biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 57:718-724, 2013 | DOI: 10.1007/s10535-013-0335-z

Exogenous salicylic acid enhances wheat drought tolerance by influence on the expression of genes related to ascorbate-glutathione cycle

G. Z. Kang1,*, G. Z. Li1, G. Q. Liu1, W. Xu1, X. Q. Peng1, C. Y. Wang1, Y. J. Zhu1, T. C. Guo1,*
1 Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou, P.R. China

Treatment with 0.5 mM salicylic acid (SA) significantly alleviated growth inhibition induced by drought in wheat seedlings, manifested by less decreassed fresh mass, dry mass, plant height, root length, and less increased lipid peroxidation. Under drought stress, SA significantly increased the content of ascorbate (ASA) and glutathione (GSH). We determined the full-length cDNA sequences of genes encoding the glutathione-S-transferase 1 (GST1) and 2 (GST2) and we also measured the transcription of eight genes related to ASA-GSH cycle. The results indicated that exogenous SA significantly enhanced the transcription of GST1, GST2, glutathione reductase (GR), and monodehydroascorbate reductase (MDHAR) genes during almost the entire drought period, but only increased those of dehydroascorbate reductase (DHAR) at 12 h, glutathione peroxidase (GPX1) at 48 h, phospholipid hydroperoxide glutathione peroxidase (GPX2) at 12 and 24 h, and glutathione synthetase (GSHS) at 12, 24, and 48 h. This implies that SA alleviates the detrimental effects of drought stress on wheat seedling growth by influencing the ASA-GSH cycle.

Keywords: dehydroascorbate reductase; glutathione reductase; glutathione synthetase; glutathione-S-transferase; monodehydroascorbate reductase
Subjects: salicylic acid; stress tolerance; gene expression; ascorbate-glutathione cycle; growth; malondialdehyde; ascorbate; glutathione; amino acid sequences; dehydroascorbate reductase; glutathione peroxidase; glutathione reductase; glutathione-S-transferase; monodehydroascorbate reductase; wheat

Received: June 7, 2012; Accepted: March 28, 2013; Published: December 1, 2013  Show citation

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Kang, G.Z., Li, G.Z., Liu, G.Q., Xu, W., Peng, X.Q., Wang, C.Y., Zhu, Y.J., & Guo, T.C. (2013). Exogenous salicylic acid enhances wheat drought tolerance by influence on the expression of genes related to ascorbate-glutathione cycle. Biologia plantarum, 57(4), 718-724. doi: 10.1007/s10535-013-0335-z
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