biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:775-779, 2012 | DOI: 10.1007/s10535-012-0130-2

The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties

H. Hoffmeisterova1,2, T. Moravec1, H. Plchova1, J. Folwarczna1,3, N. Cerovska1,*
1 Laboratory of Virology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic
2 Department of Crop Protection, Czech University of Life Sciences Prague, Prague 6, Czech Republic
3 Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague 2, Czech Republic

The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3'-terminal modification of XCP on the chimera's life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3'-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras.

Keywords: chimeric coat protein; expression of recombinant protein; Nicotiana benthamiana; terminal fusion
Subjects: coat protein; gene construct; gene expression; polyclonal antibodies

Received: July 21, 2011; Accepted: December 7, 2011; Published: December 1, 2012  Show citation

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Hoffmeisterova, H., Moravec, T., Plchova, H., Folwarczna, J., & Cerovska, N. (2012). The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties. Biologia plantarum, 56(4), 775-779. doi: 10.1007/s10535-012-0130-2
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