biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:149-152, 2003 | DOI: 10.1023/A:1027313805930

Induction of Betalain Pigmentation in Hairy Roots of Red Beet under Different Radiation Sources

K.S. Shin1, H.N. Murthy1,2, J.W. Heo1, K.Y. Paek1
1 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Korea
2 Karantak University, Dharwad, India

The effect of different radiation sources - blue (B), red (R), R plus B (RB), B plus far red (BFr), R plus far red (RFr) - was tested on the growth of hairy roots and betalain accumulation in Beta vulgaris L. (red beet). Light emitting diodes were used as radiation sources. The growth of hairy roots under different radiation treatments depended on radiation quality. Highest biomass accumulation was under the BFr treatment. BFr treatment efficiently induced betalain pigmentation in hairy roots. Total sugar and sucrose contents of hairy roots were also greater in this treatment. Thus, the betalain pigmentation in the cultured hairy roots can be influenced by radiation quality and BFr is most suitable for accumulation of betalains.

Keywords: Beta vulgaris; betacyanin; betaxanthin; biomass; bioreactor; light emitting diodes; radiation quality
Subjects: Beta vulgaris; betacyanin; betalain; betaxanthin; bioreactor; hairy root; radiation, visible, quality, sources; red beet, betalain pigmentation, hairy roots; root, hairy

Published: July 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Shin, K.S., Murthy, H.N., Heo, J.W., & Paek, K.Y. (2003). Induction of Betalain Pigmentation in Hairy Roots of Red Beet under Different Radiation Sources. Biologia plantarum46(1), 149-152. doi: 10.1023/A:1027313805930
Download citation

References

  1. Bohm, H., Bohm, L., Rink, E.: Establishment and characterization of a betaxanthin producing cell cultures from Portulaca grandiflora.-Plant Cell Tissue Organ. Cult. 26: 75-82, 1991. Go to original source...
  2. Bohm, H., Rink, E.: Betalains.-In: Vasil, I.K. (ed.): Cell Culture and Somatic Cell Genetics of Plants. Vol 5. Pp. 449-463. Academic Press, San Diego-London 1988. Go to original source...
  3. Bianco-Colomos, J., Huges, M.: Establishment and characterization of a betacyanin producing cell line of Amaranthus tricolor: Inductive effect of light and cytokinin.-J. Plant Physiol. 136: 734-739, 1990. Go to original source...
  4. Buitelaar, R.M., Tramper, J.: Strategies to improve the production of secondary metabolites with plant cell cultures: A literature review.-J. Biotechnol. 23: 11-141, 1992. Go to original source...
  5. Chaplin, M.F.: Monosaccharides.-In: Chaplin, M.F., Kennedy, J.F. (ed.): Carbohydrate Analysis: A Practical Approach. Pp. 1-36. IRL Press, Oxford 1986.
  6. Hamill, J.D., Parr, A.J., Robins, R.J., Rhodes, M.J.C.: Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes.-Plant Cell Rep. 5: 111-114, 1986. Go to original source...
  7. Kendrick, R.E., Kronenberg, G.H.M.,: Photomorphogenesis in Plants.-Kluwer Academic Publishers, Dordrecht 1993. Go to original source...
  8. Kino-Oka, M., Hongo, Y., Taya, M., Tone, S.: Culture of red beet hairy root in bioreactor and recovery of pigment released from cells by repeated treatment of oxygen starvation.-J. chem. Eng. J. 25: 490-495, 1992. Go to original source...
  9. Kishima, Y., Shimaya, A., Adachi, T.: Evidence that blue light induces betalain pigmentation in Portulaca callus.-Plant Cell Tissue Organ Cult. 43: 67-70, 1995. Go to original source...
  10. Knobloch, K.H., Bast, G., Berlin, J.: Medium and light induced formation of serpentine and anthocyanins in cell suspension cultures of Catharanthus roseus.-Phytochemistry 21: 591-594, 1982. Go to original source...
  11. Leathers, R.R., Davin, C., Zryd, P.: Betalain producing cell cultures of Beta vulgaris L. var. Bikores Monogerm (red beet).-In Vitro cell. dev. Biol. 28: 39-45, 1992. Go to original source...
  12. Matsumoto, T., Nishida, K., Noguchi, M., Tamaki, E.: Some factors affecting the anthocyanin formation by Populus cells in suspension culture.-Agr. biol. Chem. 37: 561-567, 1973. Go to original source...
  13. Mohr, H.: Coaction between pigment systems.-In: Kendrick, R.E., Kronenberg, G.H.M. (ed.): Photomorphogenesis in Plants. Pp. 547-564. Martinus Nijhoff, Dordrecht 1993. Go to original source...
  14. Mukundan, U., Bhide, V., Singh, G., Curtis, W.R.: pH mediated release of betalains from transformed root cultures of Beta vulgaris L.-Appl. Microbiol. Biotechnol. 50: 241-245, 1998b. Go to original source...
  15. Mukundan, U., Garvalho, E.B., Curtis, W.R.: Growth and pigment production by hairy root culture of Beta vulgaris L. in a bubble column reactor.-Biotechnol. Lett. 20: 469-474, 1998a. Go to original source...
  16. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
  17. Nilsson, T.: Studies into the pigments in beet root (Beta vulgaris L. sp. vulgaris var. rubra L.).-Lantbrukshogskolans Ann. 36: 179-219, 1970.
  18. Obrenovic, S.: Effect of riboflavin and cyamide on blue light induction of betacyanin formation in Amaranthus caudatus seedlings.-Physiol. Plant. 65: 418-422, 1985. Go to original source...
  19. Sato, K., Nakayama, M., Shigeta, J.: Culturing conditions affecting the production of anthocyanin in suspended cell cultures of strawberry.-Plant Sci. 113: 91-98, 1996. Go to original source...
  20. Taya, M., Mine, K., Kino-Oka, M., Tone, S., Ichi, T.: Production and release of pigments by culture of transformed hairy root of red beet.-J. Ferment. Bioeng. 73: 31-36, 1992. Go to original source...
  21. Yamakawa, T., Kato, S., Ishida, K., Komada, H., Minoda, Y.: Production of anthocyanins by Vitis cells in suspension culture.-Agr. biol. Chem. 47: 2185-2191, 1983. Go to original source...
  22. Zhong, J.J., Seki, T., Kinoshita, S., Yoshida, T.: Effect of light irradiation on anthocyanin production by suspended cultures of Perilla frutescens.-Biotechnol. Bioeng. 38: 653-658, 1991. Go to original source...