biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:83-87, 2001 | DOI: 10.1023/A:1017974405676

Changes in Mulberry Leaf Metabolism in Response to Water Stress

P. Barathi1, D. Sundar1, A. Ramachandra Reddy1
1 School of Life Sciences, Pondicherry University, Pondicherry-, India

A series of experiments were conducted to characterize the water stress-induced changes in the activities of RuBP carboxylase (RuBPCO) and sucrose phosphate synthase (SPS), photosystem 2 activity, and contents of chlorophylls, carotenoids, starch, sucrose, amino acids, free proline, proteins and nucleic acids in mulberry (Morus alba L. cv. K-2) leaves. Water stress progressively reduced the activities of RuBPCO and SPS in the leaf extracts, the chlorophyll content, and PS2 activity in isolated chloroplasts. Plants exposed to drought showed lower content of starch and sucrose but higher total sugar content than control plants. While the soluble protein content decreased under water stress, the amino acid content increased. Proline accumulation (2.5-fold) was noticed in stressed leaves. A reduction in the contents of DNA and RNA was observed. Reduced nitrogen content was associated with the reduction in nitrate reductase activity. SDS-PAGE protein profile showed few additional proteins (78 and 92 kDa) in the water stressed plants compared to control plants.

Keywords: chlorophyll; Morus alba; nucleic acids; photosynthesis; photosystem 2 activity; proteins; RuBP carboxylase; sucrose phosphate synthase
Subjects: chlorophylls, water stress, rehydration; Morus alba; mulberry; nucleic acids, water stress; photosystem 2, water stress; proteins, water stress; rehydration after water stress; ribulose-1,5-bisphosphate carboxylase/oxygenase, water stress; sucrose phosphate synthase, water stress; sugars, photosynthesis, water stress; water stress, photosynthesis

Published: March 1, 2001  Show citation

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Barathi, P., Sundar, D., & Ramachandra Reddy, A. (2001). Changes in Mulberry Leaf Metabolism in Response to Water Stress. Biologia plantarum, 44(1), 83-87. doi: 10.1023/A:1017974405676
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