biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 48:211-218, 2004 | DOI: 10.1023/B:BIOP.0000033447.27149.85

Cell Cycle Activity and β-Tubulin Accumulation During Dormancy Breaking of Acer platanoides L. seeds

T.A. Paw³owski1,*, J.H.W. Bergervoet2, R.J. Bino2, S.P.C. Groot2
1 Institute of Dendrology, Polish Academy of Sciences, Kórnik, Poland
2 Plant Research International, Plant Development and Reproduction, Wageningen, The Netherlands

Cell cycle events in embryo axes of Norway maple (Acer platanoides L.) seeds were studied during dormancy breaking by flow cytometric analyses of the nuclear DNA content and by immunodetection of β-tubulin. Most embryonic nuclei of dry, fully matured seeds were arrested in the G2 phase of the cell cycle. In addition, the lowest content of β-tubulin was detected in dry, mature seeds. Imbibition in water and cold stratification resulted in a decrease in the number of nuclei in G2, and a simultaneous increase in β-tubulin content. In germinated seeds the content of β-tubulin was the highest and the number of cells in G2 was the lowest. Both cell cycle events preceded cell expansion and division and subsequent growth of the radicle through the seed coat. The anatomical investigation has proved that the main reason for decrease in the number of nuclei in G2 is mitosis, started with seeds germination (radicle protrusion). The activation of the cell cycle and the β-tubulin accumulation were associated with embryo dormancy breaking.

Keywords: development; DNA; flow cytometry; germination; microtubule; Norway maple
Subjects: Acer platanoides; cell cycle; dormancy breaking; flow cytometry; germination; growth analysis, plant development, biomass and yield enhancement; microtubule; Norway maple, dormancy; seed development, germination; seed, dormancy; β-tubulin

Published: June 1, 2004  Show citation

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Paw³owski, T.A., Bergervoet, J.H.W., Bino, R.J., & Groot, S.P.C. (2004). Cell Cycle Activity and β-Tubulin Accumulation During Dormancy Breaking of Acer platanoides L. seeds. Biologia plantarum, 48(2), 211-218. doi: 10.1023/B:BIOP.0000033447.27149.85
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    References

    1. Argerich, C.A., Bradford, K.J.: The effects of priming and ageing on seed vigour in tomato.-J. exp. Bot. 40: 509-607, 1989. Go to original source...
    2. Baiza, A., Aguilar, R., de Jimenez, S.E.: Histones and the first cell cycle in maize germination.-Physiol. Plant. 68: 259-264, 1986. Go to original source...
    3. Balu¹ka, F., Barlow, P.W.: The role of the microtubular cytoskeleton in determining nuclear chromatin structure and passage of maize root cells through the cell cycle.-Eur. J. Cell Biol. 61: 160-167, 1993.
    4. Barlow, P.W.: The cell division cycle in relation to root organogenesis.-In: Ormrod, J.C., Francis, D. (ed.): Molecular and Cellular Biology of the Plant Cell Cycle. Pp. 179-199. Kluver Academic Publishers, Dordrecht 1993. Go to original source...
    5. Bassi, P.: Quantitative variations of nuclear DNA during plant development: a critical analysis.-Biol. Rev. 65: 185-225, 1990. Go to original source...
    6. Bergervoet, J.H.W., Jing, H.C., Van den Hout, J.W.E., De Castro, R.D., Kunneman, B.P.A.M., Bino, R.J., Groot, S.P.C.: Expression of beta-tubulin during dormancy induction and release in apical and axillary buds of five woody species.-Physiol. Plant. 106: 238-245, 1999. Go to original source...
    7. Bewley, J.D., Black, M.: Seeds: Physiology of Development and Germination. 2nd Ed.-Plenum Press, New York 1994. Go to original source...
    8. Bino, R.J., De Vries, J.N., Kraak, H.L., Van Pijlen, J.G.: Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination.-Ann. Bot. 69: 231-236, 1992. Go to original source...
    9. Bino, R.J., Lanteri, S., Verhoeven, H.A., Kraak, H.L.: Flow cytometric determination of nuclear replication stages in seed tissues.-Ann. Bot. 72: 181-187, 1993. Go to original source...
    10. Clayton, L.: The cytoskeleton and the plant cell cycle.-In: Bryant, J.A., Francis, D. (ed.): The Cell Division Cycle in Plants. Pp. 113-131. Cambridge University Press, Cambridge 1985.
    11. Dandoy, E., Schyns, R., Deltour, R., Verly, W.G.: Appearance and repair of apurinic/apyrimidinic sites in DNA during early germination of Zea mays.-Mutat. Res. 181: 57-60, 1987. Go to original source...
    12. De Castro, R.D., Zheng, X., Bergervoet, J.H.W., De Vos, R.C.H., Bino, R.J.: β-tubulin accumulation and DNA replication in imbibing tomato seeds.-Plant Physiol. 109: 499-504, 1995. Go to original source...
    13. Deltour, R.: Nuclear activation during early germination of the higher plant embryo.-J. Cell Sci. 75: 43-83, 1985. Go to original source...
    14. Finch-Savage, W.E., Bergervoet, J.H.W., Bino, R.J., Clay, H.A., Groot, S.P.C.: Nuclear replication activity during seed development, dormancy breakage and germination in three tree species: Norway maple (Acer platanoides L.), sycamore (Acer pseudoplatanus L.) and cherry (Prunus avium L.).-Ann. Bot. 81: 519-526, 1998. Go to original source...
    15. Górnik, K., De Castro, R.D., Liu, Y., Bino, R.J., Groot, S.P.C. Inhibition of cell division during cabbage (Brassica oleracea L.) seed germination.-Seed Sci. Res. 7: 333-340, 1997. Go to original source...
    16. Gunning, B.E.S., Sammut, M.: Rearrangement of microtubules involved in establishing cell division planes start immediately after DNA synthesis and are completed just before mitosis.-Plant Cell 2: 1273-1282, 1990. Go to original source...
    17. Hepler, P.K., Hush, J.M.: Behavior of microtubules in living plant cells.-Plant. Physiol. 112: 455-461, 1996. Go to original source...
    18. Hodurková, J., Vyskot, B.: Histone H4 acetylation patterns during seed germination and early plant development.-Biol. Plant. 46: 23-28, 2003. Go to original source...
    19. Hussey, P.J., Haas, N., Hunsperger, J., Larkin, J., Snustad, D.P., Silflow, C.D.: The β-tubulin family in Zea mays: two differentially expressed β-tubulin genes.-Plant mol. Biol. 15: 957-972, 1990. Go to original source...
    20. Hussey, P.J., Lloyd, C.W., Gull, K.: Differential developmental expression of β-tubulin in a higher plant.-J. biol. Chem. 263: 5474-5479, 1988. Go to original source...
    21. Jarvis, S.B., Taylor, M.A., Bianco, J., Corbineau, F., Davies, H.V.: Dormancy-breakage in seeds of Douglas fir (Pseudotsuga menziesii (Mirb.) Franco). Support for the hypothesis that LEA gene expression is essential for this process.-J. Plant Physiol. 151: 457-464, 1997. Go to original source...
    22. Kudo, N., Kimura, Y.: Patterns of endopolyploidy during seedling development in cabbage (Brassica oleracea L.).-Ann. Bot. 87: 275-281, 2001. Go to original source...
    23. Lanteri, S., Kraak, H.L., De Vos, C.H.R., Bino, R.J.: Effects of osmotic preconditioning on nuclear replication activity in seeds of pepper (Capsicum annuum).-Physiol. Plant. 89: 433-440, 1993. Go to original source...
    24. Liu, Y., Hilhorst, H.W.M., Groot, S.P.C., Bino, R.J.: Amounts of nuclear DNA and internal morphology of gibberellinand abscisic acid-deficient tomato (Lycopersicon esculentum Mill.) seeds during maturation, imbibition and germination.-Ann. Bot. 79: 161-168, 1997. Go to original source...
    25. Mullen, R.T., King, J.E., Gifford, D.J.: Changes in mRNA populations during loblolly pine (Pinus taeda) seed stratification, germination and post-germinative growth.-Physiol. Plant. 97: 545-553, 1996. Go to original source...
    26. Paw³owski T., Kalinowski A. Qualitative changes in the proteins of Acer platanoides seeds during maturation.-Acta biol. cracov. Bot. 42: 145-150, 2003.
    27. Paw³owski, T., Szczotka, Z.: Qualitative changes in protein content during cold and warm stratification of Norway maple (Acer platanoides L.) seeds.-Seeds Sci. Res. 7: 385-390, 1997. Go to original source...
    28. Paw³owski, T., Szczotka, Z.: Qualitative changes in the proteins of cotyledons during cold and warm stratification of Acer platanoides seeds.-Acta Soc. Bot. Pol. 70: 17-23, 2001. Go to original source...
    29. Paw³owski, T., Szczotka, Z., Krawiarz, K.: Qualitative changes and dynamic of protein synthesis during cold and warm stratification of Norway maple (Acer platanoides L.) seeds.-Acta Soc. Bot. Pol. 66: 333-341, 1997.
    30. Raghavan, V.: Molecular Embryology of Flowering Plants. 1st Ed.-Cambridge University Press, New York 1997. Go to original source...
    31. Ross, J.D.: Dormancy breakage by chilling: phytochrome, calcium and calmodulin.-In: Lang, G.A. (ed.): Plant Dormancy: Physiology, Biochemistry and Molecular Biology. Pp. 157-170. CAB International, Wellingford 1996.
    32. Ross, J.D., Bradbeer, J.W.: Studies in seed dormancy.-Planta 100: 288-302, 1971. Go to original source...
    33. Satoh, S., Esashi, Y.: Protein synthesis in dormant and nondormant cocklebur seed segments.-Physiol. Plant. 47: 229-234, 1979. Go to original source...
    34. Sgorbati, S., Sparvoli, E., Levi, M., Chiatante, D.: Bivariate cytofluorimetric analysis of nuclear protein and DNA relative to cell kinetics during germination of Pisum sativum seed.-Physiol. Plant. 75: 479-484, 1989. Go to original source...
    35. Slater, R.J., Bryant, J.A.: RNA polymerase activity during breakage of seed dormancy by low temperature treatment of fruits of Acer platanoides (Norway maple).-J. exp. Bot. 38: 1026-1032, 1987. Go to original source...
    36. Szczotka, Z., Tomaszewska, E.: Some metabolic processes accompanying dormancy breaking in the seeds of Norway maple (Acer platanoides L.).-Arb. kórnickie 24: 137-145, 1979.
    37. Taylor, M.A., Davies, H.V., Smith, S.B., Abruzzese, A., Gosling, P.G.: Cold-induced changes in gene expression during dormancy breakage in seeds of Douglas-fir (Pseudotsuga menziesii).-J. Plant Physiol. 142: 120-123, 1993. Go to original source...
    38. Tetteroo, F.A.A., Bergervoet, J.H.W., Hasenack, B.: Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids.-Physiol. Plant. 95: 154-158, 1995. Go to original source...
    39. Tomaszewska, E.: Growth regulators in Norway maple (Acer platanoides L.) seeds.-Arb. kórnickie 25: 189-196, 1976.
    40. Wareing, P.F.: Hormonal regulation of seed dormancy-past, present and future.-In: Khan, A.A. (ed.): The Physiology and Biochemistry of Seeds Development, Dormancy and Germination. Pp. 185-202. Elsevier Biomedical Press, Amsterdam 1982.

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