1887

Abstract

Eight strains of mollicutes were isolated from pooled suspensions prepared from western black-legged ticks () collected in Oregon. Morphologic examination by electron and dark-field microscopic techniques showed that each strain consisted of a mixture of motile, tightly coiled helical cells, small coccoid cells with diameters ranging from 300 to 500 nm, and pleomorphic, straight or branched filamentous forms. All cellular forms were surrounded by a single cytoplasmic membrane, and there was no evidence of a cell wall. The organisms were filterable and fastidious in their growth requirements. The optimum temperature for growth was 30°C, but multiplication occurred at temperatures ranging from 23 to 32°C. The strains catabolized glucose but did not hydrolyze arginine or urea. The genome size of strain Y32 (T = type strain) was 2,220 kbp, and the DNA base composition (guanine-plus-cytosine content) of this organism was 25 ± 1 mol%. The eight isolates were serologically related to each other but were not related to 37 other type or representative strains belonging to the genus . Strain Y32 (= ATCC 33835) is the type strain of sp. nov.

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1995-01-01
2024-03-28
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References

  1. Abalain-Colloc M. L., Williamson D. L., Carie P., Abalain J. H., Bonnet F., Tully J. G., Konai M., Whitcomb R. F., Bové J. M., Chastel C. 1993; Division of group XVI spiroplasmas into subgroups. Int. J. Syst. Bacteriol 43:342–346
    [Google Scholar]
  2. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bactériologie techniques for characterization of Mycoplasma species. Int. J. Syst. Bacteriol 20:35–58
    [Google Scholar]
  3. Arthur D. R., Snow K. R. 1968; Ixodespacificus Cooley and Kohls, 1943: its life-history and occurrence. Parasitology 58:893–906
    [Google Scholar]
  4. Brinton L. P., Burgdorfer W. 1976; Cellular and subcellular organization of the 277F agent, a spiroplasma from the rabbit tick Haemaphysalis leporispalustris (Aeari: Ixodidae). Int. J. Syst. Bacteriol 26:554–560
    [Google Scholar]
  5. Burgdorfer W., Lane R. S., Barbour A. G., Gresbrink R. A., Anderson J. R. 1985; The western black-legged tick, Ixodes pacificus: a vector of Borrelia burgdorferi . Am. J. Trop. Med. Hyg 34:925–930
    [Google Scholar]
  6. Carle P., Laigret F., Bové J. M., Tully J. G. Heterogeneity of genome sizes within the genus Spiroplasma . Submitted for publication
    [Google Scholar]
  7. Carle P., Rose D. L., Tully J. G., Bové J. M. 1992; The genome size of spiroplasmas and other mollicutes. IOM Lett 2:263
    [Google Scholar]
  8. Carle P., Saillard C., Bové J. M. 1983; DNA extraction and purification. xyoMethods Mycoplasmol 1:295–299
    [Google Scholar]
  9. Carle P., Saillard C., Bové J. M. 1983; Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol 1:301–308
    [Google Scholar]
  10. Chastel C, Humphrey-Smith I. 1991; Mosquito spiroplasmas. 149–206In Harris K. F. Advances in disease vector research 7: Springer-Verlag; New York:
    [Google Scholar]
  11. Clark T. B. 1983; Spiroplasmas: diversity of arthropod reservoirs and host-parasite relationships. Science 217:57–59
    [Google Scholar]
  12. Clark T. B., Whitcomb R. F., Tully J. G., Mouches C., Saillard C., Bové J. M., Wrôblewski H., Carle P., Rose D. L., Henegar R. B., Williamson D. L. 1985; Spiroplasma melliferum, a new species from the honeybee {Apis mellifera). Int. J. Syst. Bacteriol 35:296–308
    [Google Scholar]
  13. Clyde W. A. Jr 1983; Growth inhibition tests. Methods Mycoplasmol 1:405–410
    [Google Scholar]
  14. Easton E. R., Keirans J. E., Gresbrink R. A., Clifford C. M. 1977; The distribution in Oregon of Ixodes pacificus, Dermacentor andersoni Dermacentor occidentalis, with a note on Dermacentor variabilis (Acarina: Ixodidae). J. Med. Entomol 13:501–506
    [Google Scholar]
  15. French F. E., Whitcomb R.F., Tully J. G., Hackett K. J., Clark E. A., Henegar R. B., Wagner A. G., Rose D. L. 1990; Tabanid spiroplasmas of the southeast USA: new groups, and correlation with host life history strategy. Zentralbl. Bakteriol. Suppl 20:919–921
    [Google Scholar]
  16. Freundt E. A., Andrews B. E., Ernø H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethol-sulfonate and digito-nin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg 225:104–112
    [Google Scholar]
  17. Gardella R. S., Del Giudice R. A. 1983; Hemagglutination, hemadsorption, and hemolysis. Methods Mycoplasmol 1:379–384
    [Google Scholar]
  18. Gasparich G. E., Saillard C., Clark E. A., Konai M., French F. E., Tully J. G., Hackett K. J., Whitcomb R. F. 1993; Sérologie and genomic relatedness of group VIII and group XVII spiroplasmas and subdivision of spiroplasma group VIII into subgroups. Int. J. Syst. Bacteriol 43:338–341
    [Google Scholar]
  19. Hackett K. J., Clark T. B. 1989; Ecology of spiroplasmas. 113–200 Whitcomb R. F., Tully J. G. The mycoplasmas 5: Academic Press; New York:
    [Google Scholar]
  20. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes. Int. J. Syst. Bacteriol 29:172–180
    [Google Scholar]
  21. Junca P., Saillard C., Tully J. G. O., Garcia-Jurado J. R., Degorce-Dumas C., Mouches Vignault J. C., Vogel R., McCoy R., Whitcomb R. F., Williamson D. L., La trille J., Bové J. M. 1980; Characterisation de spiroplas-mas isolés d’insectes et de fleurs de France continentale, de Corse et du Maroc. Proposition pour classification des spiroplasmas. C. R. Acad. Sci. Ser. D 290:1209–1212
    [Google Scholar]
  22. Manweiler S. A., Lane R. S., Block W. M., Morrison M. L. 1990; Survey of birds and lizards for ixodid tick (Acari) and spirochetal infection in northern California. J. Med. Entomol 27:1011–1015
    [Google Scholar]
  23. Manweiler S. A., Lane R. S., Tempelis C. H. 1992; The western fence lizard Sceloporus occidentalis: evidence of field exposure to Borrelia burgdorferi in relation to infestation by Ixodes pacifiais (Acari: Ixodidae). Am. J. Trop. Med. Hyg 47:328–336
    [Google Scholar]
  24. Mouches C, Bové J. M., Tully J. G., Rose D. L., McCoy R. E., Carle-Junca P., Gamier M., Saillard C. 1983; Spiroplasma apis, a new species from the honeybee (Apis mellifera) . Ann. Microbiol 134:383–397
    [Google Scholar]
  25. Pyle L. E., Corcoran L. N., Cocks B. G., Bergemann A. D., Whitley J. C., Finch L. R. 1988; Pulsed-field electrophoresis indicates larger-than-ex-pected sizes for mycoplasma genomes. Nucleic Acids Res 16:6015–6025
    [Google Scholar]
  26. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol 102:306–310
    [Google Scholar]
  27. Rose D. L., Tully J. G., Bové J. M., Whitcomb R. F. 1993; A test for measuring growth responses of mollicutes to serum and polyoxyethylene sorbitan. Int. J. Syst. Bacteriol 43:527–532
    [Google Scholar]
  28. Rose D. L., Tully J. G., Whitcomb R. F., Williamson D. L., Bové J. M. 1983; Unique characteristics of the Y32 group of spiroplasmas (group VI) recovered from Ixodes pacificus ticks. Yale J. Biol. Med 56:844–845
    [Google Scholar]
  29. Saglio P., Lhospital M., Laflèche D., Dupont G., Bové J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp. n.: a mycoplasma-like organism associated with “stubborn” disease of citrus. Int. J. Syst. Bacteriol 23:191–204
    [Google Scholar]
  30. Saillard C, Vignault J. C., Bové J. M., Raie A., Tully J. G., Williamson D. L., Fos A., Gamier M., Gadeau A., Carle P., Whitcomb R. F. 1987; Spiroplasma phoeniceum sp. nov., a new plant-pathogenic species from Syria. Int. J. Syst. Bacteriol 37:106–115
    [Google Scholar]
  31. Senterfit L. B. 1983; Preparation of antigens and antisera. Methods Mycoplasmol 1:401–404
    [Google Scholar]
  32. Tully J. G. 1983; Reflections on recovery of some fastidious mollicutes with implications of the changing host patterns of these organisms. Yale J. Biol. Med 56:799–813
    [Google Scholar]
  33. Tully J. G. 1983; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol 1:173–177
    [Google Scholar]
  34. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirement. Methods Mycoplasmol 1:355–362
    [Google Scholar]
  35. Tully J. G., Bové J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes: proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasma-tales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae fam. nov.) from helical species (Spiroplasmataceaé), and emended descriptions of the order Mycoplasmata-les, family Mycoplasmataceae. Int. J. Syst. Bacteriol 43:378–385
    [Google Scholar]
  36. Tully J. G., Rose D. L., Clark E., Carle P., Bové J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol 37:357–364
    [Google Scholar]
  37. Tully J. G., Rose D. L., Yunker C. E., Cory J., Whitcomb R. F., Williamson D. L. 1981; Helical mycoplasmas (spiroplasmas) from Ixodes ticks. Science 212:1043–1045
    [Google Scholar]
  38. Tully J. G., Whitcomb R. F. 1991; The genus Spiroplasma. 1960–1980 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes 2, 2. Springer-Verlag; New York:
    [Google Scholar]
  39. Tully J. G., Whitcomb R. F., Rose D. L., Bové J. M. 1982; Spiroplasma mirum, a new species from the rabbit tick (Haemaphysalis leporispalustris). Int. J. Syst. Bacteriol 32:92–100
    [Google Scholar]
  40. Tully J. G., Whitcomb R. F., Rose D. L., Williamson D. L., Bové J. M. 1983; Characterization and taxonomic status of tick spiroplasmas: a review. Yale J. Biol. Med 56:599–603
    [Google Scholar]
  41. Tully J. G., Whitcomb R. F., Williamson D. L., Clark H. F. 1976; Suckling mouse cataract agent is a helical wall-free prokaryote (spiroplasma) pathogenic for vertebrates. Nature London: 259:117–120
    [Google Scholar]
  42. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol 171:6455–6467
    [Google Scholar]
  43. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol 1:147–158
    [Google Scholar]
  44. Whitcomb R. F., Bové J. M., Chen T. A, Tully J. G., Williamson D. L. 1987; Proposed criteria for an interim serogroup classification for members of the genus Spiroplasma (class Mollicutes). Int. J. Syst. Bacteriol 37:82–84
    [Google Scholar]
  45. Whitcomb R. F., Hackett K. J., Tully J. G., Clark E. A., French F. E., Henegar R. B., Rose D. L., Wagner A. 1990; Tabanid spiroplasmas as a model for mollicute biogeography. Zentralbl. Hyg. Suppl 20:931–933
    [Google Scholar]
  46. Whitcomb R. F., Tully J. G. 1984; Family III. Spiroplasmataceaé Skripal 1983, 408. genus I. Spiroplasma. 781–787 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 Williams and Wilidns; Baltimore:
    [Google Scholar]
  47. Whitcomb R. F., Tully J. G., McCawley P., Rose D. L. 1982; Application of the growth inhibition test to Spiroplasma taxonomy. Int. J. Syst. Bacteriol 32:387–394
    [Google Scholar]
  48. Whitcomb R. F., Tully J. G., Williamson D. L., Bové J. M., French F. E., Konai M., Gasparich G., Abalain-Colloc C.M., Saillard C., Chastel P., Carle D. L., Rose R., Henegar E. A., Clark C., Hackett K. J. 1992; Revised classification of spiroplasmas. IOM Lett 2:134
    [Google Scholar]
  49. Williamson D. L., Tully J. G. 1983; Guinea pig complement inhibits growth of Ixodes tick-derived Y32 spiroplasmas. Yale J. Biol. Med 56:845–846
    [Google Scholar]
  50. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and inhibition tests. Int. J. Syst. Bacteriol 29:345–351
    [Google Scholar]
  51. Williamson D. L., Tully J. G., Whitcomb R. F. 1989; The genus Spiroplasma. 71–111In Whitcomb R. F., Tully J. G. The mycoplasmas 5 Academic Press; New York:
    [Google Scholar]
  52. Williamson D. L., Whitcomb R. F., Tully J. G. 1978; The spiroplasma deformation test, a new serological method. Curr. Microbiol 1:203–207
    [Google Scholar]
  53. Yunker C. E., Tully J. G., Cory J. 1987; Arthropod cell lines in the isolation and propagation of tickborne spiroplasmas. Curr. Microbiol 15:45–50
    [Google Scholar]
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