biologia plantarum

Biologia plantarum 61:305-314, 2017 | DOI: 10.1007/s10535-016-0668-5

Anatomy, photochemical activity, and DNA polymorphism in leaves of dwarf tomato irradiated with X-rays

C. Arena^1,2,*, M. Turano¹, B. Hay Mele³, P. R. Cataletto¹, M. Furia¹, M. Pugliese⁴, V. De Micco³

¹ Department of Biology, University of Naples Federico II, Naples, Italy

² National Research Council of Italy, Institute for Sustainable Plant Protection, Sesto Fiorentino, Italy

³ Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy

⁴ Department of Physics, University of Naples Federico II, Naples, Italy

The response of higher plants to ionising radiation depends on factors related to both radiation properties and plant features including species, cultivar, age, and structural complexity of the target organ. Adult plants of dwarf tomato were irradiated with different doses of X-rays to investigate possible variations in leaf morpho-anatomical traits, photosynthetic efficiency, and genomic DNA. In order to assess if and how responses depend on leaf developmental stage, we analysed two types of leaves; nearly mature leaves (L1) and actively developing leaves (L2), whose lamina size corresponded to 70 and 25 %, respectively, of the lamina size of the fully expanded leaves. The results show that the X-rays prevented full lamina expansion of the L2 leaves at all doses and induced early death of tissue of plants irradiated with doses higher than 20 Gy. Most anatomical modifications were not clearly dose-dependent and the radiation-induced increase in phenolic compounds was irrespective of dose. At high doses of X-rays (50 and 100 Gy), photochemical efficiency decreased significantly in both leaf types, whereas total chlorophyll content significantly decreased only in the L2 leaves. The random amplification of polymorphic DNA data show that the X-rays induced mutagenic effects in the L2 leaves even at low doses despite the absence of severe phenotypic alterations. Genetic structure found in the population of samples corroborates the results of anatomical and eco-physiological analyses: the 20 Gy dose seems to mark the threshold dose above which genetic alterations, structural anomalies, and perturbations in the photosynthetic apparatus become significant, especially in the actively expanding leaves.

Keywords: chlorophyll content; leaf development; photosystem 2; RAPD

Subjects: DNA polymorphism; X-rays; leaf anatomy; cell area; cell shape; epidermis; parenchyma; phenolics; chlorophyll content; chlorophyll a fluorescence; dwarf tomato

Received: January 15, 2016; Revised: July 14, 2016; Accepted: July 18, 2016; Published: June 1, 2017  Show citation