Photosynthetica 2014, 52(4):548-554 | DOI: 10.1007/s11099-014-0062-5

Fusilade herbicide causes alterations in chloroplast ultrastructure, pigment content and physiological activities of peanut leaves

K. A. Fayez1,2,*, D. E. M. Radwan1, A. K. Mohamed1, A. M. Abdelrahman1
1 Botany Department, Faculty of Science, Sohag University, Sohag, Egypt
2 Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia

Fusilade (fluazifop-p-butyl) is one of the herbicides that inhibit acetyl-CoA carboxylase. The exogenous effect of 30, 60, and 90 ppm fusilade on peanut (Arachis hypogaea L. cv. Giza 5) leaves was studied. With increasing fusilade concentration, the peanut leaf chlorosis appeared after 7-10 d. Declined leaf pigment contents confirmed the leaf chlorosis. Electron microscopic observation of the fusilade-treated (FT) leaves revealed disorganization in the ultrastructure of mesophyll cell chloroplasts. An increase of plastoglobuli occurrence within chloroplasts and degenerated grana thylakoids were observed in FT leaves. Fusilade treatments induced mainly the enhancement of malondialdehyde content and the activities of peroxidases (guaiacol and ascorbate). On contrary, a decrease in H2O2 content, catalase and superoxide dismutase activities was recorded. Enhancements of the guaiacol and ascorbate peroxidase activities were associated with the decreasing H2O2 content in the FT leaves. Hydrogen peroxide seems not to be involved in the oxidative stress of FT leaves. In the FT leaves, the oxidative stress confirmed by chlorophyll degradation and lipid peroxidation might be caused by the other reactive oxygen species probably due to the decrease of superoxide dismutase activity.

Additional key words: antioxidants; Arachis hypogeae; fusilade; lipid peroxidation; ultrastructure

Received: August 1, 2013; Accepted: March 6, 2014; Published: December 1, 2014  Show citation

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Fayez, K.A., Radwan, D.E.M., Mohamed, A.K., & Abdelrahman, A.M. (2014). Fusilade herbicide causes alterations in chloroplast ultrastructure, pigment content and physiological activities of peanut leaves. Photosynthetica, 52(4), 548-554. doi: 10.1007/s11099-014-0062-5
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    References

    1. Alscher, R.G., Erturk, N., Heath, L.S.: Role of superoxide dismutases (SODs) in colltrolling oxidative stress in plants. - J. Exp. Bot. 53: 1331-1341, 2002. Go to original source...
    2. Banaĥ, A., Johansson, I., Stenlid, G. et al.: Free radical scavengers and inhibitors of lipoxygenases as antagonists the herbicides haloxyfop and alloxydim. - Swed. J. Agr. Res. 23: 67-75, 1993.
    3. Banaĥ, A., Banas, W, Stenlid, G et al.: Selective increase in acyl hydrolase activity by graminicides in wheat. - Biochem. Soc. T. 28: 777-779, 2000. Go to original source...
    4. Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. - Anal. Biochem. 44: 276-287, 1971. Go to original source...
    5. Belkebir, A., Benhassaine-Kesri, G.: Sethoxydim treatment inhibits lipid metabolism and enhances the accumulation of anthocyanins in rape (Brassica napus L.) leaves. - Pestic. Biochem. Phys. 107: 120-126, 2013. Go to original source...
    6. Chandlee, J.M., Scandalios, J.G.: Analysis of variants affecting the catalase development program in maize scutellum. - Theor. Appl. Genet. 69: 71-77, 1984. Go to original source...
    7. Chen, S.G., Yin, C.Y., Strasser, R.J. et al.: Reactive oxygen species from chloroplasts contribute to 3-acetyl-5-isopropyltetramic acid-induced leaf necrosis of Arabidopsis thaliana. - Plant Physiol. Bioch. 52: 38-51, 2012. Go to original source...
    8. Christopher, J.T., Holtum, J.A.M.: The dicotyledonous species Erodium moschatum (L) L'Her. ex Aiton is sensitive to haloxyfop herbicide due to herbicide-sensitive acetylcoenzyme A carboxylase. - Planta 207: 275-279, 1998. Go to original source...
    9. Christopher, J.T., Holtum, J.A.M.: Dicotyledons lacking the multisubunit form of the herbicide target enzyme acetyl coenzyme A carboxylase may be restricted to the family Geraniaceae. - Aust. J. Plant Physiol. 27: 845-850. 2000. Go to original source...
    10. Cui, L.E., Yang, H.: Accumulation and residue of napropamide in alfalfa (Medicago sativa) and soil involved in toxic response.- J. Hazard. Mater. 190: 81-86, 2011. Go to original source...
    11. Das, P.K., Bagchi, S.N.: Bentazone and bromoxynil induce H+ and H2O2 accumulation, and inhibit photosynthetic O2 evolution in Synechococcous elongatus PCC7942. - Pestic. Biochem. Phys. 97: 256-261, 2010. Go to original source...
    12. Di Cagno, R., Guidi, L., De Gara, L. et al.: Combined cadmium and ozone treatments affects photosynthesis and ascorbate dependent defences in sunflower. - New Phytol. 151: 627-636, 2001. Go to original source...
    13. Fayez, K.A., Bazaid, S.A.: Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate. - J. Saudi Soc. Agric. Sci. 13: 45-55, 2014. Go to original source...
    14. Fayez, K.A., Hassanein, A.M.: Chlorophyll synthesis retardation and ultrastructural alterations to Solanum tuberosum chloroplasts in Solanum nigrum cells. - Photosynthetica 38: 37-44, 2000. Go to original source...
    15. Fayez, K.A., Kristen, U.: The influence of herbicides on the growth and proline content of primary roots and on the ultrastructure of root caps. - Environ. Exp. Bot. 36: 71-81, 1996. Go to original source...
    16. Fayez, K.A., Mahmoud, S.Y.: Detection and partial characterization of a putative closterovirus affecting Ficus carica: molecular, ultrastructural and physiological aspects of infected leaves. - Acta Physiol. Plant. 33: 2187-2198, 2011. Go to original source...
    17. Fayez, K.A.: Action of photosynthetic diuron herbicide on cell organelles and biochemical constituents of the leaves of two soybean cultivars. - Pestic. Biochem. Phys. 66: 105-115, 2000. Go to original source...
    18. Fecht-Christoffers, M.M., Maier, P., Horst, W.J.: Apoplastic peroxidase and ascorbate are involved in manganese toxicity and tolerance of Vigna unguiculata. - Physiol. Plantarum 117: 237-244, 2003. Go to original source...
    19. Feierabend, J., Winkelhüsener, T.: Nature of photooxidative events in leaves treated with chlorosis-inducing herbicides. - Plant Physiol. 70: 1277-1282, 1982. Go to original source...
    20. Foyer, C.H., Noctor, G.: Oxygen processing in photosynthesis: regulation and signalling. - New Phytol. 146: 359-388, 2000. Go to original source...
    21. Fridovich, I.: Biological effects of the superoxide radical. - Arch. Biochem. Biophys. 247: 1-11, 1986. Go to original source...
    22. Gill, S.S., Tuteja, N.: Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. - Plant Physiol. Bioch. 48: 909-930, 2010. Go to original source...
    23. Gnanamurthy, P., Balasubramaniyan, P.: Weed-management practices and their influence on weed growth and yield of groundnut (Arachis hypogaea). - Indian J. Agron. 43: 122-125, 1998.
    24. Harwood, J.L.: Fatty acid metabolism. - Annu. Rev. Plant Phys. 39: 101-138, 1988. Go to original source...
    25. Harwood, J.L.: Recent advances in the biosynthesis of plant fatty acids. - BBA-Lipid Lipid Met. 1301: 7-56, 1996. Go to original source...
    26. Hernández, J.A., Almansa, M.S.: Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves. - Physiol. Plantarum 115: 251-257, 2002. Go to original source...
    27. Hernández, J.A., Rubio, M., Olmos, E. et al.: Oxidative stress induced by long-term plum pox virus infection in peach (Prunus persica). - Physiol. Plantarum 122: 486-495, 2004. Go to original source...
    28. Horbowicz, M., Sempruch, C., Kosson, R. et al.: Effect of fluazifop-p-butyl treatment on pigments and polyamines level within tissues of non-target maize plants. - Pestic. Biochem. Phys. 107: 78-85, 2013. Go to original source...
    29. Iannelli, A.M., van Breusegem, F., van Montagu, M. et al.: Tolerance to low temperature and paraquat-mediated oxidative stress in two maize genotypes. - J. Exp. Bot. 50: 523-532, 1999. Go to original source...
    30. Jana, S., Choudhuri, M.A.: Glycolate metabolism of three submerged aquatic angiosperms during aging. - Aquat. Bot. 11: 67-77, 1981. Go to original source...
    31. Jiang, L., Yang H.: Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat. - Ecotox. Environ. Saf. 72: 1687-1693, 2009. Go to original source...
    32. Jung, S.Y.: Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. - Plant Sci. 166: 459-466, 2004. Go to original source...
    33. Kawano, T.: Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. - Plant Cell Rep. 21: 829-837, 2003. Go to original source...
    34. Konishi, T., Shinohara, K., Yamada, K. et al.: Acetyl-CoA carboxylase in higher plants: most plants other than gramineae have both the prokaryotic and the eukaryotic forms of this enzyme. - Plant Cell Physiol. 37: 117-122, 1996. Go to original source...
    35. Krieger-Liszkay, A.: Singlet oxygen production in photosynthesis. - J. Exp. Bot. 56: 337-346, 2005. Go to original source...
    36. Lambert, C.E., Bondy. S.C.: Effects of MPTP, MPP+ and paraquat on mitochondrial potential and oxidative stress. - Life Sci. 44: 1277-1284, 1989. Go to original source...
    37. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic biomembranes. - In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Pp. 350-382. Academic Press, San Diego, New York, Berkeley, Boston, London, Sydney, Tokyo, Toronto 1987. Go to original source...
    38. Lukatkin, A.S., Gar'kova., A.N., Bochkarjova, A.S. et al.: Treatment with the herbicide TOPIK induces oxidative stress in cereal leaves. - Pestic. Biochem. Phys. 105: 44-49, 2013. Go to original source...
    39. Luo, X.Y., Sunohara, Y., Matsumoto, H.: Fluazifop-butyl causes membrane peroxidation in the herbicide-susceptible broad leaf weed bristly starbur (Acanthospermum hispidum). - Pestic. Biochem. Phys. 78: 93-102, 2004. Go to original source...
    40. Ma, J.Y., Xu, L.G., Wang, S.F. et al.: Toxicity of 40 herbicides to the green alga Chlorella vulgaris. - Ecotox. Environ. Safe. 51: 128-132, 2002. Go to original source...
    41. Mhamdi, A., Noctor, G., Baker, A.: Plant catalases: Peroxisomal redox guardians. - Arch. Biochem. Biophys. 525: 181-194, 2012. Go to original source...
    42. Mittler, R.: Oxidative stress, antioxidants and stress tolerance. - Trends Plant Sci. 7: 405-410, 2002. Go to original source...
    43. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. - Plant Cell Physiol. 22: 867-880, 1981.
    44. Nikolskaya, T., Zagnitko, O., Tevzadze, G. et al.: Herbicide sensitivity determinant of wheat plastid acetyl-CoA carboxylase is located in a 400-amino acid fragment of the carboxyl transferase domain. - P. Natl. Acad. Sci.-Biol. 96: 14647-14651, 1999. Go to original source...
    45. Ohkawa, H., Ohishi, N., Yagi, K.: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. - Anal. Biochem. 95: 351-358, 1979. Go to original source...
    46. Peixoto, F., Alves-Fernandes D., Santos D. et al.: Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromis niloticus. - Pestic. Biochem. Phys. 85: 91-96, 2006. Go to original source...
    47. Polle, A., Otter, T., Seifert, F.: Apoplastic peroxidases and lignifications in needles of Norway Spruce Picea abies L. - Plant Physiol. 106: 53-60, 1994. Go to original source...
    48. Radwan, D.E.M.: Salicylic acid induced alleviation of oxidative stress caused by clethodim in maize (Zea mays L.) leaves. - Pestic. Biochem. Phys. 102: 182-188, 2012. Go to original source...
    49. Rout, N.P., Shaw, B.P.: Salt tolerance in aquatic macrophytes: possible involvement of the antioxidative enzymes. - Plant Sci. 160: 415-423, 2001. Go to original source...
    50. Spurr, A.R.: A low viscosity epoxy resin embedding medium for electron microscopy. - J. Ultra Mol. Struct. R. 26: 31-43, 1969. Go to original source...
    51. van Camp, W., Capiau, K., van Montagu, M. et al.: Enhancement of oxidative stress tolerance in transgenic tobacco plants over producing Fe-superoxide dismutase in chloroplasts. - Plant Physiol. 112: 1703-1714, 1996. Go to original source...
    52. Wang, C.R., Wang, X.R., Tian, Y. et al.: Oxidative stress, defense response, and early biomarkers for lead-contaminated soil in Vicia faba seedlings. - Environ. Toxicol. Chem. 27: 970-977, 2008. Go to original source...
    53. Wilcut, J.W., York, A.C., Wehtje, G.R.: The control and interaction of weeds in peanut. - Rev. Weed Sci. 6: 177-205, 1994.
    54. Yin, X.L., Jiang, L., Song, N.H., Yang, H.: Toxic reactivity of wheat (Triticum aestivum) plants to herbicide isoproturon. - J. Agric. Food. Chem. 56: 4825-4831, 2008. Go to original source...
    55. Yu, Q., Friesen, L.J.S., Zhang, X.Q. et al.: Tolerance to acetolactate synthase and acetyl-coenzyme A carboxylase inhibiting herbicides in Vulpia bromoides is conferred by two co-existing resistance mechanisms. - Pestic. Biochem. Phys. 78: 21-30, 2004. Go to original source...
    56. Zhang, X.Q., Powles, S.B.: The molecular bases for resistance to acetyl co-enzyme A carboxylase (ACCase) inhibiting herbicides in two target-based resistant biotypes of annual ryegrass (Lolium rigidum). - Planta 223: 550-557, 2006. Go to original source...

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