Photosynthetica 2000, 38(2):221-226 | DOI: 10.1023/A:1007269931946

Increase in Resistance to Low Temperature Photoinhibition Following Ascorbate Feeding is Attributable to an Enhanced Xanthophyll Cycle Activity in Rice (Oryza sativa L.) Leaves

C. C. Xu1, R. C. Lin1, L. B. Li1, T. Y. Kuang2
1 Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing, China
2 Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing, China

The mechanistic basis for protection of exogenous ascorbate against photoinhibition at low temperature was examined in leaves of rice (Oryza sativa L.). Exposure of intact leaves to chilling temperature resulted in a drastic decrease in the speed of development of non-photochemical fluorescence quenching (NPQ). This was related to the low temperature-imposed restriction on the formation of the fast relaxing component of NPQ (qf). Feeding with 20 mM ascorbate markedly increased the rate of qf development at chilling temperature due primarily to the enhanced rate of zeaxanthin (Z) formation. On the other hand, ascorbate feeding had no influence on photosystem 2 (PS2)-driven electron flow. The reduced state of the PS2 primary electron acceptor QA decreased in ascorbate-fed leaves exposed to high irradiance at chilling temperature owing to the increased Z-associated thermal energy dissipation in the light-harvesting antenna system of PS2. Furthermore, ascorbate feeding increased the photosynthetic apparatus of rice leaves to resist photoinhibition at low temperature. The protective effect of exogenous ascorbate was fully accounted for by the enhanced xanthophyll cycle activity.

Additional key words: antheraxanthin; chilling; chlorophyll fluorescence; high irradiance; photochemical quenching; thermal energy dissipation; violaxanthin; zeaxanthin

Prepublished online: November 1, 2000; Published: August 1, 2000  Show citation

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Xu, C.C., Lin, R.C., Li, L.B., & Kuang, T.Y. (2000). Increase in Resistance to Low Temperature Photoinhibition Following Ascorbate Feeding is Attributable to an Enhanced Xanthophyll Cycle Activity in Rice (Oryza sativa L.) Leaves. Photosynthetica, 38(2), 221-226. doi: 10.1023/A:1007269931946
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    References

    1. Adams, W.W., III, Hoehn, A., Demmig-Adams, B.: Chilling temperatures and the xanthophyll cycle. A comparison of warm-grown and overwintering spinach.-Aust. J. Plant Physiol. 22: 75-85, 1995. Go to original source...
    2. Anderson, J.M., Park, Y.-I., Chow, W.S.: Photoinactivation and photoprotection of photosystem II in nature.-Physiol. Plant. 100: 214-223, 1997. Go to original source...
    3. Arvidsson, P.-O., Carlsson, M., Stefánsson, H., Albertsson, P.-A., Åkerlund, H.-E.: Violaxanthin accessibility and temperature dependency for de-epoxidation in spinach thylakoid membranes.-Photosynth. Res. 52: 39-48, 1997. Go to original source...
    4. Asada, K.: Radical production and scavenging in the chloroplasts.-In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 123-150. Kluwer Academic Publishers, Dordrecht-Boston-London 1996. Go to original source...
    5. Biehler, K., Fock, H.: Evidence for the contribution of the Mehler-peroxidase reaction in dissipating excess electrons in drought-stressed wheat.-Plant Physiol. 112: 265-272, 1996. Go to original source...
    6. Bilger, W., Björkman, O.: Temperature dependence of violaxanthin de-epoxidation and non-photochemical fluorescence quenching in intact leaves of Gossypium hirsutum L. and Malva parviflora L.-Planta 184: 226-234, 1991. Go to original source...
    7. Bratt, C.E., Arvidsson, P.-O., Carlsson, M., Åkerlund, H.-E.: Regulation of violaxanthin de-epoxidase activity by pH and ascorbate concentration.-Photosynth. Res. 45: 169-175, 1995. Go to original source...
    8. Brüggemann, W.: Low-temperature limitations of photosynthesis in three tropical Vigna species: A chlorophyll fluorescence study.-Photosynth. Res. 34: 301-310, 1992. Go to original source...
    9. Brüggemann, W., Koroleva, O.Y.: Chilling sensitivity of violaxanthin de-epoxidation inhibits the development of energy-dependent chlorophyll fluorescence quenching in vivo.-Plant Physiol. Biochem. 33: 251-259, 1995.
    10. Brüggemman, W., Linger, P.: Long-term chilling of young tomato plants under low light. IV. Differential responses of chlorophyll fluorescence quenching coefficients in Lycopersicon species of different chilling sensitivity.-Plant Cell Physiol. 35: 585-591, 1994. Go to original source...
    11. Calatayud, A., Deltoro, V.I., Abadía, J., Abadía, J., Barreno, E.: Effects of ascorbate feeding on chlorophyll fluorescence and xanthophyll cycle components in the lichen Parmelia quercina (Willd.) Vainio exposed to atmospheric pollutants.-Physiol. Plant. 105: 679-684, 1999. Go to original source...
    12. Demmig-Adams, B., Adams, W.W., III: The role of xanthophyll cycle carotenoids in the protection of photosynthesis.-Trends Plant Sci. 1: 21-26, 1996. Go to original source...
    13. Eskling, M., Arvidsson, P.-O., Åkerlund, H.-E.: The xanthophyll cycle, its regulation and components.-Physiol. Plant. 100: 806-816, 1997. Go to original source...
    14. Forti, G., Barbagallo, R.P., Inversini, B.: The role of ascorbate in the protection of thylakoids against photoinactivation.-Photosynth. Res. 59: 215-222, 1999. Go to original source...
    15. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    16. Gilmore, A.M.: Mechanistic aspects of xanthophyll cycle-dependent photoprotection in higher plant chloroplasts and leaves.-Physiol. Plant. 99: 197-209, 1997. Go to original source...
    17. Gussakovsky, E.E., Barzda, V., Shahak, Y., Garab, G.: Irreversible disassembly of chiral macrodomains in thylakoids due to photoinhibition.-Photosynth. Res. 51: 119-126, 1997. Go to original source...
    18. Haehnel, W.: Photosynthetic electron transport in higher plants.-Annu. Rev. Plant Physiol. 35: 659-693, 1984. Go to original source...
    19. Hager, A.: Lichtbedingte pH-Erniedrigung in einem Chloroplasten-Kompartiment als Ursache der enzymatischen Violaxanthin→Zeaxanthin-Umwandlung; Beziehungen zur Photophosphorylierung.-Planta 89: 224-243, 1969. Go to original source...
    20. Haldimann, P.: Low growth temperature-induced changes to pigment composition and photosynthesis in Zea mays genotypes differing in chilling sensitivity.-Plant Cell Environ. 21: 200-208, 1998. Go to original source...
    21. Havaux, M.: Effects of chilling on the redox state of the primary electron acceptor QA of photosystem II in chilling-sensitive and resistant plant species.-Plant Physiol. Biochem. 125: 735-743, 1987.
    22. Horton, P.: Are grana necessary for regulation of light harvesting?-Aust. J. Plant Physiol. 26: 659-669, 1999. Go to original source...
    23. Horton, P., Ruban, A.V., Walters, R.G.: Regulation of light harvesting in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655-684, 1996. Go to original source...
    24. Huner, N.P.A., Öquist G., Sarhan, F.: Energy balance and acclimation to light and cold.-Trends Plant Sci. 3: 224-230, 1998. Go to original source...
    25. Istokovics, A., Simidjiev, I., Lajkó, F., Garab, G.: Characterization of the light induced reversible changes in the chiral macroorganization of the chromophores in chloroplast thylakoid membranes. Temperature dependence and effect of inhibitors.-Photosynth. Res. 54: 45-53, 1997. Go to original source...
    26. Johnson, G.N., Young, A.J., Scholes, J.D., Horton, P.: The dissipation of excess excitation energy in British plant species.-Plant Cell Environ. 16: 673-679, 1993. Go to original source...
    27. Jung, S., Steffen, K.L., Lee, H.J.: Comparative photoinhibition of a high and a low altitude ecotype of tomata (Lycopersicon hirsutum) to chilling stress under high and low light conditions.-Plant Sci. 134: 69-77, 1998. Go to original source...
    28. Krause, G.H.: Photoinhibition induced by low temperatures.-In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis: From Molecular Mechanisms to the Field. Pp. 331-348. Bios Scientific Publishers, Oxford 1994.
    29. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    30. Leipner, J., Frachebound, Y., Stamp, F.P.: Acclimation by suboptimal growth temperature diminishes photooxidative damage in maize leaves.-Plant Cell Environ. 20: 366-372, 1997. Go to original source...
    31. Neubauer, C., Yamamoto, H.Y.: Role of ascorbate in the related ascorbate peroxidase, violaxanthin de-epoxidase and non-photochemical fluorescence-quenching activities.-In Yamamoto, H.Y., Smith, C.M. (ed.): Photosynthetic Responses to the Environment. Pp. 166-171. American Society of Plant Physiologists, Rockville 1993.
    32. Park, Y.-I., Chow, W.S., Osmond, C.B., Anderson, J.M.: Electron transport to oxygen mitigates against the photoinactivation of Photosystem II in vivo.-Photosynth. Res. 50: 23-32, 1996. Go to original source...
    33. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    34. Richter, M., Rühle, W., Wild, A.: Studies on the mechanism of photosystem II photoinhibition. II. The involvement of toxic oxygen species.-Photosynth. Res. 24: 237-243, 1990. Go to original source...
    35. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis.-In Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-70. Springer-Verlag, Berlin 1994. Go to original source...
    36. Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331-343, 1990. Go to original source...
    37. Xu, C.-C., Jeon, Y.A., Lee, C.-H.: Relative contributions of photochemical and non-photochemical routes to excitation energy dissipation in rice and barley illuminated at a chilling temperature.-Physiol. Plant. 107: 447-453, 1999. Go to original source...
    38. Yamamoto, H.Y.: Biochemistry of the violaxanthin cycle in higher plants.-Pure appl. Chem. 51: 639-648, 1979. Go to original source...

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