biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 59:147-153, 2015 | DOI: 10.1007/s10535-014-0469-7

Exogenous sucrose influences antioxidant enzyme activities and reduces lipid peroxidation in water-stressed cucumber leaves

Y. -Y. Cao1,2, M. -T. Yang2, S. -Y. Chen2, Z. -Q. Zhou2, X. Li2, X. -J. Wang2, J. -G. Bai2,*
1 School of Agriculture and Biology, Shanghai Jiao Tong University/State Key Laboratory of Microbial Metabolism, Shanghai, P. R. China
2 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, P.R. China

To investigate whether exogenous sucrose can protect cucumber from water stress, cucumber (Cucumis sativus L.) seedlings were pretreated with 90 mM sucrose or 90 mM mannitol for 1 d and then were dehydrated with 10 % (m/v) polyethylene glycol (PEG) 6000 for further 1 d. Dehydration inhibited plant growth and decreased osmotic potential and relative water content (RWC) in leaves. The pretreatment with 90 mM sucrose further reduced the osmotic potential but increased the RWC and alleviated the growth inhibition. Compared with the PEG treatment alone, the combination of sucrose + PEG increased the activities of superoxide dismutase, guaiacol peroxidase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, ascorbate peroxidase, and glutathione peroxidase, and elevated the content of endogenous sucrose, glucose, and fructose together with the activities of soluble acid invertase and neutral invertase. This was in accordance with the enhanced transcription of genes encoding copper/zinc superoxide dismutase, guaiacol peroxidase, and glutathione reductase. Furthermore, the sucrose pretreatment decreased the content of malondialdehyde and hydrogen peroxide and increased the content of ascorbate, reduced glutathione, and proline under the dehydration. Taken together, the pretreatment with 90 mM sucrose, but much less with mannitol, induced antioxidants, proline, and soluble sugars and thus reduced dehydration-caused damage to the cucumber seedlings.

Keywords: ascorbate-glutathione cycle; Cucumis sativus; fructose; gene expression; glucose; H2O2; invertase; malondialdehyde; polyethylene glycol; proline
Subjects: sucrose; water stress; ascorbate-glutathione cycle; fructose; glucose; invertase; malondialdehyde; proline; hydrogen peroxide; superoxide anion; superoxide dismutase; peroxidase; osmotic potential; relative water content; ascorbate peroxidase; glutathione reductase; cucumber

Received: October 15, 2013; Revised: July 1, 2014; Accepted: August 18, 2014; Published: January 1, 2015  Show citation

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Cao, Y.-Y., Yang, M.-T., Chen, S.-Y., Zhou, Z.-Q., Li, X., Wang, X.-J., & Bai, J.-G. (2015). Exogenous sucrose influences antioxidant enzyme activities and reduces lipid peroxidation in water-stressed cucumber leaves. Biologia plantarum, 59(1), 147-153. doi: 10.1007/s10535-014-0469-7
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