1887

Abstract

A full-length DNA copy of cymbidium ringspot virus RNA was cloned downstream of a phage T7 promoter. transcripts had no extra nucleotides at the 3′ terminus, and a 5′ end likely to be precisely as in genomic RNA. Transcripts were infective when inoculated into test plants. Northern blots from inoculated plants revealed the presence of genomic and subgenomic RNAs, but not of satellite RNA. Virus particles isolated from infected plants had the same outward aspect and size as those of the wild-type virus and were decorated by an antiserum to CyRSV in immune electron microscopy tests.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-8-1857
1990-08-01
2024-04-30
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/8/JV0710081857.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-8-1857&mimeType=html&fmt=ahah

References

  1. Ahlquist P., Janda M. 1984; cDNA cloning and in vitrotranscription of the complete brome mosaic virus genome. Molecular and Cellular Biology 4:2876–2882
    [Google Scholar]
  2. Burgyan J., Russo M. 1988; Studies on the replication of a satellite RNA associated with cymbidium ringspot virus. Journal of General Virology 69:3089–3092
    [Google Scholar]
  3. Burgyan J., Russo M., Gallitelli D. 1986; Translation of cymbidium ringspot virus RNA in cowpea protoplasts and rabbit reticulocyte lysates. Journal of General Virology 67:1149–1160
    [Google Scholar]
  4. Dunn J. J., Studier F. W. 1983; Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. Journal of Molecular Biology 166:477–485
    [Google Scholar]
  5. Gallitelli D., Hull R. 1985; Characterization of satellite RNAs associated with tomato bushy stunt virus and five other definitive tombusviruses. Journal of General Virology 66:1533–1543
    [Google Scholar]
  6. Grieco F., Burgyan J., Russo M. 1989a; The nucleotide sequence of cymbidium ringspot virus RNA. Nucleic Acids Research 17:6383
    [Google Scholar]
  7. Grieco F., Burgyan J., Russo M. 1989b; Nucleotide sequence of the 3′-terminal region of cymbidium ringspot virus RNA. Journal of General Virology 70:2533–2538
    [Google Scholar]
  8. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  9. Heaton L. A., Carrington J. C., Morris T. J. 1989; Turnip crinkle virus infection from RNA synthesized in vitro . Virology 170:214–218
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Martelli G. P., Russo M., Gallitelli D. 1989; Tombusvirus group. AAB Descriptions of Plant Viruses352
    [Google Scholar]
  12. Milne R. G., Luisoni E. 1977; Rapid immune electron microscopy of virus preparations. Methods in Virology 6:265–281
    [Google Scholar]
  13. Rubino L., Burgyan J., Grieco F., Russo M. 1990; Sequence analysis of cymbidium ringspot virus satellite and defective interfering RNAs. Journal of General Virology 71:1655–1660
    [Google Scholar]
  14. Russo M., Burgyan J., Carrington J. C., Hillman B. I., Morris T. J. 1988; Complementary DNA cloning and characterization of cymbidium ringspot virus RNA. Journal of General Virology 69:401–406
    [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-8-1857
Loading
/content/journal/jgv/10.1099/0022-1317-71-8-1857
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error