biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:167-171, 2012 | DOI: 10.1007/s10535-012-0036-z

Cadmium modulates NADPH oxidase activity and expression in sunflower leaves

M. D. Groppa1,*, M. P. Ianuzzo1, E. P. Rosales1, S. C. Vázquez2, M. P. Benavides1
1 Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
2 Cátedra de Microbiología Industrial y Biotecnología, Departamento de Microbiología, Inmunología y Biotecnología Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina

The production of reactive oxygen species (ROS) and the ways by which ROS are generated are very important facts related to heavy metal toxicity in plants. In this work, superoxide anion (O2 .-) generation diminished in cadmium treated sunflower (Helianthus annuus L.) leaf discs, and this reduction was time and Cd-concentration dependent. In line with these findings, we observed that NADPH-dependent oxidase activity was significantly inhibited by 0.1 and 0.5 mM Cd2+ treatments and the expression of the NADPH oxidase putative gene related to O2 .- synthesis in sunflower leaves was 83 % inhibited by 0.1 mM CdCl2 and almost completely depleted by 0.5 mM CdCl2.

Keywords: Helianthus annuus; reactive oxygen species; superoxide anion
Subjects: superoxide anion; cadmium; NADPH oxidase; gene expression; PCR; superoxide dismutase; frost tolerance; ROS; temperature - low

Received: December 28, 2009; Accepted: January 25, 2011; Published: March 1, 2012  Show citation

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Groppa, M.D., Ianuzzo, M.P., Rosales, E.P., Vázquez, S.C., & Benavides, M.P. (2012). Cadmium modulates NADPH oxidase activity and expression in sunflower leaves. Biologia plantarum, 56(1), 167-171. doi: 10.1007/s10535-012-0036-z
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