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  • Ecological and Environmental Microbiology
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Antagonistic interactions between fungal rice pathogenFusarium Verticillioides (Sacc.) Nirenberg andTrichoderma harzianum Rifai

Annals of Microbiology volume 59, pages 259–266 (2009)Cite this article

Abstract

Trichoderma harzianum has been found to be a competitor and mycoparasite ofFusarium verticillioides which causes foot rot disease on rice. The experiment was undertaken macroscopically and microscopically. In total 6 treatments were performed combining three water activities (0.95, 0.98, 0.995) and two temperatures (15 and 25 °C). At all conditions tested, except at 0.95a w and 15 °C.Trichoderma harzianum acted as biocontrol agent trough two synergistic mechanisms — competition and mycoparasitism — overF. verticillioides. At 0.95a w and 15 °C mutual antagonism by contact was observed. The effect of abiotic factors water activity and temperature on fungal growth was determined.

References

  • Bacon C.H.W., Yates I.E., Hinton D.M., Meredith F. (2001). Biological control ofFusarium moniliforme in maize. Environ. Health Perspect., 109: 325–332.

    Article  PubMed  CAS  Google Scholar 

  • Bevivino A., Sarrocco S., Dalmastri C., Tabacchioni S., Cantale C., Chiarini L. (1998). Characterization of a free-living maizerhizosphere population ofBurkholderia cepacia: Effect of seed treatment on disease suppression and growth promotion of maize. FEMS Microbiol. Ecol., 27: 225–237.

    Article  CAS  Google Scholar 

  • Bevivino A., Dalmastri C., Tabacchioni S., Chiarini L. (2000). Efficacy ofBurkholderia cepacia MCI 7 in disease suppression and growth promotion of maize. Biol. Fert. Soils, 31: 225–231.

    Article  Google Scholar 

  • Calistru C., McLean M., Berjak P. (1997a).In vitro studies on the potential for biological control ofAspergillus flavus andFusarium moniliforme byTrichoderma species. 1. Macroscopical and microscopical observations of fungal interactions. Mycopathologia, 139: 115–121.

    Article  PubMed  CAS  Google Scholar 

  • Calistru C., McLean M., Berjak P. (1997b).In vitro studies on the potential for biological control ofAspergillus flavus andFusarium moniliforme byTrichoderma species. A study of the production of extracellular metabolites byTrichoderma species. Mycopathologia, 137: 115–124.

    Article  PubMed  CAS  Google Scholar 

  • Cavaglieri L.R., Passone A., Etcheverry M.G. (2004) Correlation between screening procedures to select root endophytes for biological control ofFusarium verticillioides inZea mays L. Biol. Control, 31: 259–267.

    Article  Google Scholar 

  • Cavaglieri L., Orlando J., Rodríguez M.I., Chulze S., Etcheverry M. (2005a). Biocontrol ofBacillus subtilis againstFusarium verticillioides in vitro and at the maize root level. Res. Microbiol., 156: 748–754.

    Article  PubMed  CAS  Google Scholar 

  • Cavaglieri L., Orlando J., Etcheverry M. (2005b).In vitro influence of bacterial mixtures onFusarium verticillioides growth and fumonisin B1 production: effect of seeds treatment on maize root colonization. Lett. Appl. Microbiol., 41: 390–396.

    Article  PubMed  CAS  Google Scholar 

  • Cavaglieri L., Andrés L., Ibáñez M., Etcheverry M.G. (2005c). Rhizobacteria and their potential to controlFusarium verticillioides: effect of maize bacterisation and inoculum density. Antonie van Leeuwenhoek, 87: 179–187.

    Article  PubMed  CAS  Google Scholar 

  • Cheetham J.L., Bazin M.J., Lynch J.M. (1997). Interactions betweenFusarium culmorum and its potential biocontrol agent,Trichoderma harzianum, in a packed-bed, continuous-flow column reactor. Enzyme Microbial Technol., 21: 321–326.

    Article  CAS  Google Scholar 

  • Cohen-Kupiec R., Broglie K.E., Friesem D., Broglie R.M., Chet I. (1999). Molecular characterization of a novel β-1,3-exoglucanase related to mycoparasitism ofTrichoderma harzianum. Gene, 226: 147–154.

    Article  PubMed  CAS  Google Scholar 

  • Cotxarrera L., Trillas-Gay M.I., Steinberg C., Alabouvette C. (2002). Use of sewage sludge compost andTrichoderma Asperellum isolates to suppressFusarium wilt of tomato. Soil Biol. Biochem., 34: 467–476.

    Article  CAS  Google Scholar 

  • Dubey S.C., Suresh M., Singh B. (2007). Evaluation ofTrichoderma species againstFusarium oxysporum f. sp. ciceris for integrated management of chickpea wilt. Biol. Control, 40: 118–127.

    Article  Google Scholar 

  • Griffin D.M. (1963). Soil moisture and the ecology of soil fungi. Biol. Rev. Cambridge, 38: 141–166.

    Article  CAS  Google Scholar 

  • Hinojo M.J., Medina A., Valle-Algarra F.M., Gimeno-Adelantado J.V., Jiménez M., Mateo R. (2006). Fumonisin production in rice cultures ofFusarium verticillioides under different incubation conditions using an optimized analytical method. Food Microbiol., 23: 119–127.

    Article  PubMed  CAS  Google Scholar 

  • Hinton D.M., Bacon C.W. (1995).Enterobacter cloacae is an endophytic symbiont of corn. Mycopathologia, 129: 117–125.

    Article  PubMed  CAS  Google Scholar 

  • Hope R., Magan N. (2003). Two-dimensional environmental profiles of growth, deoxynivalenol and nivalenol production byFusarium culmorum on a wheat-based substrate. Lett. Appl. Microbiol., 37: 70–74.

    Article  PubMed  CAS  Google Scholar 

  • Llorens A., Mateo R., Hinojo M.J., Valle-Algarra F.M., Jiménez M. (2004). Influence of environmental factors on the biosynthesis of type B trichothecenes by isolates ofFusarium spp. from Spanish crops. Int. J. Food Microbiol., 94: 43–54.

    Article  PubMed  CAS  Google Scholar 

  • Magan N., Lacey J. (1984). The effect of water activity, temperature and substrate on interactions between field and storage fungi. Trans. Br. Mycol. Soc., 82: 83–93.

    Article  Google Scholar 

  • Marín S., Sanchis V., Magan N. (1995). Water activity, temperature and pH effects on growth ofFusarium moniliforme andF. Proliferatum isolates from maize. Can. J. Microbiol., 41: 1063–1070.

    Article  PubMed  Google Scholar 

  • Marín S., Sanchis V., Rull F., Ramos A.J., Magan N. (1998). Colonization of maize grain byFusarium moniliforme andFusarium proliferatum in the presence of competing fungi and their impact on fumonisin production. J. Food Prot., 61: 1489–1496.

    PubMed  Google Scholar 

  • Mew T.W., Gonzales P. (2002). A Handbook of Rice Seedborne Fungi. International Rice Research Institute, and Enfield, N.H (USA), Science Publishers, Inc. Los Baños (Philippines).

    Google Scholar 

  • Miedaner T., Perkowski J. (1996). Correlations amongFusarium Culmorum head blight resistance, fungal colonization and mycotoxin contents in winter rye. Plant Breeding, 115: 347–351.

    Article  CAS  Google Scholar 

  • Paul B. (1999). Suppression ofBotrytis cinerea causing the grey mould disease of grape-vine by an aggressive mycoparasite,Pythium radiosum. FEMS Microbiol. Lett., 176: 25–30.

    Article  CAS  Google Scholar 

  • Picco M., Nesci A., Barros G., Cavaglieri L., Etcheverry M. (1999). Aflatoxin B1 and fumosin B1 in mixed cultures ofAspergillus Flavus andFusarium proliferatum on maize. Nat. Toxins, 7: 331–336.

    Article  PubMed  CAS  Google Scholar 

  • Pitt J.I., Hocking A.D. (1999). Fungi and food spoilage, 2th edn., Aspen Publishers, Inc., New York.

    Google Scholar 

  • Prasad R.D., Rangeshwarana R., Hegde S.V., Anuroop C.P. (2002). Effect of soil and seed application ofTrichoderma harzianum on pigeonpea wilt caused byFusarium udum under field conditions. Crop Prot., 21: 293–297.

    Article  Google Scholar 

  • Reddy O.R., Sathyanarayana N. (2002). Seed-borne fungi of rice and quarantine significance. In: Sreenivasaprasad S., Johnson R., Eds, Major Fungal Diseases of Rice. Dordrecht. Recent Advances, Kluwer Academic Publishers.

  • Rojo F.G., Reynoso M.M., Ferez M., Chulze S.N., Torres A.M. (2007). Biological control byTrichoderma species ofFusarium Solani causing peanut brown root rot under field conditions. Crop Prot., 26: 549–555.

    Article  Google Scholar 

  • Sempere F., Roselló P., Santamarina M.P. (2004). Estudio ecofisiológico deFusarium culmorum (G.W. Smith) Sacc. en condicionesIn vitro. Phytoma, 162: 106–108.

    Google Scholar 

  • Sempere F., Santamarina M.P. (2006a). Ecofisiología deDrechslera Oryzae Subram. & Jain en condicionesIn vitro. Phytoma, 178: 49–50.

    Google Scholar 

  • Sempere F., Santamarina M.P. (2006b). Microscopic and macroscopic study of the interaction betweenAlternaria alternate (Fr.) Keissler andNigrospora oryzae (Berk. & Broome) Petch. Ann. Microbiol., 56: 101–107.

    Article  Google Scholar 

  • Sempere F., Santamarina M.P. (2007).In vitro biocontrol analysis ofAlternaria alternate (Fr.) Keissler under different environmental conditions. Mycopathologia, 163: 183–190.

    Article  PubMed  CAS  Google Scholar 

  • Sempere F., Roselló J., Santamarina M.P. (2007). Interacciones competitivas entreFusarium sambucinum Fuckel yPhoma glomerata (Corda) Wollenweber & Hochapfel en condicionesin vitro. Rev. Iberoam. Micol., 24: 29–33.

    Article  Google Scholar 

  • Shalini, Narayan K.P., Lata, Kotasthane A.S. (2006). Genetic relatedness amongTrichoderma isolates inhibiting a pathogenic fungiRhizoctonia solani. Afr. J. Biotechnol., 5: 580–584.

    CAS  Google Scholar 

  • Ŝrobárová A., Eged Ŝ. (2005).Trichoderma and sulphoethyl glucan reduce maize root rot infestation and fusaric acid content. Plant Soil Environ., 51: 322–327.

    Google Scholar 

  • Thangavelu R., Palaniswami A., Velazhahan R. (2004). Mass production ofTrichoderma harzianum for managing fusarium wilt of banana. Agric. Ecosyst. Environ., 103: 259–263.

    Article  Google Scholar 

  • Velluti A., Marín S., Bettucci L., Ramos A.J., Sanchis V. (2000). The effect of fungal competition on colonization of maize grain byFusarium moniliforme, F. Proliferatum andF. Graminearum and on fumonisin B and zearalenone formation. Int. J. Food Microbiol., 59: 59–66.

    Article  PubMed  CAS  Google Scholar 

  • Wang H., Chang K.F., Hwang S.F., Turnbull G.D., Howard R.J., Blade S.F., Callan N.W. (2005). Fusarium root rot of coneflower seedlings and integrated control usingTrichoderma and fungicides. BioControl, 50: 317–329.

    Article  CAS  Google Scholar 

  • Whipps J.M., McQuilken M.P. (1993). Aspects of biological control of fungal plant pathogens. In: Jones D.J., Ed., Exploitation of Microorganisms. Chapman & Hall. London.

    Google Scholar 

  • Woods D.M., Duniway J.M. (1986). Some effect of water potential on growth, turgor, and respiration ofPhytophtora cryptogea andFusarium moniliforme. Phytopathology, 76: 1248–1253.

    Article  Google Scholar 

  • Yates I.E., Meredith F., Smart W., Bacon C.W., Jaworski A.J. (1999).Trichoderma viride suppresses fumonisin B1 production byFusarium moniliforme. J. Food Prot., 66: 1326–1332.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Ecosistemas Agroforestales, Escuela Técnica Superior del Medio Rural y Enología, Universidad Politécnica de Valencia, Avda. Blasco Ibañez 21, 46010, Valencia, España

    Francisca Sempere & María Pilar Santamarina

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  1. Francisca Sempere
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  2. María Pilar Santamarina
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Correspondence to Francisca Sempere.

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Sempere, F., Santamarina, M.P. Antagonistic interactions between fungal rice pathogenFusarium Verticillioides (Sacc.) Nirenberg andTrichoderma harzianum Rifai. Ann. Microbiol. 59, 259–266 (2009). https://0-doi-org.brum.beds.ac.uk/10.1007/BF03178326

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Annals of Microbiology

ISSN: 1869-2044

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