biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 54:477-482, 2010 | DOI: 10.1007/s10535-010-0084-1

Endogenous brassinosteroids in wheat treated with 24-epibrassinolide

A. Janeczko1,*, J. Swaczynová2
1 Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland
2 Laboratory of Growth Regulators, Palacký University, Olomouc, Czech Republic

The aim of the study was to examine the effect of exogenous 24-epibrassinolide on its uptake and content of endogenous brassinosteroids in wheat seedlings. 24-Epibrassinolide was applied at two concentrations (0.1 and 2.0 μM) and in three different methods: by soaking seeds, by drenching and by spraying plants. Brassinosteroids were determined by high-performance liquid chromatography combined with electrospray mass spectrometry. Three important brassinosteroids, 24-epibrassinolide, brassinolide and castasterone, were detected in the wheat leaves, but their contents varied with leaf insertion and plant age. Increased 24-epibrassinolide content in the leaf tissue was found when this hormone was applied by soaking or drenching. Additionally the seed treatment influenced brassinosteroid balance in seedlings. The growth response of wheat seedlings treated with 24-epibrassinolide has been also investigated.

Keywords: brassinolide; castasterone; growth response; homobrassinolide; HPLC, MS
Subjects: brassinosteroids; growth and growth analysis; Triticum aestivum; wheat

Received: June 11, 2008; Accepted: March 16, 2009; Published: September 1, 2010  Show citation

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Janeczko, A., & Swaczynová, J. (2010). Endogenous brassinosteroids in wheat treated with 24-epibrassinolide. Biologia plantarum, 54(3), 477-482. doi: 10.1007/s10535-010-0084-1
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