biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:75-81, 2001 | DOI: 10.1023/A:1017922421606

Effect of Benzylaminopurine on Rehydration of Bean Plants after Water Stress

J. Rulcová1, J. Pospíšilová2
1 Faculty of Science, Department of Plant Physiology, Charles University, Prague 2, Czech Republic
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

The possibility of improving the recovery of plant photosynthesis after water stress by cytokinin-induced stimulation of stomatal opening or delay of leaf senescence was tested. The 6-benzylaminopurine (BAP) in concentrations 1 and 10 μM was applied to the substrate (sand + nutrient solution) or sprayed on primary leaves of 14-d-old Phaseolus vulgaris L. plants sufficiently supplied with water or water-stressed for 4 d. The later ones having relative water content decreased to 69 % were fully rehydrated during the following three days. Parameters of photosynthesis and water relations were measured in primary leaves of 7-, 10-, 14-, and 17-d-old plants. Application of 1 μM BAP slightly delayed leaf senescence: in 17-d-old control plants, net photosynthetic rate (PN) and chlorophyll (Chl) content, and when sprayed on leaves also some of Chl a fluorescence kinetic parameters of BAP-treated leaves were slightly higher than those of untreated leaves. Both types of application of 1 μM BAP slightly improved recovery of plants during rehydration after water stress in terms of increased gad, gab and PN, i.e., parameters which were markedly decreased by mild water stress. However, contents of Chl a, Chl b and carotenoids and parameters of Chl a fluorescence kinetic were not markedly affected by mild water stress and after rehydration were not stimulated by 1 μM BAP. 10 μM BAP had mostly negative effects on the parameters measured.

Keywords: chlorophyll content; chlorophyll fluorescence; net photosynthetic rate; Phaseolus vulgaris; stomatal conductance
Subjects: bean; chlorophylls, water stress, rehydration; cytokinins; fluorescence, chlorophyll; gas exchange, water stress, rehydration; Phaseolus vulgaris; rehydration after water stress; stomatal conductance, water stress

Published: March 1, 2001  Show citation

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Rulcová, J., & Pospíšilová, J. (2001). Effect of Benzylaminopurine on Rehydration of Bean Plants after Water Stress. Biologia plantarum, 44(1), 75-81. doi: 10.1023/A:1017922421606
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