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Volume 43, Issue 1

Phylogenetic Relationships of Marine Bacteria, Mainly Members of the Family , Determined on the Basis of 16S rRNA Sequences Free

Abstract

The phylogenetic relationships of 50 reference strains, mostly marine bacteria which require Na for growth, were determined on the basis of 600 16S rRNA nucleotides by using reverse transcriptase sequencing. Strains belonging to 10 genera were included (four genera of the family , the genus of the family , and the genera , and ). The sequences were alligned, the similarity values and evolutionary distance values were determined, and a phylogenetic tree was constructed by using the neighbor-joining method. On the basis of our results, the family was separated into at least seven groups (genera and families). clearly was on a line of descent that was remote from other vibrios. As determined by the similarity and evolutionary distance values, is more distantly related to the family than the members of the are. Also, strains formed a separate group with at the genus level. Of 30 species of the , 17 formed a large phylogenetic cluster. The genus was found to be a heterogeneous group, and the species were distributed in various subgroups of the . The separation of the family from the family and the separation of the genera and from the genus were confirmed in this phylogenetic study. However, a marine species, , was clearly separated from two terrestrial species. Each group that was separated by the phylogenetic analysis had characteristic 16S rRNA sequence patterns that were common only to species in that group. Therefore, the characteristic sequences described in this paper may be useful for identification purposes.

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1993-01-01
2024-04-23
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Phylogenetic Relationships of Marine Bacteria, Mainly Members of the Family Vibrionaceae, Determined on the Basis of 16S rRNA Sequences
Int J Syst Evol Microbiol 43, 8 (1993); https://doi.org/10.1099/00207713-43-1-8
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