biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 42:549-557, 1999 | DOI: 10.1023/A:1002615115118

Effect of Methyl Ester of Jasmonic Acid and Benzylaminopurine on Growth and Protein Profile of Excised Cotyledons of Cucurbita Pepo (Zucchini)

K.I. Ananieva1, E.D. Ananiev1
1 Institute of Plant Physiology "M. Popov", Bulgarian Academy of Sciences, Sofia, Bulgaria E-mail

Treatment of excised marrow (Cucurbita pepo L., zucchini) cotyledons with methyl ester of jasmonic acid (MeJA) had no effect on their growth in darkness. On the other hand, MeJA induced the synthesis of three polypeptides (69, 60 and 43 kDa) and stimulated the accumulation of other polypeptides (97.4 and 53 kDa). These changes in the polypeptide profile were accompanied by a suppression of total protein and RNA synthesis as well as the activity of nuclear RNA polymerases. In contrast to MeJA, N6-benzylaminopurine (BAP) significantly enhanced cotyledon growth and stimulated protein and RNA synthesis. Furthermore, BAP, when applied together with MeJA, was able to counteract some effects of MeJA including the appearance of specific MeJA-induced polypeptide bands.

Keywords: endogenous nuclear RNA polymerases; polyacrylamide gel electrophoresis; protein and RNA synthesis
Subjects: Cucurbita pepo (zucchini); endogenous nuclear RNA polymerases, polypeptides; jasmonic acid methyl ester, growth; marrow cotyledons, growth; polypeptides, nuclear RNA polymerases; protein and RNA synthesis, BAP, jasmonic acid; RNA polymerases, nuclear, polypeptides

Published: December 1, 1999  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Ananieva, K.I., & Ananiev, E.D. (1999). Effect of Methyl Ester of Jasmonic Acid and Benzylaminopurine on Growth and Protein Profile of Excised Cotyledons of Cucurbita Pepo (Zucchini). Biologia plantarum, 42(4), 549-557. doi: 10.1023/A:1002615115118
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ananiev, E.D., Karagyozov, L.K., Karanov, E.N.: Effect of cytokinins on ribosomal RNA gene expression in excised cotyledons of Cucurbita pepo L.-Planta 170: 370-378, 1987. Go to original source...
    2. Ananiev, E.D., Karagyozov, L.K.: Comparative effect of sarkosyl, heparin and aurintricarboxylic acid on RNA polymerase activity in isolated barley nuclei.-Physiol. vÉg. 22: 555-563, 1984.
    3. Ananieva, K.I., Ananiev, E.D.: Methyl jasmonate-induced polypeptides in excised cotyledons of Cucurbita pepo L. (zucchini) and counteraction of cytokinins.-Compt. rend. Acad. bulg. Sci. 51: 3-4, 1998.
    4. Bewley, J.D., Black, M.: Physiology and Biochemistry of Seeds. Vol. 1. Pp. 177-244. Springer-Verlag, Berlin 1978. Go to original source...
    5. Burton, K.: A study on the conditions and the mechanism of the diphenylamine reaction for the colorimetric estimation of DNA.-Biochem. J. 62: 315-323, 1956. Go to original source...
    6. Creelman, R.A., Mullet, J.E.: Biosynthesis and action of jasmonates in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 48: 355-381, 1997. Go to original source...
    7. Dermastia, M., Ravnikar, M., Vilhar, B., Kovac, M.: Increased level of cytokinin ribosides in jasmonic acid-treated potato (Solanum tuberosum) stem node cultures.-Physiol. Plant. 92: 241-246, 1994. Go to original source...
    8. Klyachko N.L., Ananiev, E.D., Kulaeva, O.N.: Effect of 6-benzylaminopurine and abscisic acid on protein synthesis in isolated pumpkin cotyledons.-Physiol. vÉg. 17: 607-617, 1979.
    9. Koda, Y.: The role of jasmonic acid and related compounds in the regulation of plant development.-Int. Rev. Cytol. 135: 155-199, 1992. Go to original source...
    10. Kulaeva, O.N.: Cytokinin action on transcription and translation in plants.-In: Guern, J., PÉaud-Lenoël, C. (ed.): Metabolism and Molecular Activities of Cytokinins. Pp. 218-227. Springer-Verlag, Berlin-Heidelberg-New York 1981. Go to original source...
    11. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 690-85, 1970. Go to original source...
    12. Leshem, B., Ronen, R., Soudry, E., Lurie, S., Gepstein, S.: Cytokinin at a large scale of concentrations determines rates of polypeptide metabolism and regeneration in cultured melon cotyledons.-J. Plant Physiol. 143: 330-336, 1994. Go to original source...
    13. Letham, D.S., Palni, L.M.S.: The biosynthesis and metabolism of cytokinins.-Annu. Rev. Plant Physiol. 34: 163-197, 1983. Go to original source...
    14. Longo, G., Pedretti, M., Rossi, G., Longo C.: Effect of benzyladenine on the development of plastids and microbodies in excised watermelon cotyledons.-Planta 145: 209-217, 1979. Go to original source...
    15. Lowry, H.O., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    16. Mans, R.J., Novelli, C.D.: Measurement of the incorporation of radioactive aminoacids into protein by a filter paper disk method.-Arch. Biochem. Biophys. 94: 48, 1961. Go to original source...
    17. Montague, M.J.: Exogenous jasmonic and abscisic acids act differentially in elongating tissues from oat stem segments.-J. Plant Growth Regul. 16: 11-19, 1997. Go to original source...
    18. Mueller-Uri, F., Parthier, B., Nover, L.: Jasmonate-induced alteration of gene expression in barley leaf segments analyzed by in-vivo and in-vitro protein synthesis.-Planta 176: 241-247, 1988. Go to original source...
    19. Reinbothe, S., Machmudova, A., Wasternack, C., Reinbothe, C., Parthier, B.: Jasmonate-induced proteins in cotton: immunological relationship to the respective barley proteins and homology of transcripts to late embryogenesis abundant (Lea) mRNAs.-J. Plant Growth Regul. 11: 7-14, 1992. Go to original source...
    20. Sano, H., Seo, S., Koizumi, N., Niki, T., Iwamura, H., Ohashi, Y.: Regulation by cytokinins of endogenous levels of jasmonic and salicylic acids in mechanically wounded tobacco plants.-Plant Cell Physiol. 37: 762-769, 1996. Go to original source...
    21. Sembdner, G., Parthier, B.: The biochemistry and the physiological and molecular actions of jasmonates.-Annu. Rev. Plant Physiol. Plant mol. Biol. 44: 569-589, 1993. Go to original source...
    22. Ueda, J., Kato, J., Yamane, H., Takahashi, N.: Inhibitory effect of methyl jasmonate and its related compounds on kinetin induced retardation of oat leaf senescence.-Physiol. Plant 52: 305-309, 1981. Go to original source...
    23. Ueda, J., Kato, J.: Inhibition of cytokinin-induced plant growth by jasmonic acid and its methyl ester.-Physiol. Plant. 54: 249-252, 1982. Go to original source...
    24. Ueda, J., Miyamoto, K., Aoki, M.: Jasmonic acid inhibits the IAA-induced elongation of oat coleoptile segments: A possible mechanism involving the metabolism of cell wall polysaccharides.-Plant Cell Physiol. 35: 1065-1070, 1994.
    25. Wasternack, C., Partier, B.: Jasmonate-signalled plant gene expression.-Trends Plant Sci. 2: 302-307, 1997. Go to original source...
    26. Weidhase, R.A., Lehmann, J., Kramell, H., Sembdner, G., Parthier, B.: Degradation of ribulose-1,5-biphosphate carboxylase and chlorophyll in senescing barley leaf segments triggered by jasmonic acid methylester, and counteraction by cytokinin.-Physiol. Plant. 669: 161-166, 1987a. Go to original source...
    27. Weidhase, R.A., Kramell, H., Lehmann, J., Liebisch, W., Lerbs, H.-W., Parthier, B.: Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments.-Plant Sci. 51: 177-186, 1987b. Go to original source...

    Return to the content