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Volume 146, Issue 11

Definition of the site of class 1 integrons Free

Abstract

Integron-encoded integrases recognize two distinct types of recombination site: sites, found in integrons, and members of the 59-base element (59-be) family, found in the integron-associated gene cassettes. The class 1 integron integrase, IntI1, catalyses recombination between and a 59-be, two 59-be, or two sites, but events involving two sites are less efficient than the reactions in which a 59-be participates. The full site is required for high-efficiency recombination with a 59-be site. It is 65 bp in length and includes a simple site, consisting of a pair of inversely oriented IntI1-binding domains, together with two further directly oriented IntI1-binding sites designated strong and weak. However, a smaller region that contains only the simple site is sufficient to support a lower level of recombination with a complete partner and the features that determine the orientation of reside within this region. An unusual reaction between the site and a 59-be appears to be responsible for the loss of the central region of a 59-be to create a potential fusion of two adjacent gene cassettes.

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  • Published Online:
Keyword(s): 2°rs, secondary recombination site , 59-be, 59-base element , 5′-CS, 5′ conserved segment , Ap, ampicillin , attI , Cm, chloramphenicol , gene cassettes , integrase , integron , IntI1, integrase of class 1 integrons , Nx, nalidixic acid , site-specific recombination , Sm, streptomycin , Su, sulphamethoxazole , Tc, tetracycline and Tp, trimethoprim
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2000-11-01
2024-04-18
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Definition of the attI1 site of class 1 integrons
Microbiology 146, 2855 (2000); https://doi.org/10.1099/00221287-146-11-2855
/content/journal/micro/10.1099/00221287-146-11-2855
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