biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 61:95-105, 2017 | DOI: 10.1007/s10535-016-0657-8

Overexpression of UDP-glucose dehydrogenase from Larix gmelinii enhances growth and cold tolerance in transgenic Arabidopsis thaliana

N. N. Li1, L. Chen1, X. H. Li2, Q. Li1, W. B. Zhang2, K. Takechi3, H. Takano3, X. F. Lin1,*
1 College of Life Sciences, Inner Mongolia University, Hohhot, P.R. China
2 College of Forestry, Inner Mongolia Agricultural University, Hohhot, P.R. China
3 Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto, Japan

Uridine diphosphate glucose dehydrogenase (UGDH) plays an important role in biosynthesis of hemicellulose by catalyzing oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in biosynthesis of the plant cell wall. In this study, a UGDH ortholog referred to as LgUGDH was isolated from Larix gmelinii using PCR and rapid amplification of cDNA ends techniques. Real-time PCR shows that the LgUGDH gene was expressed primarily in larch stems in addition to its roots and leaves, and Southern blot analysis indicates that UGDH is encoded by two paralogous genes in L. gmelinii. Overexpression of LgUGDH increased the content of soluble sugars and hemicelluloses and enhanced vegetative growth and cold tolerance in transgenic Arabidopsis thaliana. These results reveal that L. gmelinii UGDH participates in sucrose/polysaccharide metabolism and cell wall biosynthesis and may be a good candidate gene for enhancing plant growth, cold tolerance, and hemicellulose content.

Keywords: hemicellulose; real time PCR; RACE; sugars; UDP-glucuronate
Subjects: gene overexpression; UDP-glucose dehydrogenase; cold tolerance; hemicellulose; Southern blot; transgenic plants; sugars; lignin; cellulose; growth; larch

Received: November 2, 2015; Revised: March 17, 2016; Accepted: March 21, 2016; Published: January 1, 2017  Show citation

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Li, N.N., Chen, L., Li, X.H., Li, Q., Zhang, W.B., Takechi, K., Takano, H., & Lin, X.F. (2017). Overexpression of UDP-glucose dehydrogenase from Larix gmelinii enhances growth and cold tolerance in transgenic Arabidopsis thaliana. Biologia plantarum, 61(1), 95-105. doi: 10.1007/s10535-016-0657-8
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