1887

Abstract

The coat protein gene from potato virus S (PVS) was introduced into by leaf disc transformation using Transgenic plants expressing the viral coat protein were highly resistant to subsequent infection by the ME strain of PVS as indicated by an absence of symptom development and a lack of accumulation of virus in both the inoculated and upper leaves. As in reported experiments with plants expressing potato virus X coat protein, plants expressing PVS coat protein were also protected from inoculation with PVS RNA. These results provide further evidence that coat protein- mediated protection for these two groups of viruses, which share similar genome organizations, may involve inhibition of some early event in infection other than or in addition to virus uncoating.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-2167
1990-09-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710092167.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-2167&mimeType=html&fmt=ahah

References

  1. Bagnall R. H., Wetter C., Larson R. H. 1959; Differential host and serological relationships of potato virus M, potato virus S and carnation latent virus. Phytopathology 49:435–442
    [Google Scholar]
  2. Cuozzo M., O’Connell K. M., Kaniewski W., Fang R.-X., Chua N.-H., Tumer N. E. 1988; Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/Technology 6:549–557
    [Google Scholar]
  3. Dodds J. A., Lee S. Q., Tiffany M. 1985; Cross protection between strains of cucumber mosaic virus: effect of host and type of inoculum on accumulation of virions and double-stranded RNA of the challenge strain. Virology 144:301–309
    [Google Scholar]
  4. Fulton R. W. 1986; Practices and precautions in the use of cross protection for plant virus disease control. Annual Review of Phytopathology 24:67–81
    [Google Scholar]
  5. Hemenway C., Fang R.-X., Kaniewski W. K., Chua N.-H., Tumer N. E. 1988; Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO Journal 7:1273–1280
    [Google Scholar]
  6. Hoekema A., Huisman M. J., Molendijk L., van den Elzen P. J. M., Cornelissen B. J. C. 1989; The genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/Technology 7:273–278
    [Google Scholar]
  7. Horsch R. B., Fry J. E., Hoffmann N. L., Eichholtz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
  8. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  9. Kay R., Chan A., Daly M., Mcpherson J. 1987; Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236:1299–1302
    [Google Scholar]
  10. Koenig R. 1982; Carlavirus group. CMI/AAB Descriptions of Plant Viruses259
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  12. Lawson C., Kaniewski W., Haley L., Rozman R., Newell C., Sanders P., Tumer N. E. 1990; Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology 8:127–134
    [Google Scholar]
  13. Loesch-Fries L. S., Merlo D., Zinnen T., Burhop L., Hill K., Krahn K., Jarvis N., Nelson S., Halk E. 1987; Expression of alfalfa mosaic virus RNA4 in transgenic plants confers virus resistance. EMBO Journal 6:1845–1851
    [Google Scholar]
  14. Lok S., Abouhaidar M. G. 1986; The nucleotide sequence of the 5′ end of papaya mosaic virus RNA: site of in vitro assembly initiation. Virology 153:289–296
    [Google Scholar]
  15. Mackenzie D. J., Tremaine J. H., Stace-Smith R. 1989; Organization and interviral homologies of the 3′-terminal portion of potato virus S RNA. Journal of General Virology 70:1053–1063
    [Google Scholar]
  16. Mckinney H. H. 1929; Mosaic diseases in the Canary Islands, West Africa and Gibraltar. Journal of Agricultural Research 39:557–578
    [Google Scholar]
  17. Mackinnon J. P., Bagnall R. H. 1972; Use of N. debneyii to detect viruses X and Y in potato seed stocks and relative susceptibility of six common varieties to potato virus S. Potato Research 15:81–85
    [Google Scholar]
  18. Niblett C. L., Dickson E., Fernow K. H., Horst R. K., Zaitlin M. 1978; Cross protection among four viroids. Virology 91:198–203
    [Google Scholar]
  19. Powell-Abel P., Nelson R. S., De B., Hoffman N., Rogers S. G., Fraley R. T., Beachy R. N. 1986; Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743
    [Google Scholar]
  20. Rogers S. G., Horsch R. B., Fraley R. T. 1986; Gene transfer in plants: production of transformed plants using Ti plasmid vectors. Methods in Enzymology 118:627–640
    [Google Scholar]
  21. Rose D. G. 1983; Some properties of an unusual isolate of potato virus S. Potato Research 26:49–62
    [Google Scholar]
  22. Rupasov V. V., Morozov S. Yu., Kanyuka K. V., Zavriev S. K. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
    [Google Scholar]
  23. Sarkar S., Smitamana P. 1981; A proteinless mutant of tobacco mosaic virus: evidence against the role of a viral coat protein for interference. Molecular and General Genetics 184:158–159
    [Google Scholar]
  24. Sherwood J. L., Fulton R. W. 1982; The specific involvement of coat protein in tobacco mosaic virus cross protection. Virology 119:150–158
    [Google Scholar]
  25. Tumer N. E., O’Connell K. M., Nelson R. N., Sanders P. R., Beachy R. N., Fraley R. T., Shah D. M. 1987; Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO Journal 6:1181–1188
    [Google Scholar]
  26. Van Dun C. M. P., Bol J. F. 1988; Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167:649–652
    [Google Scholar]
  27. Van Dun C. M. P., Bol J. F., Van Vloten-Doting L. 1987; Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159:299–305
    [Google Scholar]
  28. Van Dun C. M. P., Overduin B., Van Vloten-Doting L., Bol J. 1988; Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. Virology 164:383–389
    [Google Scholar]
  29. Wilson T. M. A. 1984; Cotranslational disassembly of tobacco mosaic virus in vitro. Virology 137:255–265
    [Google Scholar]
  30. Wilson T. M. A. 1986; Expression of the large 5′-proximal cistron of tobacco mosaic virus by 70S ribosomes during cotranslational disassembly in a prokaryotic cell-free system. Virology 152:277–279
    [Google Scholar]
  31. Zimmern D. 1977; The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA. Cell 11:463–482
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-2167
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-2167
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error