biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:445-455, 2014 | DOI: 10.1007/s10535-014-0424-7

Isolation and functional characterization of a novel gene coding for flavonoid 3'-hydroxylase from globe artichoke

M. De Palma1, F. Fratianni2, F. Nazzaro2, M. Tucci1,*
1 IBBR Institute of Biosciences and Bioresources, CNR, Portici, Italy
2 Institute of Food Science and Technology, CNR, Avellino, Italy

Globe artichoke (Cynara cardunculus L. var. scolymus) is rich in flavonoids which contribute to its health-promoting properties. With the aim of understanding the genetic control of flavonoid accumulation in artichoke, we isolated an artichoke full-length cDNA sequence encoding flavonoid 3'-hydroxylase (F3'H), a major enzyme of the flavonoid hydroxylation pattern. In silico studies confirmed that the deduced amino acid sequence of CcF3'H is highly similar to F3'Hs isolated from other Asteraceae. The Northern blot analysis demonstrated that CcF3'H was highly expressed in leaves and in specific parts of the heads. Its expression differed slightly among artichoke cultivars. The overexpression of CcF3'H in tobacco plants led to the accumulation of flavonoids and to an increase of flower colour intensity, thus identifying CcF3'H as promising candidate for genetic engineering. CcF3'H represents the first structural gene of the flavonoid biosynthesis isolated from C. cardunculus, and its characterization sheds light on the accumulation of flavonoids.

Keywords: anthocyanins; Cynara cardunculus; expression analysis; flavonoid biosynthesis; transgenic plants
Subjects: flavonoid biosynthesis; anthocyanin biosynthesis; transgenic plans; amino acid sequence; phylogenetic tree; flowering; globe artichoke

Received: April 23, 2013; Revised: February 12, 2014; Accepted: February 18, 2014; Published: September 1, 2014  Show citation

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De Palma, M., Fratianni, F., Nazzaro, F., & Tucci, M. (2014). Isolation and functional characterization of a novel gene coding for flavonoid 3'-hydroxylase from globe artichoke. Biologia plantarum, 58(3), 445-455. doi: 10.1007/s10535-014-0424-7
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