biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:411-413, 2005 | DOI: 10.1007/s10535-005-0017-6

Establishment of callus and cell suspension cultures of Centella asiatica

S. Nath1, A. K. Buragohain1,*
1 Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Assam, India

Methods for induction of callus and cell suspension cultures have been developed for the medicinally important herb Centella asiatica (L.) Urban. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) analysis showed the presence of asiaticoside in the in vitro grown leaves, callus and cell suspension cultured cells.

Keywords: asiaticoside; auxin; cytokinins; high performance liquid chromatography; thin layer chromatography
Subjects: asiaticoside; auxins; Centella asiatica; cytokinins; in vitro culture, callus induction; in vitro culture, cell culture; medicinal plants; nutrient medium MS (Murashige and Skoog)

Received: June 2, 2003; Accepted: November 2, 2004; Published: September 1, 2005  Show citation

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Nath, S., & Buragohain, A.K. (2005). Establishment of callus and cell suspension cultures of Centella asiatica. Biologia plantarum, 49(3), 411-413. doi: 10.1007/s10535-005-0017-6
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