biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:89-96, 2012 | DOI: 10.1007/s10535-012-0021-6

Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips

F. J. Xu1, G. Li2, C. W. Jin1, W. J. Liu3, S. S. Zhang3, Y. S. Zhang3, X. Y. Lin1,*
1 MoE Key Laboratory of Environmental Remediation and Ecosystem Health, Hangzhou, P.R. China
2 Institute of Urban Environment, Chinese Academy of Sciences, Fujian, P.R. China
3 College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, P.R. China

The present study investigated the effects of aluminum on lipid peroxidation, accumulation of reactive oxygen species and antioxidative defense systems in root tips of wheat (Triticum aestivum L.) seedlings. Exposure to 30 μM Al increased contents of malondialdehyde, H2O2, suproxide radical and Evans blue uptake in both genotypes, with increases being greater in Al-sensitive genotype Yangmai-5 than in Al-tolerant genotype Jian-864. In addition, Al treatment increased the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), glutathione reductase (GR) and glutathione peroxidase (GPX), as well as the contents of ascorbate (AsA) and glutathione (GSH) in both genotypes. The increased activities SOD and POD were greater in Yangmai-5 than in Jian-864, whereas the opposite was true for the activities of CAT, APX, MDHAR, GR and GPX and the contents of AsA and GSH. Consequently, the antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging activity and ferric reducing/antioxidant power (FRAP) was greater in Jian-864 than in Yangmai-5.

Keywords: aluminum toxicity; APX; CAT; DPPH; FRAP; GPS; GR; oxidative stress; POD; SOD; Triticum aestivum
Subjects: aluminum toxicity; oxidative stress; aluminum; ascorbate; ascorbate peroxidase; catalase; glutathione peroxidase; glatathione reductase; glutathione; malondialdehyde; programmed cell death; superoxide dismutase; hydrogen peroxide; ROS

Received: June 17, 2010; Accepted: September 23, 2010; Published: March 1, 2012  Show citation

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Xu, F.J., Li, G., Jin, C.W., Liu, W.J., Zhang, S.S., Zhang, Y.S., & Lin, X.Y. (2012). Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips. Biologia plantarum, 56(1), 89-96. doi: 10.1007/s10535-012-0021-6
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