biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 45:593-596, 2002 | DOI: 10.1023/A:1022390917656

Influence of Brassinosteroids on Antioxidant Enzymes Activity in Tomato Under Different Temperatures

L.M. Mazorra1, M. Núñez1, M. Hechavarria2, F. Coll2, M.J. Sánchez-Blanco3
1 Dpto. Fisiologia y Bioquímica Vegetal, Instituto Nacional de Ciencias Agrícolas (INCA), La Habana, Cuba
2 Laboratorio de Productos Naturales, Facultad de Química, Universidad de La Habana, La Habana, Cuba
3 Dpto. Riego y Salinidad, Centro de Edafología y Biología Aplicada del Segura (CSIC), Murcia, Spain

The effect of brassinosteroids (BRs) on catalase (EC 1.11.1.6), peroxidase (EC 1.11.1.7) and superoxide dismutase (SOD, EC 1.15.1.1) activity in tomato leaf discs was analyzed at 25 and 40 °C. Tomato leaf discs were preincubated for 24 h in Petri dishes with 24-epibrassinolide (EBR) or a polyhydroxylated spirostanic analogue of brassinosteroids (MH5). Both concentrations (10.60 and 2.12 nM) of EBR and MH5 stimulated the activity of SOD at 25 and 40 °C, the MH5-stimulated increase of this enzyme activity was greater. Peroxidase activity was unaffected at 25 °C, while at 40 °C this activity was enhanced by both compounds. The changes in catalase activity markedly depended on the structure BRs, doses and temperature. The results suggest a possible role of EBR and MH5 in the reduction of cell damage produced by heat stress due to induction of enzymatic antioxidants.

Keywords: brassinosteroid analogue; catalase; heat stress; Lycopersicon esculentum; peroxidase; superoxide dismutase
Subjects: antioxidants; brassinosteroids, antioxidant enzymes; catalase; heat stress, brassinosteroids, antioxidant enzymes; Lycopersicon esculentum; peroxidase; superoxide dismutase, brassinosteroids; temperature, brassinosteroids, antioxidant enzymes; tomato, brassinosteroids, antioxidant enzymes

Published: December 1, 2002  Show citation

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Mazorra, L.M., Núñez, M., Hechavarria, M., Coll, F., & Sánchez-Blanco, M.J. (2002). Influence of Brassinosteroids on Antioxidant Enzymes Activity in Tomato Under Different Temperatures. Biologia plantarum, 45(4), 593-596. doi: 10.1023/A:1022390917656
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