biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:689-696, 2014 | DOI: 10.1007/s10535-014-0445-2

Dynamic QTL analysis of the Na+ content, K+ content, and Na+/K+ ratio in rice roots during the field growth under salt stress

J. Sun1, D. T. Zou1,*, F. S. Luan2,*, H. W. Zhao1, J. G. Wang1, H. L. Liu1, D. W. Xie1, D. Q. Su3, J. Ma1, Z. L. Liu1
1 College of Agriculture, Northeast Agricultural University, Harbin, P.R. China
2 College of Horticulture, Northeast Agricultural University, Harbin, P.R. China
3 Seed Supervise Bureau of Heilongjiang Province, Harbin, P.R. China

Rice (Oryza sativa L.) is seriously impacted by global soil salinization. To determine the quantitative trait loci (QTLs) related to salt tolerance in rice roots, F2:3 and BC1F2:3 populations derived from a cross between the cv. Dongnong 425 of high quality and yield and the salt-tolerant cv. Changbai 10, were studied at different development stages. Two genetic linkage maps of F2:3 and BC1F2:3 populations were constructed. A 66 mM NaCl solution was used to irrigate the field and to analyze the dynamic QTL of some rice root traits. Using unconditional and conditional QTL mapping methods, 30 unconditional QTLs and 16 conditional QTLs related to the 6 root traits were detected on the 9 rice chromosomes during different developmental stages. Fourteen pairs of unconditional and conditional QTLs were detected at the identical developmental stage in the identical population. A number of QTLs were detected at different developmental stages, however, many did not appear at the last stage. Remarkably, qRKC1 appeared continuously at multiple stages in both the populations suggesting its key role in regulating the salt tolerance of rice roots.

Keywords: developmental stages; genetic linkage map; Oryza sativa
Subjects: QTL; sodium; potassium; salt stress; linkage mapping; developmental stages; rice

Received: December 18, 2013; Revised: March 24, 2014; Accepted: March 25, 2014; Published: December 1, 2014  Show citation

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Sun, J., Zou, D.T., Luan, F.S., Zhao, H.W., Wang, J.G., Liu, H.L., ... Liu, Z.L. (2014). Dynamic QTL analysis of the Na+ content, K+ content, and Na+/K+ ratio in rice roots during the field growth under salt stress. Biologia plantarum, 58(4), 689-696. doi: 10.1007/s10535-014-0445-2
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    References

    1. Ahmadi, N., Negrão, S., Katsantonis, D., Frouin, J., Ploux, J., Letourmy, P., Droc, G., Babo, P., Trindade, H., Bruschi, G., Greco, R., Oliveira, M. M., PiVanelli, P., Courtois, B.: Targeted association analysis identified japonica rice varieties achieving Na+/K+ homeostasis without the allelic make-up of the salt tolerant indica variety Nona Bokra. - Theor. appl. Genet. 123: 881-895, 2011. Go to original source...
    2. Atchley, W.R., Zhu, J.: Developmental quantitative genetics, conditional epigenetical variability and growth in mice. - Genetics 147: 765-776, 1997. Go to original source...
    3. Barakat, M.N., Wahba, L.E., Milad, S.I.: Molecular mapping of QTLs for wheat flag leaf senescence under water-stress. - Biol. Plant. 57: 79-84, 2013. Go to original source...
    4. Bradshaw, H.D., Stettler, R.F.: Molecular genetics of growth and development in Populus: IV. Mapping QTLs with large effects on growth, form, and phenology traits in a forest tree. - Genetisc 139: 963-973, 1995. Go to original source...
    5. Chai, L., Zhang, J., Pan, X.B., Zhang, F., Zheng, T.Q., Zhao, X.Q., Wang, W.S., Ali, J., Xu, J.L., Li, Z.K.: Advanced backcross QTL analysis for the whole plant growth duration salt tolerance in rice (Oryza sativa L.). - J. int. Agr. 13: 1609-1620, 2013. Go to original source...
    6. Chen, X., Temnykh, S., Xu, Y., Cho, Y.G., McCouch, S.R.: Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). - Theor. appl. Genet. 95: 553-567, 1997. Go to original source...
    7. Clarkson, D.T., Hanson, J.B.: The mineral-nutrition of higher plants. - Annu. Rev. Plant. Physiol. Plant mol. Biol. 31: 239-298, 1980. Go to original source...
    8. Gregorio, G.B., Senadhira, D., Mendoza, R.D., Manigbas, R.D., Roxas, N.L., Guerta, C.Q.: Progress in breeding for salinity tolerance and associated abiotic stress in rice. - Field Crops Res. 76: 91-101, 2002. Go to original source...
    9. Han, Y.P., Xie, D.W., Teng, W.L., Zhang, S.Z., Chang, W., Li, W.B.: Dynamic QTL analysis of linolenic acid content in different developmental stages of soybean seed. - Theor. appl. Genet. 122: 1481-1488, 2011. Go to original source...
    10. International Rice Genome Sequencing Project (IRGSP). The map-based sequence of the rice genome. - Nature 436: 793-800, 2005. Go to original source...
    11. Kim, D.M., Ju, H.G., Kwon, T.R., Oh, C.S., Ahn, S.N.: Mapping QTLs for salt tolerance in an introgression line population between japonica cultivars in rice. - J. Crop Sci. Biotechnol. 12: 121-128, 2009. Go to original source...
    12. Kosambi, D.D.: The estimation of map distances from recombination values. - Ann. Eugen. 12: 172-175, 1944. Go to original source...
    13. Lee, S.Y., Ann, J.H., Cha, Y.S., Yun, D.W., Lee, M.C., Ko, J.C., Lee, K.S., Eun, M.Y.: Mapping QTL related to salinity tolerance of rice at the young seedling stage. - Plant Breed. 126: 43-46, 2007. Go to original source...
    14. Li, S.P., Wang, C.S., Chang, X.P., Jing, R.L.: Genetic dissection of developmental behavior of grain weight in wheat under diverse temperature and water regimes. - Genetica 140: 393-405, 2012. Go to original source...
    15. Lin, H.X., Zhu, M.Z., Yano, M., Gao, J.P., Liang, Z.W., Su, W.A., Hu, X.H., Ren, Z.H., Chao, D.Y.: QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance. - Theor. appl. Genet. 108: 253-260, 2004. Go to original source...
    16. Liu, G.F., Zhu, H.T., Liu, S.W., Zeng, R.Z., Zhang, Z.M., Li, W.T., Ding, X.H., Zhao, F.M., Zhang, G.Q.: Unconditional and conditional QTL mapping for the developmental behavior of tiller number in rice (Oryza sativa L.). - Genetica 138: 885-893, 2010. Go to original source...
    17. Matsushita, N., Matoh, T.: Characterization of Na+ exclusion mechanisms of salt-tolerant reed plants in comparison with salt-sensitive rice plants. - Physiol. Plant. 83: 170-176, 1991. Go to original source...
    18. McCouch, S.R., Cho, Y.G., Yano, M., Paul, E., Blinstrub, M., Morishima, H., Kinoshita, T.: Report on QTL nomenclature. - Rice Genet. Newslett. 14: 11-13, 1997.
    19. Moniruzzaman, M., Alam, M.S., Rashid, J.A., Begum, S.N., Islam, M.M.: Marker-assisted backcrossing for identification of salt tolerant rice lines. - Int. J. agr. Res. Innovation Tech. 2: 1-8, 2012. Go to original source...
    20. Munns, R.: Genes and salt tolerance: bringing them together. - New Phytol. 167: 645-663, 2005. Go to original source...
    21. Munns, R., Tester, M.: Mechanisms of salinity tolerance. - Annu. Rev. Plant Biol. 59: 651-681, 2008. Go to original source...
    22. Murray, M.G., Thompson, W.F.: Rapid isolation of high molecular weight plant DNA. - Nucl. Acids Res. 8: 4321-4325, 1980. Go to original source...
    23. Prasad, S.R., Bagali, P.G., Hittalmani, S., Shashidhar, H.E.: Molecular mapping of quantitative trait loci associated with seedling salt tolerance stress in rice (Oryza sativa L). - Curr. Sci. 78: 162-164, 2000.
    24. Qu, Y.Y., Mu, P.P., Zhang, H.L., Chen, C.Y., Gao, Y.M., Tian, Y.X., Wen, F., Li, Z.C.: Mapping QTLs of root morphological traits at different growth stages in rice. - Genetica 133: 187-200, 2008. Go to original source...
    25. Sabouri, H., Rezai, A.M., Moumeni, A., Kavousi, A., Katouzi, M., Sabouri, A.: QTLs mapping of physiological traits related to salt tolerance in young rice seedlings. - Biol. Plant. 53: 657-662, 2009. Go to original source...
    26. Saleh, M.S., Al-Doss, A.A., Elshafei A.A., Moustafa, K.A., Al-Qurainy, F.H., Barakat, M.N.: Identification of new TRAP markers linked to chlorophyll content, leaf senescence, and cell membrane stability in water-stressed wheat. - Biol. Plant. 58: 64-70, 2014. Go to original source...
    27. Shankhdhar, D., Shankhdhar, S.C., Mani, S.C., Pant, R.C.: In vitro selection for salt tolerance in rice. - Biol. Plant. 43: 477-480, 2000. Go to original source...
    28. Thomson, M.J., Ocampo, M., Egdane, J., Rahman, M.A., Sajise, A.G., Adorada, D.L., Tumimbang-Raiz, E., Blumwald, E., Seraj, Z.I., Singh, R.K., Gregorio, G.B., Ismail, A.M.: Characterizing the Saltol quantitative trait locus for salinity tolerance in rice. - Rice 3: 148-160, 2010. Go to original source...
    29. Wang, J., Li, H., Zhang, L., Li, C., Meng, L.: Users' Manual of QTL IciMapping V3.0. Institute of Crop Science, Beijing 2010.
    30. Wang, Z.F., Wang, J.F., Bao, Y.G., Wu, Y.Y., Zhang, H.S.: Quantitative trait loci controlling rice seed germination under salt stress. - Euphytica 178: 297-307, 2011. Go to original source...
    31. Wang, Z.F., Cheng, J.P., Chen, Z.W., Huang, J., Bao, Y.M., Wang, J.F., Zhang, H.S.: Identification of QTLs with main, epistatic and QTL × environment interaction effects for salt tolerance in rice seedlings under different salinity conditions. - Theor. appl. Genet. 125: 807-815, 2012a. Go to original source...
    32. Wang, Z.F., Chen, Z.W., Cheng, J.P., Lai, Y.Y., Wang, J.F., Bao, Y.M., Huang, J., Zhang, H.S.: QTL analysis of Na+ and K+ concentrations in roots and shoots under different levels of NaCl stress in Rice (Oryza sativa L.). - PLoS ONE. 7: e51202, 2012b. Go to original source...
    33. Wu, R., Garg, A.: Engineering rice plants with trehaloseproducing genes improves tolerance to drought, salt and low temperature. - ISBN News Report, http://www.isb.vt.edu, 2003.
    34. Yan, J.Q., Zhu, J., He, C., Benmoussa, M., Wu, P.: Molecular dissection of developmental behavior of plant height in rice (Oryza sativa L.). - Genetics 150: 1257-1265, 1998. Go to original source...
    35. Yao, M.Z., Vang, J.F., Chen, H.Y., Zhai, H.Q., Zhang, H.S.: Inheritance and QTL mapping of salt tolerance in rice. - Rice Sci. 12: 25-32, 2005.
    36. Zaidem, M.L., Mendoza, R.D., Trmimbang, E.B.: Genetic variability of salinity tolerance at different growth stages of rice. - PBGB 2003 Ann. Rep. Las Banos. Philippines: IRRI, 19-20, 2004.
    37. Zhang, Z.H., Liu, Z.H., Cui, Z.T., Hu, Y.M., Wang, B., Tang, J.H.: Genetic analysis of grain filling rate using conditional QTL mapping in maize. - PloS ONE. 8: e56344, 2013. Go to original source...
    38. Zheng, L.N., Zhang, W.W., Chen, X.G., Ma, J., Chen, W.W., Zhao, Z.G., Zhai, H.Q., Wan, J.M.: Dynamic QTL analysis of rice protein content and protein index using recombinant inbred lines. - J. Plant Biol. 54: 321-328, 2011. Go to original source...
    39. Zhu, J.: Analysis of conditional genetic effects and variance components in developmental genetics. - Genetics 141: 1633-1639, 1995. Go to original source...

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