biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:516-520, 2012 | DOI: 10.1007/s10535-012-0075-5

Over-expressing GsGST14 from Glycine soja enhances alkaline tolerance of transgenic Medicago sativa

Z. -Y. Wang1,2, F. -B. Song1,2, H. Cai3, Y. -M. Zhu3,*, X. Bai3, W. Ji3, Y. Li3, Y. Hua3
1 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, P.R. China
2 Graduate University of Chinese Academy of Sciences, Beijing, P.R. China
3 Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin, P.R. China

Glutathione-S-transferases (GSTs) are ubiquitous enzymes that play a key role in stress tolerance and cellular detoxification. The GST gene GsGST14 selected from the gene expression profiles of Glycine soja under alkaline stress was transformed into alfalfa (Medicago sativa L.). Transgenic alfalfa plants showed 1.73-1.99 times higher GST activity than wild-type plants. Transgenic alfalfa grew well in the presence of 100 mM NaHCO3, while wild-type plants exhibited chlorosis and stunted growth, even death. There were marked changes in malondialdehyde content and relative membrane permeability caused by alkaline stress in non-transgenic lines compared to transgenic lines. The results indicate that the gene GsGST14 could enhance alkaline resistance in transgenic alfalfa.

Keywords: glutathione S-transferase; NaHCO3; transgenic alfalfa; wild soybean
Subjects: glutathione S-transferase; alkaline stress; amino acid sequence; gene construct; transgenic plants; PCR; chlorophyll; superoxide dismutase; malondialdehyde; electrolyte leakage

Received: April 17, 2011; Accepted: July 26, 2011; Published: September 1, 2012  Show citation

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Wang, Z.-Y., Song, F.-B., Cai, H., Zhu, Y.-M., Bai, X., Ji, W., Li, Y., & Hua, Y. (2012). Over-expressing GsGST14 from Glycine soja enhances alkaline tolerance of transgenic Medicago sativa. Biologia plantarum, 56(3), 516-520. doi: 10.1007/s10535-012-0075-5
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