biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:237-241, 2008 | DOI: 10.1007/s10535-008-0052-1

Relationships among Leymus species assessed by RAPD markers

R. W. Yang1,2, Y. H. Zhou2,*, C. B. Ding1, Y. L. Zheng2, L. Zhang1
1 College of Biology and Science, Sichuan Agricultural University, Yaan, Sichuan, P.R. China
2 Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, P.R. China

The DNA genetic diversity of 40 accessions of genus Leymus was analyzed by random amplified polymorphic DNA (RAPD) markers. A total of 352 products were amplified by 34 10-mer arbitrary primers, among which 337 products (95.74 %) were found to be polymorphic. 5-14 polymorphic bands were amplified by each polymorphic primer, with an average of 9.91 bands. The data of 352 RAPD bands were used to generate Jaccard's similarity coefficients and to construct a dendrogram by means of UPGMA. Great genetic diversity in genus Leymus was observed, the genetic diversity among the different species more abundant than that of the different accessions, and the different accessions in a species or the species from the same areas were clustered together.

Keywords: cluster analysis; genetic diversity; molecular marker; polymerase chain reaction; similarity coefficients
Subjects: DNA markers; genetic diversity, biodiversity; Leymus sp.; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD)

Received: November 13, 2006; Accepted: April 5, 2007; Published: June 1, 2008  Show citation

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Yang, R.W., Zhou, Y.H., Ding, C.B., Zheng, Y.L., & Zhang, L. (2008). Relationships among Leymus species assessed by RAPD markers. Biologia plantarum52(2), 237-241. doi: 10.1007/s10535-008-0052-1
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