biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:614-620, 2010 | DOI: 10.1007/s10535-010-0111-2

Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress

W. -H. Sun1, M. Duan1, F. Li1, D. -F. Shu1, S. Yang1, Q. -W. Meng1,*
1 College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, P.R. China

In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco. Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtAPX in tobacco improved seed germination under temperature stress. Two transgenic tobacco lines showed higher ascorbate peroxidase activity, accumulated less hydrogen peroxide and malondialdehyde than wild type plants under stress condition. The photochemical efficiency of photosystem 2 in the transgenic lines was distinctly higher than that of wild type plants under chilling and heat stresses. Results indicated that the over-expression of TtAPX enhanced tolerance to temperature stress in transgenic tobacco plants.

Keywords: Lycopersicum esculentum; malondialdehyde; Nicotiana tabacum; photosystem 2; thylakoid-bound ascorbate peroxidase; transgenic tobacco
Subjects: Agrobacterium tumefaciens; ascorbate peroxidase; chlorophyll fluorescence; electrolyte leakage; hydrogen peroxide; Lycopersicon esculentum; malondialdehyde; photosynthetic rate; superoxide dismutase (SOD); temperature high; temperature low; tomato; transgenic plants

Received: March 17, 2009; Accepted: September 25, 2009; Published: December 1, 2010  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Sun, W.-H., Duan, M., Li, F., Shu, D.-F., Yang, S., & Meng, Q.-W. (2010). Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress. Biologia plantarum, 54(4), 614-620. doi: 10.1007/s10535-010-0111-2
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Asada, K.: Ascorbate peroxidase - a hydrogen peroxidescavenging enzyme in plants. - Physiol. Plant. 85: 235-241, 1992. Go to original source...
    2. Asada, K.: The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. - Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 601-639, 1999. Go to original source...
    3. Bhattacharjee, S.: Calcium-dependent signaling pathway in the heat-induced oxidative injury in Amaranthus lividus. - Biol. Plant. 52: 137-140, 2008. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Chen, G.X., Asada, K.: Ascorbate peroxidases in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. - Plant Cell Physiol. 30: 987-998, 1989.
    6. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutases. I. Occurrence in higher plants. - Plant Physiol. 59: 309-314, 1977. Go to original source...
    7. Kavitha, K., Venkataraman, G., Parida, A.: An oxidative and salinity stress induced peroxisomal ascorbate peroxidase from Avicennia marina: molecular and functional characterization. - Plant Physiol. Biochem. 46: 794-804, 2008. Go to original source...
    8. Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. - Photosynth. Res. 25: 147-150, 1990. Go to original source...
    9. Kratsch, H.A., Wise, R.R.: The ultrastructure of chilling stress. - Plant Cell Environ. 23: 337-350, 2000. Go to original source...
    10. Lee, Y.P., Kim, S.H., Bang, J.W., Lee, H.S., Kwak, S.S., Kwon, S.Y.: Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. - Plant Cell Rep. 26: 591-598, 2007. Go to original source...
    11. Murgia, I., Tarantino, D., Vannini, C., Bracale, M., Carravieri, S., Soave, C.: Arabidopsis thaliana plants over-expressing thylakoidal ascorbate peroxidase show increased resistance to paraquat-induced photo-oxidative stress and to nitric oxide-induced cell death. - Plant J. 38: 940-953, 2004. Go to original source...
    12. Robinson, S.P., Downton, W.J.S., Millhouse, J.A.: Photosynthesis and ion content of leaves and isolated chloroplasts of salt-stressed spinach. - Plant Physiol. 73: 238-242, 1983. Go to original source...
    13. Sairam, P.K., Srivastava, G.C.: Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. - Plant Sci. 162: 897-904, 2002. Go to original source...
    14. Shi, W.M., Muramoto, Y., Ueda, A., Takabe, T.: Cloning of peroximal ascorbate peroxidase gene from barley and enhanced thermo-tolerance by over-expressing in Arabidopsis thaliana. - Gene 273: 23-27, 2001. Go to original source...
    15. Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K.: Regulation and function of ascorbate peroxidase isoenzymes. - J. exp. Bot. 53: 1305-1309, 2002. Go to original source...
    16. Song, X.S., Hu, W.H., Mao, W.H., Ogweno, J.O., Zhou, Y.H., Yu, J.Q.: Response of ascorbate peroxidase isoenzymes and ascorbate regeneration system to abiotic stresses in Cucumis sativus L. - Plant Physiol. Biochem. 43: 1082-1088, 2005. Go to original source...
    17. Wang, W.Q., Li, B., Meng, Q.W., Zou, Q.: The sequence of Lycopersicon esculentum thylakoid-bound ascorbate peroxidase gene TtAPX. - Plant Physiol. mol. Biol. 28: 491-492, 2002. Go to original source...
    18. Wang, Y., Yang, Z.M., Zhang, Q.F., Li, J.L.: Enhanced chilling tolerance in Zoysia matrella by pre-treatment with salicylic acid, calcium chloride, hydrogen peroxide or 6-benzylaminopurine. - Biol. Plant. 53: 179-182, 2009. Go to original source...
    19. Yabuta, Y., Motoki, T., Yoshimura, K., Takeda, T., Ishikawa, T., Shigeoka, S.: Thylakoid membrane-bound peroxidase is a limiting factor of anti-oxidative systems under photo-oxidative stress. - Plant J. 32: 915-925, 2002. Go to original source...
    20. Yoshimura, K., Yabuta, Y., Ishikawa, T., Shigeoka, S.: Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. - Plant Physiol. 123: 223-233, 2000. Go to original source...
    21. Zhou, Y.H., Yu, J.Q., Huang, L.F., Nogues, S.: The relationship between CO2 assimilation, photosynthetic electron transport and water-water cycle in chilling-exposed cucumber leaves under low light and subsequent recovery. - Plant Cell Environ. 27: 1503-1514, 2004. Go to original source...

    Return to the content