biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:179-183, 2000 | DOI: 10.1023/A:1002731605694

In Vitro Preservation of Asparagus Officinalis

S.A. Bekheet1
1 Plant Cell and Tissue Culture Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Egypt

A simple systems for in vitro storage of health asparagus germplasm was developed. High percent (90 %) of shoots cultured in a standard multiplication medium were maintained viable in vitro at 5 °C in darkness for 12 months. This percent was decreased to 60 % when cultures were stored for 18 months. At normal temperature, shoots and callus cultures also survived for 1 year under osmotic stress on medium containing 40 g dm-3 mannitol.

Keywords: cryopreservation; mannitol; osmotic stress
Subjects: Asparagus officinalis; in vitro culture, cryopreservation; osmotic stress, in vitro culture

Published: August 1, 2000  Show citation

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Bekheet, S.A. (2000). In Vitro Preservation of Asparagus Officinalis. Biologia plantarum, 43(2), 179-183. doi: 10.1023/A:1002731605694
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