biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:410-416, 2013 | DOI: 10.1007/s10535-013-0304-6

Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana

K. Sala1,*, I. Potocka1, E. Kurczynska1
1 Laboratory of Cell Biology, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland

The aim of the present study was to describe the occurrence of three pectic epitopes, recognized by JIM7, LM19, and LM5 antibodies, during somatic (SE) and zygotic (ZE) embryogenesis in Arabidopsis thaliana. The epitopes recognized by JIM7 and LM19 antibodies showed different distributions during SE stages. Moreover, in the early stages of somatic embryo development, a cytoplasmic occurrence of LM19 epitope was detected. Distribution of a pectic epitope recognized by LM5 antibody corresponded to a vascular system differentiation pattern. Occurrence of LM5 epitope was the same in both zygotic and somatic embryos and often restricted to newly synthesized walls of two adjacent cells. These data suggest that both low and high methyl-esterified pectins (recognized by LM19 and JIM7 antibodies, respectively) are developmentally regulated during SE stages and (1→4)-β-D-galactan epitope (recognized by LM5 antibody) may play a role in cell cytokinesis.

Keywords: galactan; immunofluorescence microscopy; JIM7; LM19; and LM5 antibodies; pectin methyl-esterification
Subjects: pectins; pectic epitopes; in vitro culture; somatic embryogenesis; galactan; immunofluorescence microscopy; pectin methyl-esterification; cell wall

Received: June 14, 2011; Accepted: October 30, 2012; Published: September 1, 2013  Show citation

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Sala, K., Potocka, I., & Kurczynska, E. (2013). Spatio-temporal distribution and methyl-esterification of pectic epitopes provide evidence of developmental regulation of pectins during somatic embryogenesis in Arabidopsis thaliana. Biologia plantarum, 57(3), 410-416. doi: 10.1007/s10535-013-0304-6
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