biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:159-163, 2013 | DOI: 10.1007/s10535-012-0235-7

Water deficit and recovery response of Medicago truncatula plants expressing the ELIP-like DSP22

S. S. Araújo1,2,*, A. S. Duque1, J. M. Silva3,4, D. Santos2, A. B. Silva3,4, P. Fevereiro1,3
1 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
2 Faculdade de Medicina Veterinária, Instituto de Investigação Científica e Tropical, Lisboa, Portugal
3 Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal
4 BioFIG, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal

In this article, we present the response of Medicago truncatula Gaert. cv. Jemalong plants expressing constitutively the Dsp22 gene from Craterostigma plantagineum to water stress and rehydration. The Dsp22 gene encodes an ELIP-like protein thought to protect the chloroplast against photooxidative damage during the dehydration and rehydration. The Dsp22 transgenic homozygous M. truncatula plants showed higher amount of chlorophyll (Chl), lower Chl a/Chl b ratio and higher actual efficiency of energy conversion in photosystem 2 (ΦPSII) after rehydration, when compared to the wild type. The combined data from the Chl a fluorescence analysis, pigment quantification and biomass accumulation showed that transgenic M. truncatula plants are able to recover from water deprivation better than wild type plants.

Keywords: abiotic stress; barrel medic; chlorophyll content; chlorophyll fluorescence; relative water content; transgenic plants
Subjects: water stress; water stress recovery; chlorophyll content; chlorophyll fluorescence; relative water content; transgenic plants

Received: March 24, 2011; Accepted: January 30, 2012; Published: March 1, 2013  Show citation

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Araújo, S.S., Duque, A.S., Silva, J.M., Santos, D., Silva, A.B., & Fevereiro, P. (2013). Water deficit and recovery response of Medicago truncatula plants expressing the ELIP-like DSP22. Biologia plantarum, 57(1), 159-163. doi: 10.1007/s10535-012-0235-7
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