biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:243-252, 2011 | DOI: 10.1007/s10535-011-0035-5

Determination of floral initiation in Malus domestica: a novel morphogenetic approach

M. R. Dadpour1,3,*, A. Movafeghi2,3, W. Grigorian1, Y. Omidi3
1 Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Department of Plant Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
3 Research Centre for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran

Floral initiation in apple (Malus domestica Borkh) was studied by a novel morphogenetic approach. Developmental stages of apices were evaluated based on the morphology of shoot apical meristem (SAM) from various collection dates. Besides, the frequency of each stage was calculated within apices populations after full blooming (DAFB). Prior to doming of apex, three marked phases were found based on SAM morphology: 1) narrow appearance (vegetative phase), 2) broadened form (transition phase), and 3) prominent shape (commitment phase). A furrow region was formed at the base of leaf primordium during the bract initiation, while significant broadening of SAM was observed. Cell division patterns manifested in modification of anisotropic clusters from isotropic cellular packets, as a result of which profound morphological changes of apices occurred. Based on these findings, we propose that the structural alterations prior to doming may be taken into account for determination of the initial development and reproduction signs in apple trees.

Keywords: apple; cell division pattern; epi-illumination light microscopy; fluorescence microscopy; shoot apical meristem

Received: June 2, 2009; Accepted: May 17, 2010; Published: June 1, 2011  Show citation

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Dadpour, M.R., Movafeghi, A., Grigorian, W., & Omidi, Y. (2011). Determination of floral initiation in Malus domestica: a novel morphogenetic approach. Biologia plantarum55(2), 243-252. doi: 10.1007/s10535-011-0035-5
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References

  1. Albrechtova, J.T.P., Dueggelin, M., Duerrenberger, M., Wagner, E.: Changes in the geometry of the apical meristem and concomitant changes in cell wall properties during photoperiodic induction of flowering in Chenopodium rubrum. - New Phytol. 163: 263-269, 2004. Go to original source...
  2. Banno, K., Hayashi, S., Tanabe, K.: Relationships between flower bud formation and endogenous growth regulators in Japanese pear cultivars (Pyrus serotina Rehd). - J. jap. Soc. hort. Sci. 54: 15-25, 1985. Go to original source...
  3. Bernier, G.: Growth changes in the shoot apex of Sinapis alba during transition to flowering. - J. exp. Bot. 48: 1071-1077, 1997. Go to original source...
  4. Buban, T., Faust, M.: Flower bud induction in apple trees: internal control and differentiation. - Hort. Rev. 4: 174-203, 1982. Go to original source...
  5. Charlton, W.A., MacDonald, A.D., Posluszny, U., Wilkins, C.P.: Additions to the technique of epi-illumination light microscopy for the study of floral and vegetative apices. - Can. J. Bot. 67: 1739-1743, 1989. Go to original source...
  6. Clark, S.E.: Organ formation at the vegetative shoot meristem. - Plant Cell 9: 1067-1076, 1997. Go to original source...
  7. Coen, E., Rolland-Lagan, A.G., Matthews, M., Bangham, J.A., Prusinkiewicz, P.: The genetics of geometry. - Proc. nat. Acad. Sci. USA 101: 4728-4735, 2004. Go to original source...
  8. Dencker, I., Hansen, P.: Flower initiation and node development of axillary buds, growth partitioning, and bleeding sap production of young apple-trees as affected by fertigation. - J. hort. Sci. 69: 869-876, 1994. Go to original source...
  9. Diaz, D.H., Rasmussen, H.P., Dennis, J.F.G.: Scanning electron microscope examination of flower bud differentiation in sour cherry. - J. amer. Soc. hort. Sci. 106: 513-515, 1981. Go to original source...
  10. Dumais, J., Kwiatkowska, D.: Analysis of surface growth in shoot apices. - Plant J. 31: 229-241, 2002. Go to original source...
  11. Evans, R.C., Dickinson, T.A.: Floral ontogeny and morphology in subfamily Spiraeoideae Endl. (Rosaceae). - Int. J. Plant Sci. 160: 981-1012, 1999. Go to original source...
  12. Evans, R.C., Dickinson, T.A.: Floral ontogeny and morphology in Gillenia (Spiraeoideae) and subfamily Maloideae C. Weber (Rosaceae). - Int. J. Plant Sci. 166: 427-447, 2005. Go to original source...
  13. Fleming, A.J.: The co-ordination of cell division, differentiation and morphogenesis in the shoot apical meristem: a perspective. - J. exp. Bot. 57: 25-32, 2006. Go to original source...
  14. Foster, T., Johnston, R., Seleznyova, A.: A morphological and quantitative characterization of early floral development in apple (Malus × domestica Borkh.). - Ann. Bot. 92: 199-206, 2003. Go to original source...
  15. Grandjean, O., Vernoux, T., Laufs, P., Belcram, K., Mizukami, Y., Traas, J.: In vivo analysis of cell division, cell growth, and differentiation at the shoot apical meristem in Arabidopsis. - Plant Cell 16: 74-87, 2004. Go to original source...
  16. Gross-Hardt, R., Laux, T.: Stem cell regulation in the shoot meristem. - J. cell. Sci. 116: 1659-1666, 2003. Go to original source...
  17. Guimond, C.M., Lang, G.A., Andrews, P.K.: Timing and severity of summer pruning affects flower initiation and shoot regrowth in sweet cherry. - Hort. Sci. 33: 647-649, 1998. Go to original source...
  18. Hirst, P.M., Ferree, D.C.: Rootstock effects on the flowering of delicious apple. 1. Bud development. - J. amer. Soc. hort. Sci. 120: 1010-1017, 1995. Go to original source...
  19. Hirst, P.M., Ferree, D.C.: Effects of rootstock on bud development and flower formation of 'starkspur supreme delicious' apple. - Fruit Varieties J. 50: 25-34, 1996.
  20. Hoover, E., De Silva, N., Mcartney, S., Hirst, P.: Bud development and floral morphogenesis in four apple cultivars. - J. hort. Sci. Biotechnol. 79: 981-984, 2004. Go to original source...
  21. Huang, H., Qiao, X.S., Cao, S.Y.: Studies on the period of physiological differentiation of flower bud in apple trees. - Acta hort. sin. 13: 181-186, 1986.
  22. Korn, R.W.: The changing shape of plant cells: transformations during cell proliferation. - Ann. Bot. 46: 649-666, 1980. Go to original source...
  23. Kwiatkowska, D.: Surface growth at the reproductive shoot apex of Arabidopsis thaliana pin-formed 1 and wild type. - J. exp. Bot. 55: 1021-1032, 2004. Go to original source...
  24. Kwiatkowska, D.: Flower primordium formation at the Arabidopsis shoot apex: quantitative analysis of surface geometry and growth. - J. exp. Bot. 57: 571-580, 2006. Go to original source...
  25. Kwiatkowska, D.: Flowering and apical meristem growth dynamics. - J. exp. Bot. 59: 187-201, 2008. Go to original source...
  26. Kwiatkowska, D., Dumais, J.: Growth and morphogenesis at the vegetative shoot apex of Anagallis arvensis L. - J. exp. Bot. 54: 1585-1595, 2003. Go to original source...
  27. Lamp, B.M., Connell, J.H., Duncan, R.A., Viveros, M., Polito, V.S.: Almond flower development: floral initiation and organogenesis. - J. amer. Soc. hort. Sci. 126: 689-696, 2001. Go to original source...
  28. Laux, T., Mayer, K.F., Berger, J., Jurgens, G.: The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis. - Development 122: 87-96, 1996. Go to original source...
  29. Lemon, G.D., Posluszny, U.: A new approach to the study of apical meristem development using laser scanning confocal microscopy. - Can. J. Bot. 76: 899-904, 1998. Go to original source...
  30. Luckwill, L.C., Silva, J.M.: Effects of daminozide and gibberellic-acid on flower initiation, growth and fruiting apple cv Golden delicious. - J. hort. Sci. 54: 217-223, 1979. Go to original source...
  31. Muller, R., Borghi, L., Kwiatkowska, D., Laufs, P., Simon, R.: Dynamic and compensatory responses of Arabidopsis shoot and floral meristems to CLV3 signaling. - Plant Cell 18: 1188-1198, 2006. Go to original source...
  32. Oukabli, A., Bartolin, S., Viti, R.: Anatomical and morphological study of apple (Malus × domestica Borkh.) flower buds growing under inadequate winter chilling. - J. hort. Sci. Biotechnol. 78: 580-585, 2003. Go to original source...
  33. Posluszny, U., Scott, M.G., Sattler, R.: Revisions in the technique of epi-illumination light microscopy for the study of floral and vegetative apices. - Can. J. Bot. 58: 2491-2495, 1980. Go to original source...
  34. Pratt, C., Einset, J., Zahur, M.: Radiation damage in apple shoot apices. - Amer. J. Bot. 46: 537-544, 1959. Go to original source...
  35. Pratt, C., Einset, J., Zahur, M.: Apple flower and fruit: morphology and anatomy. - Hort. Rev. 10: 273-308, 1988. Go to original source...
  36. Reddy, G.V., Meyerowitz, E.M.: Stem-cell homeostasis and growth dynamics can be uncoupled in the Arabidopsis shoot apex. - Science 310: 663-667, 2005. Go to original source...
  37. Rolinson, A.E.: Rates of cell division in the vegetative shoot apex of rice (Oryza sativa L.). - Ann. Bot. 40: 939-945, 1976. Go to original source...
  38. Ryugo, K.: Promotion and inhibition of flower initiation and fruit set by plant manipulation and hormones. - Acta hort. 179: 301-307, 1986. Go to original source...
  39. Sharma, V.K., Carles, C., Fletcher, J.C.: Maintenance of stem cell populations in plants. - PNAS 100: 11823-11829, 2003. Go to original source...
  40. Sharma, V.K., Fletcher, J.C.: Maintenance of shoot and floral meristem cell proliferation and fate. - Plant Physiol. 129: 31-39, 2002. Go to original source...
  41. Tooke, F., Battey, N.: Models of shoot apical meristem function. - New Phytol. 159: 37-52, 2003. Go to original source...
  42. Tromp, J.: Flower-bud formation and shoot growth in apple as affected by temperature. - Scientia Hort. 5: 331-338, 1976. Go to original source...
  43. Veit, B.: Determination of cell fate in apical meristems. - Curr. Opin. Plant Biol. 7: 57-64, 2004. Go to original source...
  44. Wang, A.X., Wang, D.Y.: Regulation of the ALBINO3-mediated transition to flowering in Arabidopsis depends on the expression of CO and GA1. - Biol. Plant. 53: 484-492, 2009. Go to original source...