biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:275-279, 2003 | DOI: 10.1023/A:1022867030790

Water Stress Induced Proline Accumulation in Contrasting Wheat Genotypes as Affected by Calcium and Abscisic Acid

H. Nayyar1, D.P. Walia2
1 Department of Botany, Panjab University, Chandigarh, India
2 Department of Fruit Breeding and Genetic Resources, U.H.F., Nauni, Solan, India

Proline accumulation and mobilization in roots of 7-d-old seedlings of wheat genotypes varying in sensitivity towards water stress were compared. Water stress was induced by polyethylene glycol (PEG-6000; osmotic potential -1.5 MPa) in the presence of 0.1 mM abscisic acid (ABA), 1 mM calcium chloride, 0.5 mM verapamil (Ca2+ channel blocker), 0.5 mM fluridone (inhibitor of ABA biosynthesis). While both the genotypes did not differ in total proline accumulation, rate of proline accumulation and utilization was higher in tolerant genotype C 306 as compared to susceptible genotype HD 2380. The treatment with ABA and CaCl2 caused further increase in proline accumulation during stress and reduced its mobilization during recovery. The membrane stability and elongation rate of roots was observed to be higher at ABA and calcium treatment in both the genotypes under stress. As was evident from inhibitor studies, the tolerant genotype was more responsive to ABA and the susceptible one to calcium.

Keywords: apparent proline mobilization rate; fluridone; membrane stability index; root elongation rate; Triticum aestivum; verapamil
Subjects: abscisic acid (ABA); amino acids; apparent proline mobilization rate; calcium, proline accumulation, ABA, water stress; fluridon; membrane stability index; proline accumulation, calcium, ABA, water stress; root elongation; Triticum aestivum; verapamil; water stress, proline accumulation; wheat, genotypes, water stress, proline accumulation

Prepublished online: March 1, 2003; Published: September 1, 2003  Show citation

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Nayyar, H., & Walia, D.P. (2003). Water Stress Induced Proline Accumulation in Contrasting Wheat Genotypes as Affected by Calcium and Abscisic Acid. Biologia plantarum, 46(2), 275-279. doi: 10.1023/A:1022867030790
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