biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:149-152, 2003 | DOI: 10.1023/A:1022356322373

Aluminum Effects on Lipid Peroxidation and Antioxidative Enzyme Activities in Rice Leaves

M.C. Kuo1, C.H. Kao1
1 Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

The effects of aluminum on lipid peroxidation and activities of antioxidative enzymes were investigated in detached rice leaves treated with 0 to 5 mM AlCl3 at pH 4.0 in the light. AlCl3 enhanced the content of malondialdehyde but not the content of H2O2. Superoxide dismutase activity was reduced by AlCl3, while catalase and glutathione reductase activities were increased. Peroxidase and ascorbate peroxidase activities were increased only after prolonged treatment, when toxicity occurred. The results give evidence that Al treatment caused oxidative stress and in turn, it caused lipid peroxidation.

Keywords: Oryza sativa; oxidative stress
Subjects: aluminium effects, lipid peroxidation; antioxidative enzymes, aluminium; lipid peroxidation; malondialdehyde; Oryza sativa; rice, aluminum effects, lipid peroxidation

Prepublished online: March 1, 2003; Published: July 1, 2003  Show citation

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Kuo, M.C., & Kao, C.H. (2003). Aluminum Effects on Lipid Peroxidation and Antioxidative Enzyme Activities in Rice Leaves. Biologia plantarum46(1), 149-152. doi: 10.1023/A:1022356322373
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References

  1. Akeson, M.A., Munns, D.N., Burau, R.G.: Adsorption of Al3+ to phosphatidylcholine vesicles.-Biochim. biophys. Acta 986: 33-40, 1989. Go to original source...
  2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
  3. Cakmak, I., Horst, W.J.: Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max).-Physiol. Plant. 83: 463-468, 1991. Go to original source...
  4. Chien, H.-F., Wang, J.-W., Lin, C.C., Kao, C.H.: Cadmium toxicity of rice leaves is mediated through lipid peroxidation.-Plant Growth Regul. 33: 205-313, 2001. Go to original source...
  5. Deleers, M., Servais, J.P., Wülfert, E.: Neurotoxic cations induce membrane rigidification and membrane fusion at micromolar concentrations.-Biochim. biophys. Acta 855: 271-276, 1986. Go to original source...
  6. Ezaki, B., Yamamoto, Y., Matsumoto, H.: Cloning and sequencing of the cDNAs induced by aluminum treatment and Pi starvation in cultured tobacco cells.-Physiol. Plant. 93: 11-18, 1995. Go to original source...
  7. Ezaki, B., Tugita, S., Matsumoto, H.: Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells: possible involvement of peroxidase isozymes in aluminum ion stress.-Physiol. Plant. 96: 21-28, 1996. Go to original source...
  8. Fang, W.-C., Wang, J.-W., Lin, C.C., Kao, C.H.: Iron induction of lipid peroxidation and effects on antioxidative enzyme activities in rice leaves.-Plant Growth Regul. 35: 75-80, 2001. Go to original source...
  9. Foster, J.G., Hess, J.L.: Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to atmosphere enriched in oxygen.-Plant Physiol. 66: 482-487, 1980. Go to original source...
  10. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
  11. Horst, W.J., Asher, C.J., Cakmak, I., Szulkiewica, P., Wissemeier, A.H.: Short-sterm responses of soybean roots to aluminum.-J. Plant Physiol. 140: 174-178, 1992. Go to original source...
  12. Ikegawa, H., Yamamoto, Y., Matsumoto, H.: Responses to aluminum of suspension-cultured tobacco cells in a single cadmium solution.-Soil Sci. Plant Nutr. 46: 503-514, 2000.
  13. Jana, S., Choudhuri, M.A.: Glycolate metabolism of three submerged aquatic angiosperm during aging.-Aquat. Bot. 12: 345-354, 1981. Go to original source...
  14. Kato, M., Shimizu, S.: Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves: phenolic-dependent peroxidative degradation.-Can. J. Bot. 65: 729-735, 1987. Go to original source...
  15. Lin, C.C., Kao, C.H.: Effect of NaCl stress on H2O2 metabolism in rice leaves.-Plant Growth Regul. 30: 151-155, 2000. Go to original source...
  16. Lin, J.-N., Wang, J.-W., Kao, C.H.: Effect of abscisic acid and water stress on the senescence of detached rice leaves.-Biol. Plant. 42: 85-88, 1999. Go to original source...
  17. MacAdam, J.W., Nelson, C.J., Sharp, R.E.: Peroxidase activity in the leaf elongation zone of tall fescue. I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of elongation zone.-Plant Physiol. 99: 872-878, 1992. Go to original source...
  18. Moran, J.F., Becana, M., Iturbe-Ormaetxe, I., Frechilla, S., Klucas, R.V., Aspariciv-Tejo, P.: Drought induces oxidative stress in pea plants.-Planta 194: 346-352, 1994. Go to original source...
  19. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
  20. Ono, K., Yamamoto, Y., Hachiya, A., Matsumoto, H.: Synergistic inhibition of growth by aluminum and iron of tobacco (Nicotian tobacum L.) cells in suspension culture.-Plant Cell Physiol. 36: 115-125, 1995.
  21. Oteiza, P.I.: A mechanism for the stimulatory effect of aluminum on iron-induced lipid peroxidation.-Arch. Biochem. Biophys. 308: 374-379, 1994. Go to original source...
  22. Paoletti, F., Aldinucci, D., Mocali, A., Capparini, A.: A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts.-Anal. Biochem. 154: 536-541, 1986. Go to original source...
  23. Richards, K.D., Schott, E.J., Sharma, Y.K., Davis, K.R., Gardner, R.C.: Aluminum induces oxidative stress genes in Arabidopsis thaliana.-Plant Physiol. 116: 409-418, 1998. Go to original source...
  24. Sakihama, Y., Yamasaki, H.: Lipid peroxidation induced by phenolics in conjunction with aluminum ions.-Biol. Plant. 45: 249-254, 2002. Go to original source...
  25. Snowden, K.C., Gardner, R.C.: Five genes induced by aluminum in wheat (Triticum aestivum L.) roots.-Plant Physiol. 103: 855-861, 1993. Go to original source...
  26. Thompson, J.E., Legge, R.L., Barber, R.F.: The role of free radicals in senscence and wounding.-New Phytol. 105: 317-344, 1987. Go to original source...
  27. Wintermans, J.F.G.M., De Mots, A.: Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol.-Biochim. biophys. Acta 109: 448-453, 1965. Go to original source...
  28. Yamaguchi, Y., Yamamoto, Y., Matsumoto, H.: Cell death process initiated by a combination of aluminum and iron in suspension-cultured tobacco cells (Nicotiana tabacum): apoptosis-like cell death mediated by calcium and proteinase.-Soil Sci. Plant Nutr. 45: 647-657, 1999. Go to original source...
  29. Yamamoto, Y., Hachiya, A., Matsumoto, H.: Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells.-Plant Cell Physiol. 38: 1333-1339, 1997. Go to original source...
  30. Yamamoto, Y., Kobayashi, Y., Matsumoto, H.: Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots.-Plant Physiol. 125: 199-208, 2001. Go to original source...