biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:361-366, 2003 | DOI: 10.1023/B:BIOP.0000023878.58899.88

Changes in Tomato Leaves Induced by NaCl Stress: Leaf Organization and Cell Ultrastructure

O. Sam1, C. Ramírez2, M.J. Coronado2, P.S. Testillano2, M.C. Risueño2,*
1 Dpto. de Fisiología y Bioquímica Vegetal, Instituto Nacional de Ciencias Agrícolas, La Habana, Cuba
2 Plant Development and Nuclear Organization, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain

The alterations of organization of leaf tissues and cell ultrastructure as a consequence of salt stress (75 and 150 mM NaCl) were studied in two tomato (Lycopersicum esculentum Mill.) cultivars showing different salinity tolerance. The salinity brought changes in cell shape, volume of intercellular spaces and chloroplast number, shape and size. These characteristics were specific in each cultivar. The ultrastructural changes were also different in the two tomato cultivars studied and the most important ones were in the number and size of starch granules in chloroplasts, the number of electron-dense corpuscules in the cytoplasm, the structure of mitochondria, and number of plastoglobuli.

Keywords: chloroplasts; Lycopersicum esculentum; mitochondria; plastoglobuli
Subjects: chloroplast, NaCl stress; cultivar and genotype differences, ultrastructure, leaf structure; Lycopersicon esculentum; mitochondrion, NaCl stress; plastoglobuli, NaCl stress; salt stress, ultrastructure, leaf structure; tomato, cell ultrastructure

Published: November 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Sam, O., Ramírez, C., Coronado, M.J., Testillano, P.S., & Risueño, M.C. (2003). Changes in Tomato Leaves Induced by NaCl Stress: Leaf Organization and Cell Ultrastructure. Biologia plantarum, 46(3), 361-366. doi: 10.1023/B:BIOP.0000023878.58899.88
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Allakhverdiev, S.I., Sakamoto, A., Nishiyama, Y., Inaba, M., Murata, N.: Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.-Plant Physiol. 123: 1047-1056, 2000. Go to original source...
    2. Babber, S., Sheokand, S., Malik, S.: Nodule structure and functioning in chickpea (Cicer arietinum) as affected by salt stress.-Biol. Plant. 43: 269-273, 2000. Go to original source...
    3. Bradford, M.M.: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem 72: 248-254, 1976. Go to original source...
    4. Burssens, S., Himanen, K., Van de Cotte, B., Beeckman, T., Van Montagu, M., Inze, D., Verbruggen, N.: Expression of cell cycle regulatory genes and morphological alterations in response to salt stress in Arabidopsis thaliana.-Planta 211: 632-640, 2000. Go to original source...
    5. Cramer, G.R., Alberico, G.J., Schmidt, C.: Leaf expression limits dry matter accumulation of salt-stressed maize.-Amer. J. Bot. 21: 663-674, 1994. Go to original source...
    6. Dekov, I., Tsonev, T., Yordanov, I.: Effects of water stress and high-temperature stress on the structure and activity of photosynthetic apparatus of Zea mays and Helianthus annuus.-Photosynthetica 38: 361-366, 2000. Go to original source...
    7. Delfine, S., Alvino, A., Zacchini, M., Loreto, F.: Consequence of salt stress on conductance to CO2 diffusion, Rubisco characteristics and anatomy of spinach leaves.-Aust. J. Plant Physiol. 25: 395-402, 1998. Go to original source...
    8. El-Aref, H.M., Hamada, A.M.: Genotypic differences and alterations of protein patterns of tomato explants under copper stress.-Biol. Plant. 44: 555-564, 1998. Go to original source...
    9. Gaxiola, R., De Larrinoa, I.F., Villalba, J.M., Serrano, R.: A novel and conserved salt-induced protein is an important determinant of salt tolerance in yeast.-EMBO J. 11: 3157-3164, 1992. Go to original source...
    10. Gil-Mascarell, R., Lòpez-Coronado, J.M., Bellès, J.M., Serrano, R., Rodríguez, P.L.: The Arabidopsis HAL2-like gene family includes a novel sodium-sensitive phosphatase.-Plant J. 17: 373-383, 1999. Go to original source...
    11. Gong, Z., Koiwa, H., Cushman, M.A., Ray, A., Bufford, D., Korzeda, S., Matsumoto, T.K., Zhu, J., Cushman, J.C., Bressan, R.A., Hasegawa, P.M.: Genes that are uniquely stress regulated in salt overly sensitive (SOS) mutants.-Plant Physiol. 126: 363-375, 2001. Go to original source...
    12. Guerrier, G.: Proline accumulation in leaves of NaCl-sensitive and NaCl-tolerant tomatoes.-Biol. Plant. 40: 623-628, 1998. Go to original source...
    13. Hassanein, A.M.: Alterations in protein and esterase patterns of peanut in response to salinity stress.-Biol. Plant. 42: 241-248, 1999. Go to original source...
    14. Hernández, J.A., Almanza, M.S.: Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves.-Physiol. Plant. 115: 251-257, 2002. Go to original source...
    15. Hernández, J.A., Ferrer, M.A., Jimenez, A. Ros-Barcelò, A., Sevilla, F.: Antioxidant systems and O2 -/H2O2 production in the apoplast of Pisum sativum L. leaves: its relation with NaCl-induced necrotic lesions in minor veins.-Plant Physiol. 127: 817-831, 2001. Go to original source...
    16. Lechno, S., Zamski, E., Tell-Or, E.: Salt stress-induced responses in cucumber plants.-J. Plant Physiol. 150: 206-211, 1997. Go to original source...
    17. Makela, P., Karkkainen, J., Somersalo, S.: Effect of glycinebetaine on chloroplast ultrastructure, chlorophyll and protein content, and RuBPCO activities in tomato grown under drought or salinity.-Biol. Plant. 43: 471-475, 2000. Go to original source...
    18. Mansour, M.M.F., Van Hasselt, P.R., Kuiper, P.J.C.: NaCl effects on root plasma membrane ATPase of salt tolerant wheat.-Biol. Plant. 43: 61-66, 2000. Go to original source...
    19. McKersie, B.D., Leshem, Y.Y.: Stress and Stress Coping in Cultivated Plants.-Kluwer Academic Publishers, Dordrecht 1994. Go to original source...
    20. Muthukumarasamy, M., Dutta Gupta, S., Panneerselvam, R.: Enhancement of peroxidase, polyphenol oxidase and superoxide dismutase activities by triadimefon in NaCl stressed Raphanus sativus L.-Biol. Plant. 43: 317-320, 2000. Go to original source...
    21. Niu, X., Damsz, B., Kononowicz, A.K., Bressan, R.A., Hasegawa, P.M.: NaCl-induced alterations in both cell structure and tissue-specific plasma membrane H+-ATPase gene expression.-Plant Physiol. 111: 679-686, 1996. Go to original source...
    22. Paakkönen, H., Vahala, J., Pohjolai, M., Holopainen, T., Karenlampe, L.: Physiological, stomatal and ultrastructural ozone responses in birch (Betula pendula Roth.) are modified by water stress.-Plant Cell Environ. 21: 671-684, 1998. Go to original source...
    23. Rusch, T., Kirsch, M., Löw, R., Lehr, A., Virreck, R., Zhigang, A.: Salt stress responses of higher plants: the role of proton pumps and Na+/H+ antiporters.-J. Plant Physiol. 148: 425-433, 1996. Go to original source...
    24. Sam, O., Jerèz, E., Dell'Amico, J., Ruiz-Sanchez, M.C.: Water stress induced changes in the anatomy of tomato leaf epidermis.-Biol. Plant. 43: 275-277, 2000. Go to original source...
    25. Sam, O., Nuñez, M., Ruiz-Sánchez, M.C., Dell'Amico, J., Falcòn, V., De La Rosa, M.C., Seoane, J.: Effect of a brassinosteroid analogue and high temperature stress on leaf ultrastructure of Lycopersicon esculentum.-Biol. Plant. 44: 213-218, 2001. Go to original source...
    26. Serraj, R., Fleurat-Lessard, P., Jaillard, B., Drevon, J.J.: Structural changes in the inner-cortex cells of soybean root nodules are induced by short-term exposure to high salt or oxygen concentrations.-Plant Cell Environ. 18: 455-462, 1995. Go to original source...
    27. Testillano, P.S., Sánchez-Pina, M.A., Olmedilla, A., Fuchs, J.P., Risueño, M.C.: Characterization of the interchromatin region as the nuclear domain containing snRNPs in plant cells. A cytochemical and immunoelectron microscopy study.-Eur. J. Cell Biol. 61: 349-361, 1993.
    28. Voronin, P.Y., Manzhulin, A.V., Myasoedov, N.A., Balnokin, Y.V., Terentieva, E.I.: Morphological types and photosynthesis of C4 plants leaves under long-term soil salinity.-Russ. J. Plant Physiol. 42: 310-320, 1995.

    Return to the content