Genetic divergence and population structure of black sea bream Acanthopagrus schlegeli inferred from microsatellite analysis

DAL-SANG JEONG; TETSUYA UMINO; KENJI KURODA; MAREHIKO HAYASHI; HEISUKE NAKAGAWA; JU-CHAN KANG; KAGAYAKI MORISHIMA; KATSUTOSHI ARAI

doi:10.1046/j.1444-2906.2003.00705.x

Genetic divergence and population structure of black sea bream Acanthopagrus schlegeli inferred from microsatellite analysis

DAL-SANG JEONG, TETSUYA UMINO, KENJI KURODA, MAREHIKO HAYASHI, HEISUKE NAKAGAWA, JU-CHAN KANG, KAGAYAKI MORISHIMA, KATSUTOSHI ARAI

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Keywords: Acanthopagrus schlegeli, black sea bream, genetic variability, Mendelian inheritance, microsatellite, population structure

JOURNAL FREE ACCESS

2003 Volume 69 Issue 5 Pages 896-902

DOI https://doi.org/10.1046/j.1444-2906.2003.00705.x

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Abstract

Four highly variable microsatellite loci, Acs1*, Acs3*, Acs4* and Acs9*, were isolated and then used to investigate genetic diversity and population structure in black sea bream, Acanthopagrus schlegeli, collected from wild populations of six locations (western Japan and southern Korea), and hatchery stock and post-stock populations from Hiroshima Bay. When allelic segregation of each microsatellite locus was examined using the single spawning pair and their progeny, all loci were well fitted to the Mendelian manner of inheritance. The genetic variations at these loci in the eight populations revealed high levels of variability. In the wild populations the mean number of alleles per locus was 10.8-13.5, and the mean observed hetrozygosity was 0.755-0.828. In contrast, these values in hatchery stock population were 10.0 and 0.776, respectively. Genetic distance indicates the existence of geographic divergence between western Japan and south Korea, and little genetic differentiation among populations in western Japan.

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