biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 61:494-500, 2017 | DOI: 10.1007/s10535-016-0675-6

Constitutive expression of SlTrxF increases starch content in transgenic Arabidopsis

F. B. Wang1,*, W. L. Kong2, Y. R. Fu3, X. C. Sun1, X. H. Chen1, Q. Zhou1
1 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, Jiangsu, P.R. China
2 Departments of Microbiology and Immunology, University of Rochester Medical Center, Rochester, USA
3 School of Landscape Architecture, Zhejiang A & F University, Lin'an, Zhejiang, China

The plastidic thioredoxin F-type (TrxF) protein plays an important role in plant saccharide metabolism. In this study, a gene encoding the TrxF protein, named SlTrxF, was isolated from tomato. The coding region of SlTrxF was cloned into a binary vector under the control of 35S promoter and then transformed into Arabidopsis thaliana. The transgenic Arabidopsis plants exhibited increased starch accumulation compared to the wild-type (WT). Real-time quantitative PCR analysis showed that constitutive expression of SlTrxF up-regulated the expression of ADP-glucose pyrophosphorylase (AGPase) small subunit (AtAGPase-S1 and AtAGPase-S2), AGPase large subunit (AtAGPase-L1 and AtAGPase-L2) and soluble starch synthase (AtSSS I, AtSSS II, AtSSS III and AtSSS IV) genes involved in starch biosynthesis in the transgenic Arabidopsis plants. Meanwhile, enzymatic analyses showed that the major enzymes (AGPase and SSS) involved in the starch biosynthesis exhibited higher activities in the transgenic plants compared to WT. These results suggest that SlTrxF may improve starch content of Arabidopsis by regulating the expression of the related genes and increasing the activities of the major enzymes involved in starch biosynthesis.

Keywords: ADP-glucose pyrophosphorylase; saccharide biosynthesis; Solanum lycopersicum; soluble starch synthase; tomato
Subjects: thioredoxin; constitutive expression; starch; transgenic plants; ADP-glucose pyrophosphorylase; saccharide biosynthesis; starch synthase; tomato

Received: January 25, 2016; Revised: July 8, 2016; Accepted: July 13, 2016; Published: September 1, 2017  Show citation

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Wang, F.B., Kong, W.L., Fu, Y.R., Sun, X.C., Chen, X.H., & Zhou, Q. (2017). Constitutive expression of SlTrxF increases starch content in transgenic Arabidopsis. Biologia plantarum, 61(3), 494-500. doi: 10.1007/s10535-016-0675-6
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