1887

Abstract

We examined cytolytic cells that lyse pseudorabies virus (PRV)-infected cells in pigs. stimulation of peripheral blood mononuclear cells from PRV-immune pigs with live PRV generated cells that lysed PRV-infected immortalized B cells. Several lines of evidence indicated a major contribution of non-major histocompatibility complex (MHC)-restricted cytolytic cells, which displayed characteristics of natural killer (NK) or lymphokine-activated killer cells: cytolysis was non-MHC-restricted, depended on CD2CD4CD8 (or CD2CD4CD8) cells, was strongly augmented by antigenic stimulation and was not limited to virus-infected cells, i.e. the NK cell-susceptible target cell line K562 was also lysed. Cytolytic cells were also generated by antigenic stimulation with UV-inactivated PRV. Target cells transfected with and stably expressing PRV gB or gC were lysed to the same degree as PRV-infected target cells.

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1996-05-01
2024-03-29
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References

  1. Ben-Porat T., Demarchi J. M., Lomniczi B., Kaplan A. S. 1986; Role of glycoproteins of pseudorabies virus in eliciting neutralizing antibodies. Virology 154:325–334
    [Google Scholar]
  2. Bishop G. A., Kumel G., Schwartz S. A., Glorioso J. 1986; Specificity of human killer cells in limiting dilution culture for determinants of herpes simplex virus type 1 glycoproteins. Journal of Virology 57:264–300
    [Google Scholar]
  3. Eloit M., Fargeand D., L’Haridon R., Toma B. 1988; Identification of the pseudorabies glycoprotein gp50 as a major target of neutralizing antibodies. Archives of Virology 99:45–46
    [Google Scholar]
  4. Feduchi E., Alonso M. A., Carrasco L. 1989; Human gamma interferon and tumor necrosis factor exert a synergistic blockade on the replication of herpes simplex virus. Journal of Virology 63:1354–1359
    [Google Scholar]
  5. Fitzgerald-Bocarsly P., Howell D. M., Pettera L., Tehrani S., Lopez C. 1991; Immediate-early gene expression is sufficient for induction of natural killer cell-mediated lysis of herpes simplex virus type 1-infected fibroblasts. Journal of Virology 65:3151–3160
    [Google Scholar]
  6. Kaeffer B., Bottreau E., Phan Thanh L., Olivier M., Salmon H. 1990; Histocompatible miniature boar model: selection of transformed cell lines of B and T lineages producing retrovirus. International Journal of Cancer 46:481–488
    [Google Scholar]
  7. Kimman T. G., Bianchi A. T. J., Wensvoort G., De Bruin T. G. M., Meliefste C. 1993; Cellular immune response to hog cholera virus (HCV): T cells of immune pigs proliferate in vitro upon stimulation with live HCV, but the El envelope glycoprotein is not a major T-cell antigen. Journal of Virology 67:2922–2927
    [Google Scholar]
  8. Kimman T. G., De Bruin T. M. G., Voermans J. J. M., Peeters B. P. H., Bianchi A. T. J. 1995a; Development and antigen specificity of the lymphoproliferation response of pigs to pseudorabies virus: dichotomy between secondary B- and T-cell responses. Immunology 86:372–378
    [Google Scholar]
  9. Kimman T. G., Bianchi A. T. J., De Bruin T. G. M., Mulder W.A.M., Priem J., Voermans J. J. M. 1995b; Interaction of pseudorabies virus with immortalized porcine B cells: influence on surface class I and II major histocompatibility complex and immunoglobulin M expression. Veterinary Immunology and Immunopathology 45:253–263
    [Google Scholar]
  10. Lučin P., Jonjic S., Messerle M., Polic B., Hengel H., Koszinowski U. H. 1994; Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferongamma and tumour necrosis factor. Journal of General Virology 75:101–110
    [Google Scholar]
  11. Lunney J. K. 1993; Characterization of swine leukocyte differentiation antigens. Immunology Today 14:147–149
    [Google Scholar]
  12. Marchioli C. C., Yancey R. J., Timmins J. G., Post L. E., Young B. R., Povendo L. A. 1988; Protection of mice and swine from pseudorabies virus-induced mortality by administration of pseudorabies virus-specific mouse monoclonal antibodies. American Journal of Veterinary Research 49:860–864
    [Google Scholar]
  13. Mellencamp M. W., O’Brien P. C. M., Stevenson J. R. 1991; Pseudorabies virus-induced suppression of major histocompatibility complex class I antigen expression. Journal of Virology 65:3365–3368
    [Google Scholar]
  14. Moormann R. J. M., de Rover T., Briaire J., Peeters B. P. H., Gielkens A. L. J., van Oirschot J. T. 1990; Inactivation of the thymidine kinase gene of a gl deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain. Journal of General Virology 71:1591–1595
    [Google Scholar]
  15. Pescovitz M. D., Lunney J. K., Sachs D. H. 1985; Murine anti-T4 and T8 monoclonal antibodies: distribution and effects on proliferation and cytotoxic T cells. Journal of Immunology 134:37–44
    [Google Scholar]
  16. Pescovitz M. D., Lowman M. A., Sachs D. H. 1988; Expression of T-cell associated antigens by porcine natural killer cells. Immunology 65:267–271
    [Google Scholar]
  17. Saalmueller A., Hirt W., Maurer S., Weiland E. 1994; Discrimination between two subsets of porcine CD8+ cytolytic T lymphocytes by the expression of CD5 antigen. Immunology 81:578–583
    [Google Scholar]
  18. Sachs D. H., Leight G., Cone J., Schwarz S., Stuart L., Rosenberg S. 1976; Transplantation in miniature swine. I. Fixation of the major histocompatibility complex. Transplantation 22:559–567
    [Google Scholar]
  19. Van Oirschot J. T., Houwers D. J., Rziha H.-J., van Zaane D. 1988; Development of an ELISA for detection of antibodies to glycoprotein I of Aujeszky’s disease virus: a method for the serological differentiation between infected and vaccinated pigs. Journal of Virological Methods 22:191–206
    [Google Scholar]
  20. Zuckermann F. A., Zsak L., Mettenleiter T. C., Ben-Porat T. 1990; Pseudorabies virus glycoprotein gIII is a major target antigen for murine and swine virus-specific cytotoxic T lymphocytes. Journal of Virology 64:802–812
    [Google Scholar]
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