoid roots of white lupin under phosphate deficiency.-Plant Physiol. 129: 50-63, 2002. Go to original source...
    32. Yang, Y.L., Guo, J.K., Zhang, F., Zhao, L.Q., Zhang, L.X.: NaCl induced changes of the H+-ATPase in root plasma membrane of two wheat cultivars.-Plant Sci. 166: 913-918, 2004. Go to original source...
    33. Zhang, N., Qiu, Q.S.: The effect of trypsin treatment on plasma membrane H+-ATPase from soybean hypocotyls.-J. Beijing normal Univ. 36: 390-393, 2000.
    34. Zhao, L.Q., Zhang, F., Guo, J.K., Yang, Y.L., Li, B.B., Zhang, L.X.: Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed.-Plant Physiol. 134: 849-857, 2004. Go to original source...
    35. Zhao, S., Colombo, S.J., Blumwald, E.: The induction of freezing tolerance in jack pine seedling: The role of root plasma membrane H+-ATPase and redox activities.-Physiol. Plant. 93: 55-60, 1995. Go to original source...

    Return to the content