Photosynthetica 2004, 42(3):447-455 | DOI: 10.1023/B:PHOT.0000046165.15048.a4

Ultrastructure and Dimensions of Chloroplasts in Leaves of Three Maize (Zea mays L.) Inbred Lines and their F1 Hybrids Grown Under Moderate Chilling Stress

J. Kutík1, D. Holá2, M. Kočová2, O. Rothová2, D. Haisel3, N. Wilhelmová3, I. Tichá1
1 Department of Plant Physiology, Faculty of Science, Charles University in Prague, Czech Republic
2 Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Czech Republic
3 Laboratory of Stress Physiology, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, cNa Karlovce 1a, CZ, Czech Republic

Influence of moderate chilling stress on vascular bundle sheath cell (BSC) and especially mesophyll cell (MC) chloroplasts of mature maize leaves was studied by electron microscopy and stereology. Plants of two inbred lines of maize, differing in their photosynthetic activity, and their F1 hybrids were cultivated during autumn in heated or unheated glasshouse. Generally, chilling temperatures resulted mainly in the decrease in stereological volume density (VD) of both granal and intergranal thylakoids of MC chloroplasts, while the ratio of granal to all thylakoids (granality) was less affected. The VD of peripheral reticulum and plastoglobuli usually increased after cold treatment of plants. The volume of MC chloroplasts usually increased under chilling stress, the shape of the chloroplasts changed only slightly. The ultra-structure of chloroplasts differed between individual genotypes; chilling-stressed hybrid plants showed positive heterosis particularly in the granal thylakoids' VD of MC chloroplasts.

Additional key words: bundle sheath cells; electron microscopy; genotypes; heterosis; intra-specific variability; mesophyll cells; peripheral reticulum; plastoglobuli; stereology; thylakoids

Published: September 1, 2004  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Kutík, J., Holá, D., Kočová, M., Rothová, O., Haisel, D., Wilhelmová, N., & Tichá, I. (2004). Ultrastructure and Dimensions of Chloroplasts in Leaves of Three Maize (Zea mays L.) Inbred Lines and their F1 Hybrids Grown Under Moderate Chilling Stress. Photosynthetica, 42(3), 447-455. doi: 10.1023/B:PHOT.0000046165.15048.a4
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arvidsson, P.-O., Sundby, C.: A model for topology of the chlo-roplast thylakoid membrane. - Aust. J. Plant Physiol. 26: 687-694, 1999. Go to original source...
    2. Biswal, B., Biswal, U.C.: Photosynthesis under stress: stress signals and adaptive response of chloroplasts. - In: Pessarakli, M. (ed.): Handbook of Plant and Crop Stress. 2 nd Ed. Pp. 315-336. Marcel Dekker, New York - Basel 1999. Go to original source...
    3. Bongard-Pierce, D.K., Evans, M.M.S., Poethig, R.S.: Hetero-blastic features of leaf anatomy in maize and their genetic re-gulation. - Int. J. Plant Sci. 157: 331-340, 1996 Go to original source...
    4. É atský, J., ©esták, Z.: Photosynthesis during leaf development. - In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 633-660. Marcel Dekker, New York - Basel - Hong Kong 1997.
    5. É iamporová, M., Trgi ò ová, I.: Ultrastructure of chloroplasts in leaves and of plastids in root tips of two maize lines differing in chilling tolerance. - Biológia (Bratislava) 51: 441-447, 1996.
    6. É iamporová, M., Trgi ò ová, I.: Modifications of plant cell ultra-structure accompanying metabolic responses to low temperatures. - Biológia (Bratislava) 54: 349-360, 1999.
    7. Du, Y.-C., Nose, A., Wasano, K.: Effects of chilling temperatu-re on photosynthetic rates, photosynthetic enzyme activities and metabolite levels in leaves of three sugarcane species. - Plant Cell Environ. 22: 317-324, 1999. Go to original source...
    8. Fracheboud, Y., Haldimann, P., Leipner, J., Stamp, P.: Chloro-phyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.). - J. exp. Bot. 50: 1533-1540, 1999. Go to original source...
    9. Fracheboud, Y., Ribaut, J.-M., Vargas, M., Messmer, R., Stamp, P.: Identification of quantitative trait loci for cold-tole-rance of photosynthesis in maize (Zea mays L.). - J. exp. Bot. 53: 1967-1977, 2002. Go to original source...
    10. Haldimann, P.: Effect of changes in growth temperature on pho-tosynthesis and carotenoid composition in Zea mays leaves. - Physiol. Plant. 97: 554-562, 1996. Go to original source...
    11. Holá, D., Ko É ová, M., Körnerová, M., Sofrová, D., Sopko, B.: Genetically based differences in photochemical activities of isolated maize (Zea mays L.) mesophyll chloroplasts. - Photo-synthetica 36: 187-197, 1999. Go to original source...
    12. Holá, D., Langrová, K., Ko É ová, M., Rothová, O.: Photosyn-thetic parameters of maize (Zea mays L.) inbred lines and F1 hybrids: their different response to, and recovery from rapid or gradual onset of low-temperature stress. - Photosynthetica 41: 429-442, 2003. Go to original source...
    13. Hudák, J.: Photosynthetic apparatus. - In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 27-48. Marcel Dekker, New York - Basel - Hong Kong 1997.
    14. Hudák, J., Salaj, J.: The effect of low temperatures on the struc-ture of plant cells.-In: Pessarakli, M. (ed.): Handbook of Plant and Crop Stress. 2 nd Ed. Pp. 441-464. Marcel Dekker, New York - Basel 1999. Go to original source...
    15. Körnerová, M., Holá, D.: The effect of low growth temperature on Hill reaction and photosystem 1 activities and pigment contents in maize inbred lines and their F1 hybrids. - Photo-synthetica 37: 477-488, 1999. Go to original source...
    16. Kratsch, H.A., Wise, R.R.: The ultrastructure of chilling stress. - Plant Cell Environ. 23: 337-350, 2000. Go to original source...
    17. Kutík, J.: The development of chloroplast structure during leaf ontogeny. - Photosynthetica 35: 481-505, 1998. Go to original source...
    18. Kutík, J., Holá, D., Vi É ánková, A., ©mídová, M., Ko É ová, M., Körnerová, M., Kubínová, L.: The heterogeneity of structural and functional photosynthetic characteristics of mesophyll chloroplasts in various parts of mature or senescing leaf blade of two maize (Zea mays L.) genotypes. - Photosynthetica 39: 497-506, 2001. Go to original source...
    19. Kutík, J., Ko É ová, M., Holá, D., Körnerová, M.: The develop-ment of chloroplast ultrastructure and Hill reaction activity during leaf ontogeny in different maize (Zea mays L.) genotypes. - Photosynthetica 36: 497-507, 1999. Go to original source...
    20. Lidon, F.C., Loureiro, A.S., Vieira, D.E., Bilho, E.A., Nobre, P., Costa, R.: Photoinhibition in chilling stressed wheat and maize. - Photosynthetica 39: 161-166, 2001. Go to original source...
    21. Mostowska, A.: Environmental factors affecting chloroplasts. - In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 407-426. Marcel Dekker, New York - Basel - Hong Kong 1997.
    22. Mustárdy, L., Garab, G.: Granum revisited. A three-dimensional model - where things fall into place. - Trends Plant Sci. 8: 117-122, 2003. Go to original source...
    23. Nie, G.Y., Baker, N.R.: Modifications to thylakoid composition during development of maize leaves at low growth temperatures. - Plant Physiol. 95: 184-191, 1991. Go to original source...
    24. Nie, G.Y., Robertson, E.J., Fryer, M.J., Leech, R.M., Baker, N.R.: Response of the photosynthetic apparatus in maize lea-ves grown at low temperature on transfer to normal growth temperature. - Plant Cell Environ. 18: 1-12, 1995. Go to original source...
    25. Pastori, G., Foyer, C.H., Mullineaux, P.: Low temperature-in-duced changes in the distribution of H2O2 and antioxidants be-tween the bundle sheath and mesophyll cells of maize leaves. - J. exp. Bot. 51: 107-113, 2000. Go to original source...
    26. Pinhero, R.G., Paliyath, G., Yada, R.Y., Murr, D.P.: Chloroplast membrane organization in chilling-tolerant and chilling-sensi-tive maize seedlings. - J. Plant Physiol. 155: 691-698, 1999. Go to original source...
    27. Ritchie, S.W., Hanway, J.J., Benson, G.O.: How a corn plant develops. - Spec. Rep. No. 48. Iowa State University Exten-sion, Ames 1993.
    28. Robertson, E.J., Baker, N.R., Leech, R.M.: Chloroplast thyla-koid protein changes induced by low growth temperature in maize revealed by immunocytology. - Plant Cell Environ. 16: 809-818, 1993. Go to original source...
    29. Rogers, R.A., Dunn, J.H., Nelson, C.J.: Photosynthesis and cold hardening in Zoysia and bermudagrass. - Crop Sci. 17: 727-732, 1977. Go to original source...
    30. Slack, C.R., Roughan, P.G., Bassett, H.C.M.: Selective inhibi-tion of mesophyll chloroplast development in some C4-path-way species by low night temperature. - Planta 118: 57-73, 1974. Go to original source...
    31. Sopher, C.R., Krol, M., Huner, N.P.A., Moore, A.E., Fletcher, R.A.: Chloroplastic changes associated with paclobutrazol-in-duced stress protection in maize seedlings. - Can. J. Bot. 77: 279-290, 1999. Go to original source...
    32. Tichá, I., Haisel, D., Holá, D., Ko É ová, M., Kutík, J., Rothová, O., Wilhelmová, N.: Chloroplast structure and functions as affected by chilling temperatures. - In: ELSO 2003 Pro-ceedings. P. 127. Dresden 2003.
    33. Vega, S.H., Sauer, M., Orkwiszewski, J.A.J., Poethig, R.S.: The early phase change gene in maize. - Plant Cell 14: 133-147, 2002. Go to original source...
    34. Verheul, M.J., Picatto, C., Stamp, P.: Growth and development of maize (Zea mays L.) seedlings under chilling conditions in the field. - Eur. J. Agron. 5: 31-43, 1996. Go to original source...
    35. Vi É ánková, A., Holá, D., Ko É ová, M., Wilhelmová, N., Haisel, D., Kutík, J.: Changes in structural and photochemical charac-teristics of maize chloroplasts during diurnal cycle and along the leaf blade. - In: Roubelakis-Angelakis, K.A. (ed.): 13 th Congress of the Federation of European Societies of Plant Physiology. Book of Abstracts. P. 483. FESPP, Herakleion 2002.

    Return to the content