Photosynthetica 1999, 36(4):497-507 | DOI: 10.1023/A:1007031818695

The Development of Chloroplast Ultrastructure and Hill Reaction Activity During Leaf Ontogeny in Different Maize (Zea Mays L.) Genotypes

J. Kutík1, M. Kočova2, D. Holá2, M. Körnerová2
1 Department of Plant Physiology Faculty of Sciences, Charles University, Praha 2, Czech Republic
2 Department of Genetics and Microbiology, Faculty of Sciences, Charles University, Praha 2, Czech Republic

Changes in Hill reaction activity (HRA) and ultrastructure of mesophyll cell (MC) chloroplasts were studied during the ontogeny of third leaf of maize plants using polarographic oxygen evolution measurement, transmission electron microscopy, and stereology. The chloroplast ultrastructure was compared in young (actively growing), mature, and senescing leaves of two different inbreds and their reciprocal F1 hybrids. Statistically significant differences in both HRA and MC chloroplast ultrastructure were observed between different stages of leaf ontogeny. Growth of plastoglobuli was the most striking characteristic of chloroplast maturation and senescence. The chloroplasts in mature and senescing leaves had a more developed system of thylakoids compared to the young leaves. Higher HRA was usually connected with higher thylakoid volume density of MC chloroplasts.

Additional key words: chloroplast dimensions; electron microscopy; peripheral reticulum; photosynthesis; plastoglobuli; starch; stereology; stroma; thylakoids

Prepublished online: January 1, 2000; Published: December 1, 1999  Show citation

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Kutík, J., Kočova, M., Holá, D., & Körnerová, M. (1999). The Development of Chloroplast Ultrastructure and Hill Reaction Activity During Leaf Ontogeny in Different Maize (Zea Mays L.) Genotypes. Photosynthetica, 36(4), 497-507. doi: 10.1023/A:1007031818695
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