biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 57:305-312, 2013 | DOI: 10.1007/s10535-012-0290-0

Patterns of protein expression in water-stressed wheat chloroplasts

A. H. M. Kamal1,2, K. Cho3, J. -S. Choi4,5, Y. Jin1, C. -S. Park6, J. S. Lee7, S. H. Woo1,*
1 Dept. of Crop Science, Chungbuk National University, Cheong-ju, Korea
2 Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
3 Division of Mass Spectrometry Research, Korea Basic Science Institute, Ochang, Chungbuk, Korea
4 Division of Life Science, Korea Basic Science Institute, Daejeon, Korea
5 Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea
6 Dept. of Crop Agriculture and Life Science, Chonbuk National University, Jeonju, Korea
7 Dept. of Biology Education, Chungbuk National University, Cheong-ju, Korea

The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.

Keywords: chloride carrier, channel family; H+-ATPase; MALDI-TOF, TOF-MS; Triticum aestivum
Subjects: protein pattern; water stress; wheat; chloroplast; chloride carrier; channel proteins; ATPase; MALDI-TOF; Rubisco

Received: September 11, 2011; Accepted: July 16, 2012; Published: June 1, 2013  Show citation

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Kamal, A.H.M., Cho, K., Choi, J.-S., Jin, Y., Park, C.-S., Lee, J.S., & Woo, S.H. (2013). Patterns of protein expression in water-stressed wheat chloroplasts. Biologia plantarum, 57(2), 305-312. doi: 10.1007/s10535-012-0290-0
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    References

    1. Abbasi, F.M., Komatsu, S.: A proteomic approach to analyse salt-responsive proteins in rice leaf sheath. - Proteomics 4: 2072-2081, 2004. Go to original source...
    2. Alam, I., Sharmin, S.A., Kim, K. H., Yang, J.K., Choi, M.S., Lee, B.H.: Proteome analysis of soybean roots subjected to short-term drought stress. - Plant Soil 333: 491-505, 2010. Go to original source...
    3. Ali, G.M., Komatsu, S.: Proteomic analysis of rice leaf sheath during drought stress. - J. Proteome Res. 5: 396-403, 2006. Go to original source...
    4. Barrs, H.D., Weatherley, P.E.: A re-examination of the relative turgidity technique for estimating water deficits in leaves. - Aust. J. biol. Sci. 15: 413-425, 1962. Go to original source...
    5. Bates, L., Waldren, R.P., Tear, I.P.: Rapid determination of free proline for water stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    6. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    7. Chaves, M.M.: Effects of water deficits on carbon assimilation. - J. exp. Bot. 42: 1-16. 1991. Go to original source...
    8. Chaves, M.M., Oliveira, M.M.: Mechanisms underlying plant resilience to water deficits, prospects for water-saving agriculture. - J. exp. Bot. 55: 2365-2384, 2004. Go to original source...
    9. Chaves, M.M., Pereira, J.S., Maroco, J.: Understanding plant response to drought - from genes to the whole plant. - Funct. Plant Biol. 30: 239-264, 2003. Go to original source...
    10. Chaves, M.M., Pereira, J.S., Maroco, J., Rodrigues, M.L., Ricardo, C.P.P., Osório, M.L., Carvalho, I., Faria, T., Pinheiro, C.: How plants cope with water stress in the field? Photosynthesis and growth. - Ann. Bot. 89: 907-916, 2002. Go to original source...
    11. Crafts-Brandner, S.J., Salvucci, M.E.: Rubisco activase constrains the photosynthetic potential of lraves at high temperature and CO2. - Proc. nat. Acad. Sci USA 97: 13430-13435, 2000. Go to original source...
    12. Damerval, C., De Vienne, D., Zivy, M., Thiellement, H.: Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheatseedling proteins. - Electrophoresis 7: 52-54, 1986. Go to original source...
    13. Demirevska, K., Zasheva, D., Dimitrov, R., Simova-Stoilova, L., Stamenova, M., Feller, U.: Drought stress effects on Rubisco in wheat: changes in the Rubisco large subunit. - Acta Physiol. Plant. 31: 1129-1138, 2009. Go to original source...
    14. Díaz, P., Borsani, A., Monza, J.: Acumulación de prolina en plantas en respuesta al estrés osmotico. - Agrociencia 3: 1-10, 1999.
    15. Efeoğlu, B., Ekmekçi, Y., Çiçek, N.: Physiological responses of three maize cultivars to drought stress and recovery. - South Afr. J. Bot. 75: 34-42, 2009.
    16. Farooq, M., Kobayashi, N., Ito, O., Wahid, A., Serraj, R.: Broader leaves result in better performance of indica rice under drought stress. - J. Plant Physiol. 167: 1066-1075, 2010. Go to original source...
    17. Fischer, R.A., Rees, D., Sayre, K.D., Lu, Z.M., Condon, A.G., Larqué-Saavedra, A.: Wheat yield progress is associated with higher stomatal conductance and photosynthetic rate, and cooler canopies. - Crop Sci. 38: 1467-1475, 1998. Go to original source...
    18. Flexas, J., Diaz-Espejo, A., Galmés J., Kaldenhoff, R., Medrano, H., Ribas-Carbo, M.: Rapid variations of mesophyll conductance in response to changes in CO2 concentration around leaves. - Plant Cell Environ. 30: 1284-1298, 2007. Go to original source...
    19. Flexas, J., Ribas-Carbó, J.M., Bota, J., Galmés, J., Henkle, M., Martínez-Cañellas, S., Medrano, H.: Decreased Rubisco activity during water stress is not induced by decreased relative water content but related to conditions of low stomatal conductance and chloroplast CO2 concentration. - New Phytol. 172: 73-82, 2006. Go to original source...
    20. Foyer, C.H., Parry, M.A.J.: Green shoots of sustainability: plant responses to stress. - Institute of Arable Crops Research Rep. Pp. 16-19, 2000-2001.
    21. Grigorova, B., Vaseva, I., Demirevska, K., Feller, U.: Combined drought and heat stress in wheat: changes in some heat shock proteins. - Biol. Plant. 55: 105-111, 2011. Go to original source...
    22. Hajheidari, M., Abdollahian-Noghabi, M., Askari, H., Heidari, M., Sadeghian, S.Y., Ober, E.S., Salekdeh, G.H.: Proteome analysis of sugar beet leaves under drought stress. - Proteomics 5: 950-960, 2005. Go to original source...
    23. Hare, P.D., Cress, W.A., Van Standen, J.: Proline synthesis and degradation, the model goes elucidating stress related signal transduction. - J. exp. Bot. 50: 413-434, 1999. Go to original source...
    24. Hasegawa, Y., Muraki, T., Tokuyama, T., Iwaki, H., Tatsuno, M., Lau, P.C.: A novel degradative pathway of 2-nitrobenzoate via 3-hydroxyanthranilate in Pseudomonas fluorescens strain KU-7. - FEMS Microbiol. Lett. 190: 185-190, 2000. Go to original source...
    25. Hjernø, K., Roepstorff, P.: Improvement of sequence coverage in peptide mass fingerprinting. - Encyclopedia Genet. Genom Proteom Bioinform. DOI:10.1002/047001153X.g301413, 2005. Go to original source...
    26. Horton, P., Park, K.J., Obayashi, T., Fujita, N., Harada, H., Adams-Collier, C.J., Nakai, K.: WoLF PSORT, protein localization predictor. - Nucl. Acids Res. 35: 585-587, 2007. Go to original source...
    27. Huang, H., Barker, W.C., Chen, Y., Wu, C.H.: ProClass: an integrated database of protein family, function and structure information. - Nucl. Acids Res. 31: 390-392, 2003. Go to original source...
    28. Huo, C.M., Zhao, B.C., Ge, R.C., Shen, Y.Z., Huang, Z.J.: [Proteomic analysis of the salt tolerance mutant of wheat under salt stress.] - Yi Chuan Xue Bao 31: 1408-1414, 2004. [In Chinese]
    29. Hurt, E., Hauska, G.: A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts. - Eur. J. Biochem. 117: 591-595, 1981. Go to original source...
    30. Ishida, H., Nishimori, Y., Sugisawa, M., Makino, A., Mae, T.: The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into 37-kda and 16-kda polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat. - Plant Cell Physiol. 38: 471-479, 1997. Go to original source...
    31. Jiang, C.Z., Rodermel, S.R., Shibles, R.M.: Photosynthesis, Rubisco activity and amount, and their regulation by transcription in senescing soybean leaves. - Plant Physiol. 101: 105-112, 1993. Go to original source...
    32. Johari-Pireivatlou, M., Qasimov, N., Maralian, H.: Effect of soil water stress on yield and proline content of four wheat lines. - Afr. J. Biotechnol. 9: 36-40, 2010.
    33. Kamal, A.H.M., Cho, K., Kim, D.K., Uozumi, N., Chung, K.Y., Lee, S.Y., Choi, J.S., Cho, S.W., Shin, C.S., Woo, S.H.: Changes in physiology and protein abundance in saltstressed wheat chloroplasts. - Mol. Biol. Rep. DOI: 10.1007/s11033-012-1777-7, 2012b Go to original source...
    34. Kamal, A.H.M., Cho, K., Komatsu, S., Uozumi, N., Choi, J.S., Woo, S.H.: Towards an understanding of wheat chloroplasts: a methodical investigation of thylakoid proteome. - Mol. Biol. Rep. 39: 5069-5083, 2012a Go to original source...
    35. Kim, A.R., Kamal, A.H.M., Seo, Y.W., Park, C.S., Nam, J., Kim, S.I., Choi, J.S., Woo, S.H.: Leaf proteome analysis of wheat-rye translocation lines. - Aust. J. Crop Sci. 5: 1670-1677, 2011.
    36. Kim, K.H., Kamal, A.H.M., Shin, K.H., Choi, J.S., Heo, H.Y., Woo, S.H.: Large-scale proteome investigation in wild relatives (A, B and D genomes) of wheat. - Acta biochem. biophys. sin. 42: 709-716, 2010. Go to original source...
    37. Lawlor, D.W., Cornic, G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. - Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    38. Murota, K.I., Ohshita, Y., Watanabe, A., Aso, S., Sato, F., Yamada, Y.: Changes related to salt tolerance in thylakoid membranes of photoautotrophically cultured green tobacco cells. - Plant Cell Physiol. 35: 107-113, 1994.
    39. Nakai, K., Kanehisa, M.: Expert system for predicting protein localization sites in gram-negative bacteria. - Proteins 11: 95-110, 1991. Go to original source...
    40. Nilsen, E.T., Orcutt, D.M.: The Physiology of Plants under Stress. - John Wiley & Sons, New York 1996.
    41. O'Farrell, P.H.: High resolution two-dimensional electrophoresis of proteins. - J. biol. Chem. 250: 4007-4021, 1975. Go to original source...
    42. Ort, D.R.: When there is too much light. - Plant Physiol. 125: 29-32, 2001. Go to original source...
    43. Paakkonen, E., Vahala, J., Pohjolai, M., Holopainen, T., Karenlampi, L.: Physiological, stomatal and ultrastructural ozone responses in birch (Betula pendula Roth.) are modified by water stress. - Plant Cell Environ. 21: 671-684, 1998. Go to original source...
    44. Peng, Z., Wang, M., Li, F., Lv, H., Li, C., Xia, G.: A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat. - Mol. cell. Proteomics 8: 2676-2686, 2009. Go to original source...
    45. Porra, R.J.: The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. - Photosyn. Res. 73: 149-156, 2002. Go to original source...
    46. Pradet, A., Raymond, P.: Adenine nucleotide ratios and adenylate energy charge in energy metabolism. - Annu. Rev. Plant Physiol. 34: 199-224, 1983. Go to original source...
    47. Quarrie, S.A., Jones, H.G.: Genotypic variation in leaf water potential, stomatal conductance and abscisic acid concentration in spring wheat subjected to artificial drought stress. - Ann. Bot. 44: 323-332, 1979. Go to original source...
    48. Ribas-Carbo, M., Taylor, N.L., Giles, L., Busquets, S., Finnegan, P.M., Day, D.A., Lambers, H., Medrano, H., Berry, J.A., Flexas, J.: Effects of water stress on respiration in soybean leaves. - Plant Physiol. 139: 466-473, 2005. Go to original source...
    49. Richards, R.A., Condon, A.G., Rebetzke, G.J.: Traits to improve yield in dry environments. - In: Reynolds, M.P., Ortiz Monasterio, J.I., and McNab. A. (ed.): Application of Physiology in Wheat Breeding. Pp. 88-100, CIMMYT, Mexico City 2001.
    50. Rodrigues, F.A., Da Graça, J.P., De Laia, M.L., Nhani, A., Jr., Galbiati, J.A., Ferro, M.I.T., Ferro, J.A., Zingaretti, S.M.: Sugarcane genes differentially expressed during water deficit. - Biol. Plant. 55: 43-53, 2011. Go to original source...
    51. Roemer, S., Hugueney, P., Bouvier, F., Camara, B., Kuntz, M.: Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum. - Biochem. biophys. Res. Commun. 196: 1414-1421, 1993. Go to original source...
    52. Rosales-Serna, R., Kohashi-Shibata, J., Acosta-Gallego, J.A., López, C.T., Cereceres, J.O., Kelly, J.D.: Biomass distribution, maturity acceleration and yield in droughtstressed common bean cultivars. - Field Crops Res. 85: 203-211, 2004. Go to original source...
    53. Salekdeh, G.H., Komatsu, S.: Crop proteomics: aim at sustainable agriculture of tomorrow. - Proteomics 7: 2976-2996, 2007. Go to original source...
    54. Salekdeh, G.H., Siopongco, J., Wade, L.J., Ghareyazie, B., Bennett, J.: Proteomic analysis of rice leaves during drought stress and recovery. - Proteomics 2: 1131-1145, 2002. Go to original source...
    55. Schwartz, A., Ilan, N., Schwarz, M., Scheaffer, J., Assmann, S.M., Schroeder J.I.: Anion-channel blockers inhibit S-type anion channels and abscisic acid responses in guard cells. - Plant Physiol. 109: 651-658, 1995. Go to original source...
    56. Serraj, R., Sinclair, T.R.: Osmolyte accumulation, can it really help increase crop yield under drought conditions? - Plant Cell Environ. 25: 333-341, 2002. Go to original source...
    57. Shen, X., Zhou, Y., Duan, L., Li, Z., Eneji, A.E., Li, J.: Silicon effects on photosynthesis and antioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. - J. Plant Physiol. 167: 1248-1252, 2010. Go to original source...
    58. Stefanovic, S., Pfeil, B.E., Palmer, J.D., Doyle, J.J.: Relationships among phaseoloid legumes based on sequences from eight chloroplast regions. - Syst. Bot. 34: 115-128, 2009. Go to original source...
    59. Sugihara, K., Hanagata, N., Dubinsky, Z., Baba, S., Karube, I.: Molecular characterization of cDNA encoding oxygenevolving enhancer protein 1 increased by salt treatment in the mangrove Bruguiera gymnorrhiza. - Plant Cell Physiol. 41: 1279-1285, 2000. Go to original source...
    60. Tezara, W., Mitchell, V.J., Driscoll, S.D., Lawlor, D.W.: Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. - Nature 401: 914-917, 1999. Go to original source...
    61. Thapa, G, Dey, M., Sahoo, L., Panda, S.K.: An insight into the drought stress induced alterations in plants. - Biol. Plant. 55: 603-613, 2011. Go to original source...
    62. Vendruscolo, E.C., Schuster, I., Pileggi, M., Scapim, C.A., Molinari, H.B., Marur, C.J., Vieira, L.G.: Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat. - J. Plant Physiol. 164: 1367-1376, 2007. Go to original source...
    63. Voetberg, G.S., Sharp, R.E.: Growth of the maize primary root in low water potentials. III. Roles of increased proline depositions in osmotic adjustment. - Plant Physiol. 96: 125-130, 1991. Go to original source...
    64. Wardley, T.A., Bhalla, P.L. Dalling, M.J.: Changes in the number and composition of chloroplasts during senescence of mesophyll cells of attached and detached leaves of wheat (Triticum aestivum L.). - Plant Physiol. 75: 421-424, 1984. Go to original source...
    65. Weiss, W., Gorg, A.: Two-dimensional electrophoresis for plant proteomics. - Methods mol. Biol. 355: 121-143, 2007. Go to original source...
    66. Worden, A.Z., Lee, J.H., Mock, T.: Green evolution and dynamic adaptations revealed by genomes of the marine picoeukaryotes Micromonas. - Science 324: 268-272, 2009. Go to original source...
    67. Zorb, C., Herbst, R., Forreiter, C., Schubert, S.: Short-term effects of salt exposure on the maize chloroplast protein pattern. - Proteomics 9: 4209-4220, 2009. Go to original source...

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