biologia plantarum

Biologia plantarum 52:87-92, 2008 | DOI: 10.1007/s10535-008-0014-7

Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale

Q. Liu¹, J. L. Yang¹, L. S. He¹, Y. Y. Li¹, S. J. Zheng^2,*

¹ College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, P.R. China

² State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, P.R. China

Two triticale cultivars ZC 237 (Al-resistant) and ZC 1890 (Al-sensitive) were used to investigate the effects of 30 to 100 µM Al on antioxidative enzyme activity, lipid peroxidation and cell wall composition. In ZC 1890, the root elongation was significantly inhibited after 1-h exposure to 50 µM Al, the changes in hemicellulose fraction were clearly detected after 2-h Al exposure, while the peroxidase (POD) and superoxide dismutase (SOD) activities significantly increased after 6-h exposure, and the malondialdehyde (MDA) content after 12-h exposure. The similar patterns were also found in ZC 237. Treatment of ZC 1890 with 1 mM citrate for 30 min after 3-h exposure to Al resulted in significant decrease of Al bound to cell-wall and recovery of root elongation. These results suggested that Al affected cell wall before the damage of plasma membrane, but this was not the primary cause of root elongation inhibition.

Keywords: lipid peroxidation; oxidative stress; toxicity; Triticosecale

Subjects: antioxidants, antioxidant enzymes; heavy metals; in vitro culture, cell suspension; in vitro culture, rooting; lipid peroxidation; malondialdehyde; peroxidase; plasma membrane; thiobarbituric acid; Triticosecale

Received: January 17, 2006; Accepted: September 3, 2006; Published: March 1, 2008  Show citation