biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:312-316, 2009 | DOI: 10.1007/s10535-009-0056-5

High frequency plant regeneration from the cotyledonary node of common bean

W. Dang1,2, Z. M. Wei1,*
1 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China
2 Graduate School of the Chinese Academy of Sciences, Shanghai, P.R. China

An efficient regeneration system for Phaseolus vulgaris was developed from mature seeds germinated on Murashige and Skoog (MS) medium supplemented with thidiazuron or N6-benzylaminopurine (BA) for 6 d. Using cotyledonary nodes, multiple buds were induced on the MS medium supplemented with 5.0 mg dm-3 BA with the induction frequency 71.9 % after 4-week culture. The buds were then transferred onto shoot formation medium containing 1.0 mg dm-3 BA, 0.1 mg dm-3 gibberellic acid and 2.0 mg dm-3 silver nitrate. The addition of AgNO3 enhanced the frequency of the shoot formation from 61.3 to 87.6 %. Root induction medium was half-strength MS medium with 0.75 mg dm-3 indolebutyric acid and 0.02 mg dm-3 BA. The average root frequency was 84.3 %. The regenerated plantlets with healthy roots grew successfully when transferred to soil. Using this system we obtained over 10 regenerated plantlets from one explant.

Keywords: bud induction; Phaseolus vulgaris; shoot development; silver nitrate; thidiazuron
Subjects: auxins; common bean; cytokinins; ex vitro transfer; gibberellic acid, gibberellin; in vitro culture, direct regeneration; in vitro culture, micropropagation; in vitro culture, rooting; Phaseolus vulgaris; silver nitrate

Received: March 8, 2007; Accepted: January 25, 2008; Published: June 1, 2009  Show citation

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Dang, W., & Wei, Z.M. (2009). High frequency plant regeneration from the cotyledonary node of common bean. Biologia plantarum, 53(2), 312-316. doi: 10.1007/s10535-009-0056-5
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