Photosynthetica 2018, 56(4):1204-1211 | DOI: 10.1007/s11099-018-0822-8

Effects of exogenous calcium on mesophyll cell ultrastructure, gas exchange, and photosystem II in tobacco (Nicotiana tabacum Linn.) under drought stress

W. Hu1, S. B. Tian2, Q. Di2,*, S. H. Duan3, K. Dai4
1 College of Resources and Environment, Southwest University, Chongqing, China
2 Vegetable and Flower Institute of Chongqing Academy of Agricultural Sciences, Chongqing, China
3 Hunan Tobacco Science Institute, Changsha, China
4 Biological Control of Tobacco Diseases and Insect Pests Engineering Research Center of China Tobacco, Yuxi, China

The effects of calcium chloride solution (10 mmol L-1) on mesophyll cell ultrastructure, gas exchange, chlorophyll and carotenoid content, and PSII in tobacco leaf were studied by simulating water deficit conditions via treatment with 25% PEG-6000 for 24 h. The results showed that under drought stress, the mesophyll cell structure and morphology were destroyed, photosynthesis and gas-exchange processes changed, photosynthetic pigment content decreased, and the electron transfer efficiency in PSII reduced. However, compared with the control treatment, under drought conditions, the addition of exogenous calcium could stabilize the structure and function of the chloroplasts, mitochondria, and endomembrane system in the mesophyll cells, maintain normal leaf net photosynthetic rate and gas exchange, alleviate the degree of photosynthetic pigment degradation, and increase the electron transfer energy in the leaves in PSII. As a means of ensuring normal photosynthesis under drought stress, we discovered that the application of exogenous calcium was more important for stabilization of the structure of the organelles, regulation of the osmotic balance, and increase of the photosynthetic pigment content, and proved to be less important for regulation of stomatal opening and closing.

Additional key words: abiotic stress; chlorophyll a fluorescence; electron transport rate; foliar fertilizer

Received: August 26, 2017; Accepted: February 7, 2018; Prepublished online: December 1, 2018; Published: November 1, 2018  Show citation

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Hu, W., Tian, S.B., Di, Q., Duan, S.H., & Dai, K. (2018). Effects of exogenous calcium on mesophyll cell ultrastructure, gas exchange, and photosystem II in tobacco (Nicotiana tabacum Linn.) under drought stress. Photosynthetica, 56(4), 1204-1211. doi: 10.1007/s11099-018-0822-8
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