biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 59:74-82, 2015 | DOI: 10.1007/s10535-014-0467-9

A novel DREB transcription factor from Halimodendron halodendron leads to enhance drought and salt tolerance in Arabidopsis

J. -T. Ma1,2, C. -C. Yin2, Q. -Q. Guo3,2, M. -L. Zhou2, Z. -L. Wang1, Y. -M. Wu2,*
1 Grassland Research Institute, Chinese Academy of Agricultural Sciences, Huhhot, Nei Mongol, P.R. China
2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
3 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand

A new member of the APETALA2/ethylene responsive element binding protein (AP2/EREBP) transcription factor family, HhDREB2, was isolated from Halimodendron halodendron. Based on the similarity of the AP2/ERF domain, HhDREB2 was classified into A-5 group of the DREB subfamily. The expression of HhDREB2 gene was induced by drought, high salinity, and low temperature, but not by exogenous plant hormones. Trans-activity assay demonstrated that HhDREB2 gene encodes a transcription activator. Furthermore, over-expression of HhDREB2 gene under the stress-inducible rd29A promotor in Arabidopsis resulted in enhanced tolerance to salt and drought stresses. The overall results reveal that HhDREB2 functioned as important transcription factor in regulation of stress-responsive signaling in plants and may be used for improving plant tolerance to abiotic stresses.

Keywords: abiotic stress; abscisic acid; auxin; cytokinin; gibberelic acid; expression pattern; transcriptional regulation; transgenic plant
Subjects: DREB transcription factor; drought tolerance; salt tolerance; abscisic acid; auxin; cytokinin; gibberellic acid; nucleotide sequence; phylogenetic tree; polyethylene glycol; transgenic plants; malondialdehyde; soluble sugars

Received: October 29, 2013; Revised: May 19, 2014; Accepted: May 20, 2014; Published: January 1, 2015  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Ma, J.-T., Yin, C.-C., Guo, Q.-Q., Zhou, M.-L., Wang, Z.-L., & Wu, Y.-M. (2015). A novel DREB transcription factor from Halimodendron halodendron leads to enhance drought and salt tolerance in Arabidopsis. Biologia plantarum59(1), 74-82. doi: 10.1007/s10535-014-0467-9
Download citation

References

  1. Agarwal, P., Agarwal, P.K., Nair, S., Sopory, S.K., Reddy, M.K.: Stress-inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity. - Mol. Genet. Genomics 277: 189-198, 2007. Go to original source...
  2. Behnam, B., Kikuchi, A., Celebi-Toprak, F., Kasuga, M., Yamaguchi-Shinozaki, K., Watanabe, K.N.: Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato. - Plant Cell Rep. 26: 1275-1282, 2007. Go to original source...
  3. Bouaziz, D., Pirrello, J., Charfeddine, M., Hammami, A., Jbir, R., Dhieb, A., Bouzayen, M., Gargouri-Bouzid, R.: Overexpression of StDREB1 transcription factor increases tolerance to salt in transgenic potato plants. - Mol. Biotechnol. 54: 803-817, 2012. Go to original source...
  4. Chen, M., Wang, Q.Y., Cheng, X.G., Xu, Z.S., Li, L.C., Ye, X.G., Xia, L.Q., Ma, Y.Z.: GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants. - Biochem. biophys. Res. Commun. 353: 299-305, 2007. Go to original source...
  5. Chen, M., Xu, Z.S., Xia, L.Q., Li, L.C., Cheng, X.G., Dong, J.H., Wang, Q.Y., Ma, Y.Z.: Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.). - J. exp. Bot. 60: 121-135, 2009. Go to original source...
  6. Clough, S.J., Bent, A.F.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. - Plant J. 16: 735-743, 1998. Go to original source...
  7. Cui, M., Zhang, W., Zhang, Q., Xu, Z., Zhu, Z., Duan, F., Wu, R.: Induced over-expression of the transcription factor OsDREB2A improves drought tolerance in rice. - Plant Physiol. Biochem. 49: 1384-1391, 2011. Go to original source...
  8. Cui, Y., Wang, Q.: Physiological responses of maize to elemental sulphur and cadmium stress. - Plant Soil Environ. 52: 523-529, 2006. Go to original source...
  9. Dubouzet, J.G., Sakuma, Y., Ito, Y., Kasuga, M., Dubouzet, E.G., Miura, S., Seki, M., Shinozaki, K., Yamaguchi-Shinozaki, K.: OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. - Plant J. 33: 751-763, 2003. Go to original source...
  10. Gutha, L.R., Reddy, A.R.: Rice DREB1B promoter shows distinct stress-specific responses, and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance. - Plant mol. Biol. 68: 533-555, 2008. Go to original source...
  11. Huang, B., Jin, L., Liu, J.: Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton. - Sci. China C. Life Sci. 50: 7-14, 2007. Go to original source...
  12. Huang, B., Liu, J.Y.: Cloning and functional analysis of the novel gene GhDBP3 encoding a DRE-binding transcription factor from Gossypium hirsutum. - Biochim. biophys. Acta 1759: 263-269, 2006. Go to original source...
  13. Kinkema, M., Fan, W., Dong, X.: Nuclear localization of NPR1 is required for activation of PR gene expression. - Plant Cell 12: 2339-2350, 2000. Go to original source...
  14. Lei, Y., Korpelainen, H., Li, C.: Physiological and biochemical responses to high Mn concentrations in two contrasting Populus cathayana populations. - Chemosphere 68: 686-694, 2007. Go to original source...
  15. Li, K.R., Wang H.H., Han G., Wang Q.J., Fan J.: Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. - New Forests 35: 255-266, 2008. Go to original source...
  16. Li, M.R., Li, Y., Li, H.Q., Wu, G.J.: Improvement of paper mulberry tolerance to abiotic stresses by ectopic expression of tall fescue FaDREB1. - Tree Physiol. 32: 104-113, 2012. Go to original source...
  17. Liu, N., Zhong, N.Q., Wang, G.L., Li, L.J., Liu, X.L., He, Y.K., Xia, G.X.: Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens. - Planta 226: 827-838, 2007. Go to original source...
  18. Liu, Q., Kasuga, M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K., Shinozaki, K.: Two transcription factors, DREB1 and DREB2, with an EREBPAP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-sponsive gene expression, respectively, in Arabidopsis. - Plant Cell 10: 1391-1406, 1998. Go to original source...
  19. Qin, F., Kakimoto, M., Sakuma, Y., Maruyama, K., Osakabe, Y., Tran, L.S., Shinozaki, K., Yamaguchi-Shinozaki, K.: Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L. - Plant J. 50: 54-69, 2007. Go to original source...
  20. Roychoudhury, A., Paul, S., Basu, S.: Cross-talk between abscisic acid-dependent and abscisic acid-independent pathways during abiotic stress. - Plant Cell Rep. 32: 985-1006, 2013. Go to original source...
  21. Sakuma, Y., Maruyama, K., Osakabe, Y., Qin, F., Seki, M., Shinozaki, K., Yamaguchi-Shinozaki, K.: Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. - Plant Cell 18: 1292-1309, 2006. Go to original source...
  22. Santner, A., Estelle, M.: Recent advances and emerging trends in plant hormone signalling. - Nature 459: 1071-1078, 2009. Go to original source...
  23. Sazegari, S., Niazi, A.: Isolation and molecular characterization of wheat (Triticum aestivum) dehydration responsive element binding factor (DREB) isoforms. - Aust. J. Crop Sci. 6: 1037-1044, 2013.
  24. Tang, M., Liu, X.F., Deng, H.P., Shen, S.H.: Over-expression of JcDREB, a putative AP2/EREBP domain-containing transcription factor gene in woody biodiesel plant Jatropha curcas, enhances salt and freezing tolerance in transgenic Arabidopsis thaliana. - Plant Sci. 181: 623-631, 2011. Go to original source...
  25. Xu, Z.S., Ni, Z.Y., Li, Z.Y., Li, L.C., Chen, M., Gao, D.Y., Yu, X.D., Liu, P., Ma, Y.Z.: Isolation and functional characterization of HvDREB1 - a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare. - J. Plant Res. 122: 121-130, 2009. Go to original source...
  26. Xu, Z.S., Ni, Z.Y., Liu, L., Nie, L.N., Li, L.C., Chen, M., Ma, Y.Z.: Characterization of the TaAIDFa gene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat. - Mol. Genet. Genomics 280: 497-508, 2008. Go to original source...
  27. Yamaguchi-Shinozaki, K., Shinozaki, K.: Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. - Plant Biol. 57: 781-803, 2006. Go to original source...
  28. Yang, W., Liu, X.D., Chi, X.J., Wu, C.A., Li, Y.Z., Song, L.L., Liu, X.M., Wang, Y.F., Wang, F.W., Zhang, C., Liu, Y., Zong, J.M., Li, H.Y.: Dwarf apple MbDREB1 enhances plant tolerance to low temperature, drought, and salt stress via both ABA-dependent and ABA-independent pathways. - Planta 233: 219-229, 2011. Go to original source...
  29. Zhao, J., Ren, W., Zhi, D., Wang, L., Xia, G.: Arabidopsis DREB1A/CBF3 bestowed transgenic tall fescue increased tolerance to drought stress. - Plant Cell Rep. 26: 1521-1528, 2007. Go to original source...
  30. Zhou, M.L., Ma, J.T., Pang, J.F., Zhang, Z.L., Tang, Y.X., Wu, Y.M.: Regulation of plant stress response by dehydration responsive element binding (DREB) transcription factors. - Afr. J. Biotechnol. 9: 9255-9279, 2010.
  31. Zhou, M.L., Ma, J.T., Zhao, Y.M., Wei, Y.H., Tang, Y.X., Wu, Y.M.: Improvement of drought and salt tolerance in Arabidopsis and Lotus corniculatus by overexpression of a novel DREB transcription factor from Populus euphratica. - Gene 506: 10-17, 2012. Go to original source...